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| Different Analysis of Leptin and Its Receptor in Skin of Different Parts in Young Yak (Bos grunniens) |
| ZHAO Peng-Fei1, CUI Yan1,2,*, YU Si-Jiu1,2, LIAO Bo1, HE Jun-Feng1, ZHANG Qian1, XU Hong-Wei1 |
1 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China;
2 Technology and Research Center of Gansu Province for Embryonic Engineering of Bovine and Sheep & Goat, Lanzhou 730070, China; |
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Abstract Leptin (leptin, LP) and its receptor leptin receptor (LEPR) play a key role in food intake and energy consumption control. The role of LP and LEPR in the growth of skin and hair follicles has been gradually proved. In order to explore the distribution characteristics of LP and LEPR in different parts of skin of young yak (Bos grunniens), and their possible roles in different parts of skin, 10 healthy young yaks were selected to collect back, abdomen, axilla and scrotum skin. The exact distribution and expression of LP and LEPR in the skin of back, abdomen, axilla and scrotum of young yaks were studied by qPCR, Western blot and immunohistochemical methods. The results of immunohistochemistry showed that the expression of LP and LEPR was basically the same, mainly distributed in the epidermis, outer root sheath of hair follicles, sweat glands, sebaceous glands, capillary endothelial cells and dermal fibroblasts. The average optical density results showed that the expression levels of LP and LEPR in abdomen and back were significantly higher than those in axilla and scrotum (P<0.05). The qPCR results showed that LP expression in the back and abdomen was significantly higher than that in the axilla and scrotum (P<0.05). The expression of LEPR in the back was significantly higher than that in the other 3 parts (P<0.05); There was no significant difference in abdominal, axilla and scrotum (P>0.05). Western blot results showed that LP expression in abdomen and back was significantly higher than that in axilla and scrotum, and the differences among different parts were significant (P<0.05). The expression of LEPR in the back and abdomen was significantly higher than that in the scrotum and axilla, and the difference was significant among different parts (P<0.05). In this study, the expression of LP and LEPR was detected in young yak skin. The expression of LP and LEPR was basically the same in different parts of skin, mainly in epidermis, outer root sheath of hair follicles, sebaceous glands, sweat glands, capillary endothelial cells and dermal inner layer fibroblasts, but the expression were significant differences in different parts of skin. The results suggest that LP and LEPR may play an important role in the growth of skin and hair follicles. This study provides some basic data for further exploring the regulation mechanism of yak skin and hair follicle growth in plateau environment.
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Received: 04 January 2022
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Corresponding Authors:
* cuiyan369@sina.com
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[1] 成令忠, 钟翠萍, 蔡文琴. 2003. 现代组织学[M]. 上海科学技术文献出版社, 上海, pp.707-733.
(Cheng L Z, Zhong C P,Cai W Q.2003. Modern histology[M]. Shanghai Scientific and Technological literature press, Shanghai, China, pp. 707-733.)
[2] 刘煜凡, 黄沙, 付小兵. 2020. 皮肤附属器汗腺发育及功能的机制研究[J]. 生命科学, 32(3): 219-226.
(Liu Y F, Huang S, Fu X B.2020. Skin appendage-sweat gland:Mechanism of development and function[J]. Chinese Bulletin of Life Sciences, 32(3): 219-226.)
[3] 王傲, 褚宏尚, 吴红光, 等. 2022. 瘦素受体在小鼠皮肤中表达及瘦素受体阳性细胞在皮肤发育和创伤愈合中的作用[J]. 中国组织工程研究, 26(25): 3993-3998.
(Wang A, Chu H S, Wu H G, et al.2022. Expression of leptin receptor in mouse skin and the role of leptin receptor-positive cells in skin development and wound healing[J]. Chinese Journal of Tissue Engineering Research, 26(25): 3993-3998.)
[4] 温旭红, 张高峰, 潘伦, 等. 2020. 瘦素对雄激素性脱发小鼠模型毛囊的影响[J]. 中国麻风皮肤病杂志, 36(8): 461-466.
(Wen X H, Zhang G F, Pan L, et al.2020. Effect of leptin on androgenetic alopecia mouse model[J]. China Journal of Leprosy and Skin Diseases, 36(8): 461-466.)
[5] 岳静. 2013. 不同年龄牦牛皮肤的组织结构观察[D]. 硕士学位论文, 甘肃农业大学, 导师: 崔燕. pp. 6-8.
(Yue J.2013. Observation the histological structure of skin in different age Yak (Bos grunniens)[D]. Thesis for M.S., Gansu Agricultural University, Supervisor: Cui Y, pp.6-8.)
[6] 张虔, 崔燕, 余四九, 等. 2021. 幼年牦牛不同部位皮肤毛囊的组织学结构及TGF-β2与HIF-1α差异性表达分析[J]. 畜牧兽医学报, 52(11): 3224-3233.
(Zhang Q, Cui Y, Yu S J, et al.2021. Histological of hair follicles from different parts of skin and expression of TGF-β2 and HIF-1α in young yak[J]. Acta Veterinaria et Zootechnica Sinica, 52(11): 3224-3233.)
[7] Brément T, Cossec C, Roux C, et al.2018. Expression of three adipokines (adiponectin, leptin and resistin) in normal canine skin: A pilot study[J]. Journal of Comparative Pathology, 167: 82-90.
[8] Bouloumie A, Drexler H, Lafontan M, et al.1998. Leptin, the product of ob gene, promotes angiogenesis[J]. Circulation Research, 83(10): 1059-1066.
[9] Camandola S.2018. Astrocytes, emerging stars of energy homeostasis[J]. Cell Stress, 2(10): 246-252.
[10] Chen H C, Smith S J, Tow B, et al.2002. Leptin modulates the effects of acyl CoA: Diacylglycerol acyltransferase deficiency on murine fur and sebaceous glands[J]. Journal of Clinical Investigation, 109(2): 175-181.
[11] Chen S C, Cunningham J J, Smeyne R J.2000. Expression of ob receptor splice variants during prenatal development of the mouse[J]. Journal of Receptor Research, 20(1): 87-103.
[12] Ezure T, Amano S.2010. Adiponectin and leptin up-regulate extracellular matrix production by dermal fibroblasts[J]. Biofactors, 31(3-4): 229-236.
[13] Farag A.2016. Immunohistochemical expression of leptin in non melanoma skin cancer[J]. Journal of Clinical & Diagnostic Research Jcdr, 10(8): 8-12.
[14] Frank S, Stallmeyer B, H Kämpfer, et al.2000. Leptin enhances wound re-epithelialization and constitutes a direct function of leptin in skin repair[J]. The Journal of Clinical Investigation, 106(4): 501-509.
[15] Gainsford T, Willson T A, Metcalf D, et al.1996. Leptin can induce proliferation, differentiation, and functional activation of hemopoietic cells[J]. Proceedings of the National Academy of Sciences of the USA, 93(25): 14564-14568.
[16] Guidi M C, Mirri C, Fratini E, et al.2012. In vivo skin leptin modulation after 14 MeV neutron irradiation: A molecular and FT-IR spectroscopic study[J]. Analytical and Bioanalytical Chemistry, 404(5):1317-1326.
[17] Hoggard N, Hunter L, Lea R G, et al.2000. Ontogeny of the expression of leptin and its receptor in the murine fetus and placenta[J]. British Journal of Nutrition, 83(03): 317-326.
[18] Iguchi M, Aiba S, Yoshino Y, et al.2001. Human follicular papilla cells carry out nonadipose tissue production of leptin[J]. Journal of Investigative Dermatology, 117(6): 1349-1356.
[19] Inagaki-Ohara K.2019. Gastric leptin and tumorigenesis: Beyond obesity[J]. International Journal of Molecular Sciences, 20(11): 2622.
[20] Islam M S, Morton N M, Hansson A, et al.1997. Rat insulinoma-derived pancreatic B-cells express a functional leptin receptor that mediates a proliferative response[J]. Biochemical and Biophysical Research Communications, 238(3): 851-855.
[21] Licursi V, Guidi M C, Vecchio G D, et al.2017. Leptin induction following irradiation is a conserved feature in mammalian epithelial cells and tissues[J]. International Journal of Radiation Biology, 93(9): 947-957.
[22] Mariona P, Catalina P, Andreu P.2018. Leptin as a breast milk component for the prevention of obesity[J]. Nutrition Reviews, 76(12): 875-892.
[23] Mercati F, Dall'Aglio C, Timperi L, et al.2019. Epithelial expression of the hormone leptin by bovine skin[J]. European Journal of Histochemistry Ejh, 63(1): 9-16.
[24] Murad A, Nath A K, Cha S T, et al.2003. Leptin is an autocrine/paracrine regulator of wound healing[J]. FASEB Journal, 17(13): 1895-1897.
[25] Poeggeler B, Schulz C, Pappolla M A, et al.2010. Leptin and the skin: A new frontier[J]. Experimental Dermatology, 19(1): 12-18.
[26] Santos-Alvarez J, Goberna R, V Sánchez-Margalet.1999. Human leptin stimulates proliferation and activation of human circulating monocytes[J]. Cellular Immunology, 194(1): 6-11.
[27] Schanton M, Maymó J L, Pérez-Pérez A, et al.2018. Involvement of leptin in the molecular physiology of the placenta[J]. Reproduction (Cambridge, England), 155(1): 1-12.
[28] Song L L, Cui Y, Yu S J, et al.2018. Expression characteristics of BMP2, BMPR-IA and Noggin in different stages of hair follicle in yak skin[J]. General and Comparative Endocrinology, 260: 18-24.
[29] Sumikawa Y, Inui S, Nakajima T, et al.2014. Hair cycle control by leptin as a new anagen inducer[J]. Experimental Dermatology, 23(1): 27-32.
[30] Tartglia L A, Dembski M, Weng X, et al.1995. Identification and expression cloning of a leptin receptor, OB-R[J]. Cell, 83(7): 1263-1271.
[31] Vuletic M, Jancic S, Milenkovic S, et al.2020. Clinical-pathological significance of Leptin receptor (LEPR) expression in squamous cell carcinoma of the skin[J]. Pathology-Research and Practice, 216(9): 153111.
[32] Xiang T, Yao M, Zhou Q, et al.2017. Serum and tissue leptin in lung cancer: A meta-analysis[J]. Oncotarget, 8(12): 19699-19711.
[33] Zhang Y, Proenca R, Maffei M, et al.1996. Positional cloning of the mouse obese gene an its human homologue[J]. Nature, 379(6505): 425-432.
[34] Zhou L, Jing J, Han W, et al.2018. Decorin promotes proliferation and migration of ORS keratinocytes and maintains hair anagen in mice[J]. Experimental Dermatology, 27(11): 1237-1244. |
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