Phenotypic Analysis and Gene Fine Mapping of A Semi-dwarf Multi-tiller Mutant in Rice (Oryza sativa)
CHEN Li-Ping1,2,*, JIANG Jia-Huan1,2,*, ZHU Yong-Sheng1,2, ZHENG Yan-Mei1,2, LIN Qiang1,2, XIE Hong-Guang1,2, WANG Ying-Heng1,2, CAI Qiu-Hua1,2, XIE Hua-An1,2,**, ZHANG Jian-Fu1,2,**
1 Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, China; 2 Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural, P.R. China/ Fuzhou Branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/ Fujian Key Laboratory of Rice Molecular Breeding/Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences & Technology/Base of South-China, National Key Laboratory of Hybrid Rice/National Rice Engineering Laboratory of China, Fuzhou 350003, China
Abstract Plant height and tiller number are important agronomic traits affecting rice (Oryza sativa) yield. Appropriate plant height and tiller number are beneficial to increase rice yield. In this study, a semi-dwarf mutant with multi-tiller, sd-fh7185 (semi-dwarf from Fuhui 7185), was screened from the mutant library of indica restorer line 'Fuhui 7185' (FH7185) with chemical mutating of 0.1% ethylmethylsulfone (EMS). The investigated and analyzed results showed that the plant height of sd-fh7185 decreased significantly at seedling stage, tillering stage and maturity stage compared with wild type FH7185 (P<0.01), the decrease of plant height was mainly caused by the shortening of panicle and the first internode, and sd-fh7185 was controlled by a single recessive nuclear gene located on chromosome 3 (Chr. 3) in rice. Map-based cloning results indicated that the target gene, sd-fh7185, was finally fine-mapped within a physical interval of about 120 kb between InDel markers ID73 and GH25 on Chr. 3. The target region contained 14 predicted functional genes, one of which was a documented dwarf multi-tiller gene tensinte branched 1 (OsTB1)/fine cuml 1 (FC1) (MSU-RGAP locus LOC_Os03g49880) in rice. The sequencing results revealed a single base substitution from C (FH7185, wild type, WT) to T (sd-fh7185) occurred at position 439 bp of the exon of LOC_Os03g49880 in sd-fh7185, which resulted in a premature termination, and the length of encoded product was shortened from 388 amino acids (WT) to 146 amino acids (sd-fh7185) with changed function. Summarily, sd-fh7185 was a newly identified allele of OsTB1/FC1 differed from previously reported fc1-1 and fc1-2, which enriched the semi-dwarf and multi-tillering gene resources in rice, and the results provide informative references for both the dissection of molecular mechanism and rice morphology breeding in rice.
CHEN Li-Ping,JIANG Jia-Huan,ZHU Yong-Sheng, et al. Phenotypic Analysis and Gene Fine Mapping of A Semi-dwarf Multi-tiller Mutant in Rice (Oryza sativa)[J]. 农业生物技术学报, 2023, 31(1): 15-24.
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