Abstract Long non-coding RNA (lncRNA) is known to be involved in adipogenesis, but the exact molecular mechanisms remain largely unknown. In order to further explore the potential role of lncRNAs in fat deposition and provide theoretical basis for beef cattle (Bos taurus) improvement, a total of 3 040 lncRNAs was identified in Nanyang and Pinan cattle back fat using high-throughput RNA sequencing technology, among which 3 008 (98.9%) lncRNAs were co-expressed by the two cattle. Characteristic analysis results showed that most (66.02%) lncRNAs were concentrated in length from 1 000 to 2 500 bp and distributed in all chromosomes. Differential expression analysis showed that there were 67 differentially expressed lncRNAs in Pinan cattle compared with Nanyang cattle, among which 32 were up-regulated and 35 were down-regulated. GO analysis results showed that differentially expressed lncRNAs were mainly enriched in lipids transport process, membrane composition and lipid antigen binding and other related biological processes. KEGG pathway analysis showed that these differentially expressed lncRNAs genes were mainly enriched in glycerophospholipid metabolism, ether lipid metabolism, triglyceride metabolism, carbohydrate digestion and absorption pathways. In order to explore the function mechanism of lncRNA, lncRNA-miRNA-mRNA control network was constructed. It was found that LOC104972839/LOC107131703-miR-27b-phospholipase A2 group IVF (PLA2G4F)/ major facilitator superfamily domain containing 2A (MFSD2A) might play an important role in fatty acid degradation and fat deposition. It can be used as a candidate target to study the mechanism of fat development. To test the reliability of sequencing results, qRT-PCR was used for expression level identification, and online software was used for coding ability prediction. The results showed that the expression level of differentially expressed lncRNAs was consistent with the RNA-sequencing results, and the coding ability was very low. In conclusion, this study provides new insights into the discovery and annotation of lncRNA related to fat deposition, and provides basis for further elucidation of the molecular regulatory mechanism of fat deposition in cattle.
ZHU Yun-Chang,SHAN Xin-Yue,WANG Hao-Jing, et al. Screening of Differentially Expressed LncRNA in Adipose Tissues of Pinan and Nanyang Cattle (Bos taurus) and Construction of Regulatory Networks[J]. 农业生物技术学报, 2022, 30(10): 1913-1925.
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