Association Analysis Between Polymorphisms in FABP4, FASN and TCAP Genes and Growth and Carcass Traits in Qinchuan Cattle (Bos taurus)
CHI Zhi-Jiao1,*, Chelmeg1,*, CHENG Gong2, SU Ya3, CHENG Li-Xin4, LIU Jian-Feng5, CAO Gui-Fang6, BAO Si-Qin1, ZAN Lin-Sen2,**, TONG Bin1,**
1 State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock/School of Life Sciences, Inner Mongolia University, Hohhot 010071, China; 2 National Beef Cattle Improvement Center/College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China; 3 Inner Mongolia Agricultural and Animal Husbandry-Tech Extension Center, Hohhot 010011, China; 4 Animal Husbandry Institute, Inner Mongolia Academy of Agricultural & Animal Husbandry, Hohhot 010031, China; 5 Key Laboratory of Animal Genetics, Breeding and Reproduction/National Engineering Laboratory for Animal Breeding, Ministry of Agriculture/College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; 6 Department of Veterinary, Inner Mongolia Agricultural University, Hohhot 010018, China
Abstract The growth rate and carcass quality of beef cattle (Bos taurus) have important economic value in beef cattle industry. Fat acid binding proteins 4 gene (FABP4) and fatty acid synthase gene (FASN) play important role in the process of fatty acid synthesis and fat deposition in beef cattle. Titin-cap (TCAP) gene is known to be a kind of muscle silk protein and plays important role in the assembly of myofibrils. There are a g.3691G>A SNP in FABP4 gene, a g.17924G>A SNP in FASN gene and a g.346G>A SNP in TCAP gene, which were associated with growth and carcass traits in beef cattle. The aims of this study were to investigate the genetic diversity of g.3691G>A, g.17924G>A and g.346G>A SNPs in Qinchuan cattle population, and confirm their association with growth and carcass traits in Qinchuan cattle. These 3 SNPs of 3 genes in 350 samples of Qinchuan cattle were genotyped by MassARRAY technology, and then the analyses of genetic diversity and association with growth and carcass traits in Qinchuan cattle were performed. The results showed that the genotype frequency of g.3691G>A SNP in FABP4 gene, g.17924G>A SNP in FASN gene and g.346G>A SNP in TCAP gene were consistent with Hardy-Weinberg equilibrium (P<0.05) in Qinchuan cattle population. Association analysis results showed that g.17924G>A SNP of FASN gene in Qinchuan cattle population was significantly associated with rump length and hip width (P<0.05), as well as beneficial alleles (G) frequency was significantly different from that of Korean and Angus cattle breeds (P<0.01). Association analysis results showed that g.3691G>A SNP of FABP4 gene in Qinchuan cattle population was significantly associated with hip height, hip width and chest circumference (P<0.05), as well as body weight, body length, rump length and chest depth (P<0.01), beneficial alleles (G) frequency was significantly different from that of Korean cattle (P<0.01). Association analysis results showed that g.346G>A SNP of TCAP gene in Qinchuan cattle population was significantly associated with withers height, hip height (P<0.01), as well as body weight, body length, pin bone width and intramuscular fat content (P<0.05), beneficial alleles (G) frequency was significantly different from that of Korean cattle (P<0.01). Thus, these results suggested that the g.3691G>A SNP in FABP4 gene, the g.17924G>A SNP in FASN gene and the g.346G>A SNP in TCAP gene could be an effective molecular marker for marker-assisted breeding of Qinchuan cattle, and these results could provide scientific basis for genetic improvement of Qinchuan cattle.
CHI Zhi-Jiao,Chelmeg,CHENG Gong, et al. Association Analysis Between Polymorphisms in FABP4, FASN and TCAP Genes and Growth and Carcass Traits in Qinchuan Cattle (Bos taurus)[J]. 农业生物技术学报, 2022, 30(8): 1488-1498.
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