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| Screening and Identification of Endophytic Bacteria BR-1 for the Biocontrol of Tomato (Solanum lycopersicum) Gray Mold and Analysis of Its Biocontrol Performance |
| LI La1, SHI Hong-Li1, YU Ting-Ting1, LIU Qing1, JIAN Wei1,*, YANG Xing-Yong1,2,* |
1 College of Life Sciences, Chongqing Normal University, Chongqing 401331, China;
2 School of Pharmacy, Chengdu University, Chengdu 601106, China |
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Abstract Screening endophytic bacteria with excellent antagonistic effect against Botrytis cinerea plays important roles in controlling of B. cinerea, increasing tomato (Solanum lycopersicum) yield and reducing postharvest tomato loss. The present study adopted LB medium and gradient dilution method to isolate endophytic bacteria from the roots of Stemona japonica, and the antagonistic strains were screened by plate confrontation method. The antagonistic strains were identified by morphological characteristics, physiological and biochemical analysis, and 16S rDNA sequence analysis. The bacteriostatic ability of the volatile compounds of antagonistic strains was measured by two-sealed-base-plates method. The control effect of antagonistic strains on B. cinerea and their colonization ability on tomato fruits were analyzed by using fruit-pricking method. Seed germination rate, root length, and bud length were measured by seed germination experiment to evaluate the growth promotion effect of antagonistic strains on tomato seedlings. Results indicated there was an excellent strain BR-1 was screened and identified as Burkholderia gladioli, which had nitrogen fixation and phosphorus solubilization effects. The results showed that BR-1 can inhibit the expansion of B.cinerea by inhibiting mycelial growth, and the inhibition rate of BR-1 could reach 73.38%, and further study showed that the volatiles of BR-1 strain exhibited significant antibacterial activity. The broad-spectrum antifungal experiments showed that BR-1 had different inhibitory effects on Fusarium moniliforme, Coccinea arachniformis, Phytophthora capsici, Fusarium oxysporum, Corynosporum cucurbitum and Alternaria brassicae, especially the inhibitory rate of F. moniliforme reached 72.97%. Seed germination experiment showed that BR-1 strain significantly improved the germination rate of tomato seeds, promoted the growth of tomato seedlings and successfully colonized tomato fruits. This study had isolated and screened a biocontrol endophytic bacterium BR-1 with significant disease resistance and growth-promoting effects, and provides important biological resources for the biocontrol of tomato gray mold.
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Received: 17 September 2021
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Corresponding Authors:
*jianwei7956@163.com; yangxy94@swu.edu.cn
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