Cloning, Expression and Interaction with AP2/ERF Transcription Factor Analysis of CAMTA1 Gene from Corchorus capsularis
ZHANG Gao-Yang1,*, DENG Jie-Lou1, XU Jian-Tang2,*, ZHANG Chao1, ZHOU Yue3, YE Shui-Feng1, WU Ying-Bao1, YANG Xin1, JIANG Lei1, HUANG Si-Qi4, LI De-Fang4
1 College of Life Sciences, Shangrao Normal University, Shangrao 334001, China; 2 College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3 Jiangxi Medical College, Shangrao 334001, China; 4 Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410000, China
Abstract Calmodulin-binding transcription factor (CAMTA) plays an important role in the regulation of plant growth and development and response to abiotic and biological stress. Jute (Corchorus capsularis) is an important commercial crop of bast fiber. In order to study the role of CAMTA in response to drought stress in jute, the 'Corchorus capsularis 179' was used as the material, primers were designed based on the sequence of CAMTA gene (CC.02G0003260) from the jute genome. The CAMTA gene was obtained by RT-PCR and named as CAMTA1 (OK415793). Sequence analysis showed that the CAMTA1 gene contained 13 exons and 12 introns, Its open reading frame was 3 051bp and encoded a protein containing 1 016 amino acids, which had the CG-1 domain, TIG domain, ANK repeat sequence and IQ motif of CAMTA proteins. Protein sequence alignment indicated that CAMTA1 had high homology with the protein of Malvaceae plants. qPCR analysis showed that CAMTA1 gene was expressed in root, stem, leaf and pericarp. The expression of CAMTA1 gene in leaves reached the highest at 6.0 h after drought stress induction. Fluorescence enzyme protein complementation assay suggested that CaMTA1 interacted with another stress-responsive transcription factor AP2/ERF to regulate plant growth, development and respond to both abiotic and biological stresses.These results provide a theoretical basis for further elucidating the molecular mechanism of CAMTA1 gene involved in plant drought response.
ZHANG Gao-Yang,DENG Jie-Lou,XU Jian-Tang, et al. Cloning, Expression and Interaction with AP2/ERF Transcription Factor Analysis of CAMTA1 Gene from Corchorus capsularis[J]. 农业生物技术学报, 2022, 30(6): 1087-1095.
[1] 安霞, 金关荣, 陈常理,等. 2019. 红麻、亚麻和黄麻种子萌发期的耐旱性比较[J]. 浙江农业科学, 60(1):49-50. (An X, Jin G R, Chen C L, et al.2019. Comparison of drought tolerance in seed germination of kenaf flax and jute[J]. Journal of Zhejiang Agricultural Sciences, 60(1):49-50.) [2] 黎栋, 杨健, 窦俊焕, 等. 2013. 黄麻幼苗期对干旱胁迫的形态生理响应及抗旱性评价[J]. 西南农业学报, 26(01): 125-130. (Li D, Yang J, Dou J H, et al.2013. Physiological response and drought-resistance evaluation of jute seedling under drought stress[J]. Southwest China Journal of Agricultural Sciences, 26(01): 125-130.) [3] 刘文宇, 杜何为, 黄敏. 2021. 玉米CAMTA基因家族的全基因组分析[J].分子植物育种, 19(11): 3499-3505. (Liu W Y, Du H W, Huang M.2021. Genome-wide analysis of the CAMTA gene family in maize (Zea mays L.)[J]. Molecular Plant Breeding, 19(11): 3499-3505.) [4] 王国平. 2015. 大豆CAMTA基因家族的鉴定及其对逆境的响应与拟南芥CAMTA3/SR1参与干旱反应的研究[D]. 硕士学位论文, 杭州师范大学, 导师: 杜立群, 曾后清, pp. 18. (Wang G P.2015. Identification of CaMTA gene family and its response to stress in soybean and the involvement of CaMTA3/SR1 in drought response in Arabidopsis thaliana[D].Thesis for M.S., Hangzhou Normal University, Supervisor: Du L Q, Zeng H Q, pp. 18.) [5] 徐益, 张力岚, 祁建民, 等. 2021. 主要麻类作物基因组学与遗传改良:现状与展望[J]. 作物学报, 47(06): 997-1019. (Xu Y, Zhang L L, Qian J M, et al.2021. Genomics and genetic improvement in main bast fiber crops: Advances and perspectives[J]. Acta Agronomica Sinica, 47(6):997-1019.) [6] 张静. 2019. 柑橘CAMTA基因家族的鉴定与功能的初步研究[D]. 硕士学位论文, 西南大学, 导师: 谢让金, pp. 2-3. (Zhang J.2019. Identification and function of CAMTA gene family in Citrus[D]. Thesis for M.S., Southwest University, Supervisor: Xie R J, pp. 2-3.) [7] Jacob F, Kracher B, Mine A, et al.2018. A dominant-interfering camta3 mutation compromises primary transcriptional outputs mediated by both cell surface and intracellular immune receptors in Arabidopsis thaliana[J]. The New phytologist, 217(4): 1667-1680. [8] Kabir S M T, Hossain M. S, Bashar K K, et al.2021. Genome-wide identification and expression profiling of AP2/ERF superfamily genes under stress conditions in dark jute (Corchorus olitorius L.)[J]. Industrial Crops & Products, 166: 113469. [9] Kim Y S, An C, Park S, et al.2017. CAMTA mediated regulation of salicylic a cid immunity pathway genes in Arabidopsis exposed to low temperatureand pathogen infection[J]. Plant Cell, 29(10):2465-2477. [10] Li X H, Huang L, Zhang Y F, et al.2014. Tomato SR/CAMTA transcription factors SlSR1 and SlSR3L negatively regulate disease resistance response and SlSR1L positively modulates drought stress tolerance[J]. BMC Plant Biology, 14(1): 286. [11] Marcec M J, Gilroy S, Poovaiah B W, et al.2019. Mutual interplay of Ca2+ and ROS signaling in plant immune response[J]. Plant Science, 283: 343-354. [12] Marcelo K L, Means A R, York B.2016. The Ca2+/calmodulin/CaMKK2 axis: Nature's metabolic CaMshaft[J]. Trends Endocrinol. Metabolism, 27(10): 706-718. [13] Pandey N, Ranjan A, Pant P, et al.2013. CAMTA1 regulates drought responses in Arabidopsis thaliana[J]. BMC Genomics, 14(14): 1471-2164. [14] Rahman H, Xu Y P, Zhang X R, et al.2016a. Brassica napus genome possesses extraordinary high number of CAMTA genes and CAMTA3 contributes to PAMP triggered immunity and resistance to Sclerotinia sclerotiorum[J]. Frontiers in Plant Science, 7: 581. [15] Rahman H, Yang J, Xu Y P.2016b. Phylogeny of plant CAMTAs and role of AtCAMTAs in nonhost resistance to Xanthomonas oryzae pv. Oryzae[J]. Frontiers in Plant Science, (7): 177. [16] Shen C J, Yang Y J, Du L Q, et al.2015. Calmodulin-binding transcription activators and perspectives for applications in biotechnology[J]. Applied Microbiology & Biotechnology, 99(24): 10379-10385. [17] Yael G, Roni A, Dikla N, et al.2010. Calmodulin-binding transcription activator 1 mediates auxin signaling and responds to stresses in Arabidopsis[J]. Planta Berlin, 232(1): 165-178. [18] Yuan P, Du L, Poovaiah B W.2018. Ca2+/calmodulin-dependent AtSR1/CAMTA3 plays critical roles in balancing plant growth and immunity[J]. International Journal of Molecular Sciences, 19(6): 104-113. [19] Yang F, Dong F S, Hu F H, et al.2020. Genome-wide identification and expression analysis of the calmodulin-binding transcription activator (CAMTA) gene family in wheat (Triticum aestivum L.)[J]. BMC Genetics, 21(1): 105. [20] Yang T, Poovaiah B W.2000. An early ethylene up-regulated gene encoding a calmodulin-binding protein involved in plant senescence and death[J]. Journal of Biological Chemistry, 275(49): 38467-38473. [21] Yongsig K, Sarah J, Gilmour L C, et al.2020. Arabidopsis CAMTA transcription factors regulate pipecolic acid biosynthesis and priming of immunity genes[J]. Molecular Plant, 13(1): 157-168. [22] Zhou L X, Yarra R.2021. Genome-wide identification and characterization of AP2/ERF transcription factor family genes in oil palm under abiotic stress conditions[J]. International Journal of Molecular Science, 22(6). 2821.
