藏猪肺组织新转录本注释及功能解析

doi:10.3969/j.issn.1674-7968.2022.03.007

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Abstract Tibetan pig (Sus scrofa) is a representative non model animal in plateau habitat. The study of its plateau response mechanism can provide a reference basis for the exploration of hypoxia adaptation mechanism of other animals. In order to improve and optimize the annotation information of Tibetan pig genome, analyze the characteristics of Tibetan pig lung tissue response to hypoxic environment at transcription level, this study performed RNA-seq sequencing of lung tissues in Tibetan pigs at different altitudes to construct cDNA library, and the reassembled sequencing data was compared and analyzed with the reference genome of Tibetan pigs. The results showed that the 5' end and 3' end of 334 genes were extended, and the 5' end of 1 313 genes and the 3' end of 2 734 genes were extended respectively; 809 new transcripts were excavated and 95 new transcripts at different altitudes were differentially expressed (P<0.05). GO analysis results showed that the differential new transcripts were significantly enriched in the process of cell metabolism, enzyme catalytic activity and cellular components (P<0.05); KEGG pathway enrichment results found that the differential new transcripts were significantly enriched (P<0.05) in the pathways such as programmed cell necrosis and nuclear factor-κB (NF-κB) signaling pathway. Nine new transcripts with significant differences were randomly selected for qPCR verification, and the results were consistent with that of RNA-seq. This study provides a useful supplement and reference for improving the annotation information of Tibetan pig genome and a reference basis for further studying the genetic mechanism of Tibetan pig adapting to different altitude environment.

Key words： Tibetan pig New transcript Gene structure optimization Hypoxic adaptability

Received: 29 June 2021

ZTFLH:

S828.2

Corresponding Authors: *zhaosg@gsau.edu.cn

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	Articles by authors
	YUAN Hao-Nan
	YANG Ya-Nan
	YANG Tian-Liang
	QUAN Jin-Qiang
	ZHAO Sheng-Guo

Cite this article:

YUAN Hao-Nan,YANG Ya-Nan,YANG Tian-Liang, et al. Annotations and Functional Analysis of New Transcripts from Tibetan Pig (Sus scrofa) Lung Tissue[J]. 农业生物技术学报, 2022, 30(3): 485-495.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2022.03.007 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2022/V30/I3/485

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