Identification of Mango (Mangifera indica) DBB Genes and Its Expression Analysis During the Infection of Colletotrichum gloeosporioides and Xanthomonas campestris pv. mangiferaeindicae
SUN Yu1,2, LIU Zhi-Xin2, YE Zi1,2, GAO Ai-Ping3, LUO Rui-Xiong3, PU Jin-Ji1,2, ZHANG He1,2,*
1 Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education/College of Plant Protection, Hainan University, Haikou 570228, China; 2 Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs/Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; 3 Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
Abstract B-box protein is a kind of zinc finger structure transcription factor, its function is universal and specific. In order to reveal the sequence and expression characteristics of the double B-box (DBB) gene family in Mangifera indica, in this study, based on the whole genome data of mango, using bioinformatics methods to analyze the sequence of mango DBB gene family, and the relative expression level of DBB gene family was studied by qRT-PCR during the infection of Colletotrichum gloeosporioides (Cg) and Xanthomonas campestris pv. mangiferaeindicae (Xcm). Nine DBB gene family members were identified from the mango genome and named MiDBB1~9. The 9 MiDBBs proteins were unstable hydrophilic acidic proteins, mainly located in the nucleus. Not only was the tertiary structures similar, but also the N-terminal were 2 conserved domains (B-box1 and B-box2) with the same site. According to protein structure prediction, phylogenetic relationship, conservative motifs and multiple sequence comparison analysis, it was inferred that the DBB gene family of mango was evolutionarily conserved in structure and similar in function. The phylogenetic tree constructed by mango with Arabidopsis thaliana, Nicotiana tabacum, Malus domestica, Ananas comosus and Populus trichocarpa showed that the DBB of mango were closely related to those of the P. trichocarpa, followed by apple. The results of qRT-PCR showed that during the infection of C. gloeosporioides, the relative expression of MiDBB1~9 continued to increase within 12~48 h. During the infection of X. campestris pv. mangiferaeindicae, the relative expression levels of MiDBB2 and MiDBB3 continued to increase within 12~72 h. It was preliminarily speculated that the expression of DBB gene in mangosteen was induced by C. gloeosporioides and X. campestris pv. mangiferaeindicae. The above findings provide reference for the follow-up study of the functions and mechanisms of mango DBB gene family members.
SUN Yu,LIU Zhi-Xin,YE Zi, et al. Identification of Mango (Mangifera indica) DBB Genes and Its Expression Analysis During the Infection of Colletotrichum gloeosporioides and Xanthomonas campestris pv. mangiferaeindicae[J]. 农业生物技术学报, 2021, 29(12): 2267-2278.
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