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| Analysis and Identification of miRNA in Skin Tissues of Tibetan Cashmere Goats (Capra hircus) with Different Cashmere Fineness |
| FU Xue-Feng1, ZHAO Bing-Ru2, SUO Lang-Da3, BA Gui3, DE Ji3, AWANG Cuo-Ji3, WU Yu-Jiang3,*, TIAN Ke-Chuan4,* |
1 Key Laboratory of Genetics Breeding and Reproduction of Xinjiang Wool-sheep & Cashmere-goat/Institute of Animal Science, Xinjiang Academy of Animal Sciences, Urumqi 830011, China;
2 College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
3 Institute of Animal Science, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850009, China;
4 Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China |
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Abstract As an important gene regulatory element, microRNA (miRNA) is expressed in various mammalian skin and hair follicles, regulating the growth and development of skin and hair at post-transcriptional level. The aim of present study was to analyze and identify miRNAs in the skin tissues of Tibetan cashmere goats, and explore the role and regulatory mechanisms of miRNAs in fiber diameter traits of Tibetan cashmere goats (Capra hircus). Four Tibetan cashmere goats with fineness cashmere (average fiber diameter (12.04±0.03) μm) and 4 goats with coarse cashmere (average fiber diameter (14.88±0.05) μm) were selected, respectively. Shoulder skin tissues were collected to extract RNA and established libraries for high-throughput sequencing. After the raw sequences were filtered and blasted with reference genome (ARS1), the miRNAs and other small RNAs were identified based on the known miRNAs in miRBase and the location information of other non-coding RNAs (ncRNAs) in the reference genome. The differentially expressed miRNAs in the skin tissues of Tibetan cashmere goats with different fiber diameters were screened out, and target-gene prediction and function analysis were performed. qPCR was used to detect the consistency of 5 miRNAs with the sequencing results. The results showed that each sample obtained at least 20.09 M raw reads and at least 19.46 M clean reads after filtering. A total of 426 known miRNAs and 119 novel miRNAs were identified. Twelve differentially expressed miRNAs were screened, of which 5 miRNAs were significantly up-regulated in the skin tissues of the goats with fineness cashmere when compared with that of coarse group, and 7 miRNAs were significantly down-regulated. Differentially expressed miRNAs were predicted to obtain a total of 621 target genes. KEGG enrichment analysis showed that the target genes were enriched in biological pathways such as B cell receptor signaling pathway, FcεRI signaling pathway, and T cell receptor signaling pathway. Meanwhile, qPCR results showed that the expression trends of selected 5 miRNAs were consistent with that of the sequencing results. The above results suggests the fineness of Tibetan cashmere might be regulated by different miRNAs, which further affecting the expression of target genes and simultaneously participating in the regulation of multiple signaling pathways. The present study provides a reference for deep researches on miRNAs and related signaling pathways regulating the fineness of cashmere.
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Received: 14 January 2021
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Corresponding Authors:
* wuyujiang_1979@163.com; tiankechuan@163.com
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