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| Tissue-specific Imprinting of Bovine (Bos taurus) ATP10A Gene |
| LIU Xiao-Qian1, DONG Yan-Qiu1, JIN Lan-Jie1, GU Shu-Kai1, LI Dong-Jie2, LI Shi-Jie1,* |
1 College of Life Science, Hebei Agricultural University, Baoding 071001, China;
2 College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China |
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Abstract Genomic imprinting is an important epigenetic modification mechanism in mammals, which is closely related to the growth and development of organisms. The ATP10A (ATPase phospholipid transporting 10A) located at one end of the PWS/AS imprinted region, which is related to the development of human (Homo sapiens) PWS (Prader-Willi syndrome) and AS (Angelman syndrome) syndromes. ATP10A is a brain-specific imprinted gene in human, but its imprinting status in cattle (Bos taurus) is still unknown. In this study, genomic DNA from 32 bovine heart tissues and 15 placentas were amplified by PCR first, and the single nucleotide polymorphic sites were found on ATP10A gene by direct sequencing of PCR products, the A/G heterozygous SNP locus (rs136134264) was identified on exon 20 of bovine ATP10A gene. Then total RNA in tissues and placenta of heterozygous cattle were used for RT-PCR to determine the allele expression status of ATP10A gene. The sequencing results of PCR products were compared with above heterozygous SNP locus, it was determined that ATP10A gene was monoallelic expression in bovine brain tissues and biallelic expression in other tissues and placenta. The DNA methylation status of the promoter region of ATP10A gene in bovine placenta and adult bovine tissues was further analyzed by bisulfite sequencing. It was found that the 46 CpG loci in the detected region showed hypomethylation in bovine tissues and placenta, indicating that the DNA methylation modification in the promoter region of ATP10A gene might not be involved in regulating imprinted gene expression of ATP10A. The present study provides a reference for further exploring of bovine ATP10A gene function and imprinting mechanism.
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Received: 25 March 2021
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Corresponding Authors:
* lishijie20005@163.com
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