中国荷斯坦牛<em>IGF1R</em>基因对产奶性状的遗传效应分析

doi:10.3969/j.issn.1674-7968.2021.08.008

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Abstract Previous study in this group discovered that the expression level of insulin-like growth factor Ⅰ (IGF1) receptor (IGF1R) gene in liver tissue of Chinese Holstein cows (Bos taurus) during the dry period was significantly higher than that in early and peak lactation periods through transcriptome sequencing. IGF1R is a receptor tyrosine kinase that plays a critical role in the signaling of the IGF family. With directly sequencing the encoding regions and 2 000 bp of upstream and downstream flanking regions of IGF1R gene using the pooled DNA from 40 sires to verify the IGF1R gene genetic effects on lactation traits in dairy cows. The identified SNPs were genotyped in 947 Chinese Holstein cows individually by target sequencing technology. Association analysis was conducted between the SNPs and 5 milk traits using the animal model. As a result, a total of 7 SNPs were detected including 1 SNP located in exon, 5 SNPs located in intron region and 1 SNP located in 3' flanking region. Of them, single-marker association analysis results showed that 6 SNPs were found associated with milk yield, fat yield, protein yield and protein percentage significantly (P=0.0146~<0.0001). The dominant effects or allelic substitution effects of 7 SNP sites reached significantly difference (P<0.05; P<0.01). Through linkage disequilibrium (LD) analysis, 7 SNPs were linked (r²=0.85~0.99) and formed 3 blocks. Haplotype-based association analysis showed that block 1 was not significantly associated with all the 5 milk traits (P>0.05). While block 2 and block 3 were associated with milk yield, fat yield and protein yield traits (P=0.0003~<0.0001), in which H1 and H2 were dominant haplotypes that increased milk yield, fat yield and protein yield, respectively. In conclusion, IGF1R gene had significant impacts on milk yield, fat yield and protein yield traits in dairy cow, implying it could be used as a molecular marker in genomic selection.

Key words： Chinese Holstein cow Insulin-like growth factor Ⅰ receptor (IGF1R) Milk production traits SNP Genetic effects

Received: 19 November 2020

ZTFLH:

S813.3

Corresponding Authors: * sundx@cau.edu.cn

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	Articles by authors
	LIU Ya-Nan
	PENG Peng
	ZHENG Wei-Jie
	HAN Bo
	YANG Chen-Dong
	LI Jian-Ming
	MA Ya-Bin
	JIANG Gui-E
	NI Jun-Qing
	SUN Dong-Xiao

Cite this article:

LIU Ya-Nan,PENG Peng,ZHENG Wei-Jie, et al. Genetic Effect Analysis of IGF1R Gene on Milk Production Traits in Chinese Holstein Cow (Bos taurus)[J]. 农业生物技术学报, 2021, 29(8): 1518-1527.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.08.008 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I8/1518

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