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| Effect of Heterologous Expression of StSWEET16b Gene on Fructose Content and Resistance to Late Blight in Tobacco (Nicotiana tabacum) |
| FAN Xi-De1, HE Miao-Miao1,2, YE Guang-Ji1,2, WANG Jian1,2, ZHOU Yun1,2,* |
1 Academy of Agriculture and Forestry Sciences,Qinghai University, Xining 810016, China;
2 Qinghai Academy of Agriculture and Forestry Sciences/State key Laboratory of Plateau Ecology and Agriculture, Qinghai University/Key Laboratory of Qinghai-Tibetan Plateau Biotechnology of Ministry of Education, Qinghai University/Key Laboratory of Potato Breeding of Qinghai, Xining 810016, China |
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Abstract Sugars will eventually be exported transporters (SWEETs), a novel family of membrane-transporters widely distributed in eukaryotes, which performed essential roles in plant growth and development processes, such as phloem loading for long distance sugar transport, hormone transport, pollen development, fruit development, seed filling and plant-pathogen interactions. To analyze the function of StSWEET16b in sugar transport and plant-pathogen interactions, in this study, potato (Solanum tuberosum 'Qingshu 9') was used as material, after treated with sucrose, fructose and glucose individually, the expression of StSWEET16b was analyzed by qRT-PCR in the leaves of 'Qingshu 9'. The expression pattern of StSWEET16b was changed under 3% sucrose, fructose and 3glucose treatments respective, suggesting that may be involved in the translocation of sucrose, fructose and glucose in potato. By analyzing the growth condition of the transgenic tobacco (Nicotiana benthamiana) on different sugar medium and measuring the contents of sucrose, fructose and glucose in the plants, it was found that the transgenic tobacco grew weaker than the wild-type plants on glucose and sucrose medium, and the contents of sucrose and glucose in the transgenic plants were lower than those in the wild-type plants. However, transgenic tobacco grew significantly better than wild-type plants on fructose medium (P<0.05), and the fructose content of transgenic plants was higher than that of wild-type plants, indicated that the heterologous expression of StSWEET16b in tobacco could promote fructose transport, and inhibit the transport of sucrose and glucose. Transient expression results showed that StSWEET16b gene was involved in the interaction between tobacco and Phytophthora infestans. Furthermore, transient expression of StSWEET16b enhanced the resistance to P. infestans in tobacco, resulting in inhibiting the expansion of late blight disease spots. It inhibited the expansion of the disease spot of P. infestans and improved the disease resistance of the plant. The results suggesed that StSWEET16b protein played an important role in sugar transport and plant-pathogen interactions for resistance to late blight. This study provides a theoretical basis for improving potato quality and breeding of disease-resistant varieties.
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Received: 05 January 2021
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Corresponding Authors:
* zhouyun75@163.com
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