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| Changes of Microbial Communities in Recta and Cultured Water of Hyriopsis cumingii Under the Whole Artificial Breeding Mode |
| ZHOU Ni-Ni, CUI Ming-Hui, LI Zhen, QIAN Yun-Xia* |
| School of Marine Sciences, Ningbo University, Ningbo 315832, China |
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Abstract Environmental microbiota and intestinal microbiota play important roles in aquatic animals' health. This study investigated the changes of bacterial communities in recta and culture water of triangle sail mussel (Hyriopsis cumingii) fed by Monora phidiumcontortum and Cyclotella sp., respectively. The pH value, ammonia nitrogen (NH4+-N), nitrite nitrogen (NO2--N), nitrate nitrogen (NO3--N) and total phosphorus (TP) were measured on days 0, 7, 14 and 28, and the 16S rRNA of bacterial communities in the recta and water of the mussels were analyzed through high-throughput sequencing. In total, 44 bacterial phyla and 1 171 genera were identified in 21 water samples and 21 rectal samples. At the phylum level, the composition of bacterial communities in the recta and water was similar, both containing Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria and Verrucomicrobia, but the differences in relative abundance were significant. The bacterial composition at the genus level was significantly different and only two dominant genera were the same, Polynucleobacter and Reyranella, which belong to the same phylum of Proteobacteria. The results of redundancy analysis (RDA) showed that pH and NO2--N had the greatest effects on the water and rectal microbiota, respectively. KEGG function predicted that the microbial functions in rectal and water microbiota had a clear difference, but both were mostly related to energy production and biosynthesis. Principal coordinates analysis (PCoA) and permutational multivariate analysis of variance (PERMANOVA) combined analyses showed that 2 microalgae feeding had no significant effect on the structure of rectal and water microbiota; the water microbiota varied significantly at different time points (P<0.05); the rectal microbiota was relatively stable. This study showed that the 2 microalgae had no significant effect on the structure and function of the rectal and water bacterical communities of H. cumingii, but the hydrochemical parameters had an important influence on the microbiota, the results provide a theoretical basis for further optimization of the intensive culture.
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Received: 16 March 2021
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Corresponding Authors:
*qianyunxia@nbu.edu.cn
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