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| Effect of FaMYB10 Allele Nucleotide Polymorphism on Anthocyanin Accumulation in Tobacco (Nicotiana benthamiana) |
| WANG Hua1,2, DONG Jing1,2,3, YANG Yuan1,2,3, LI Mao-Fu1,2, SUN Pei1,2, FAN You-Wei1,2, WANG Gui-Xia1,2,3,*, JIN Wan-Mei1,2,* |
1 Beijing Academy of Forestry and Pomology Sciences, Beijing 100093, China;
2 Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture and Rural Affairs, Beijing 100093, China;
3 Beijing Engineering Research Center for Deciduous Fruit Trees, Beijing 100093, China |
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Abstract Anthocyanins are important pigments for strawberry (Fragaria × ananassa) fruit coloring, and FAMYB10 is a key transcription factor for anthocyanin synthesis. To investigate the role of nucleotide polymorphisms of FaMYB10 alleles in anthocyanin accumulation in tobacco (Nicotiana benthamiana), in this study, 3 alleles of FaMYB10 were isolated from the fruit by homologous cloning. FaMYB10a (GenBank No. MW478285) and FaMYB10b (GenBank No. MG456859) were identified from the red strawberry variety 'Sweet Charlie', and FaMYB10c (GenBank No. MG456860) was identified in the fruit of the white variety 'Snow White'. Sequence analysis showed that the open reading frame of FaMYB10a and FaMYB10b was 702 bp, encoding 233 amino acids. There was a single nucleotide polymorphism at 94 bp from start codon ATG, where FaMYB10a was base A, and FaMYB10b was base C. The open reading frame of FaMYB10c was 710 bp, and there was a 8 base insertions at 491 bp starting from ATG, which led to premature termination and the encoding of 179 amino acids. The bases insertion in the open reading frame of FaMYB10c resulted in differences in physicochemical properties and protein structure from the other 2 alleles. When FaMYB10a and FaMYB10b were overexpressed, the tobacco plants showed anthocyanin accumulation and a red phenotype. However, when FaMYB10c was overexpressed, the tobacco plants showed no anthocyanin accumulation. The above results indicated that the nucleotide polymorphisms of FaMYB10a and FaMYB10b did not affect the anthocyanin accumulation, while the nucleotide polymorphisms of FaMYB10c led to functional changes, and ultimately tobacco leaves could not accumulate anthocyanins. The present study provides reference for the functional research of other important genes, and also provides a reference for the study of plant traits and evolution.
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Received: 19 October 2020
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Corresponding Authors:
*wgxia1972@163.com;jwm0809@163.com
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