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| Polymorphisms and Haplotypes of MTNR1A Gene and Their Association with Litter Size in Sheep (Ovis aries) |
| WANG Ming-Yuan1,2, ZHU Meng-Ting1, SHAO Yan-Yan1, YANG Yong-Lin2, YU Qian2, Nijiati·Xiakaier3, ZHAO Zong-Sheng1,*, YANG Hua2,* |
1 College of Animal Science and Technology, Shihezi University, Xinjiang 832000, China;
2 State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China;
3 Shihezi Animal Health Supervision Institute of the 8th division of Xinjiang production and Construction Corps, Shihezi 832000, China |
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Abstract Melatonin receptor 1A (MTNR1A) is an important factor affecting the seasonal estrus and litter size in sheep (Ovis aries). To study the correlation of MTNR1A gene with litter size in Chinese Merino (Xinjiang Junken type) and Prolific Suffolk sheep, In this work, PCR, sequencing and restriction fragment length polymorphism (RFLP) techniques were used to detect the SNPs and genotypes in exon 2 of the MTNR1A gene. Linkage disequilibrium analysis was used to construct haplotypes. The association of MTNR1A gene polymorphisms and haplotypes with litter size was analyzed by general linear model (GLM) in two sheep groups. The results showed that there were 3 SNPs (G735A, G753A and C845A), each with three genotypes, were detected in two sheep breeds. SNP1 and SNP2 were synonymous mutations, SNP3 was a missense mutation with a single A to C substitution at nucleotide position 845 resulting in an alanine replacing an aspartic acid. The genotype distribution and allele frequencies of three SNPs in two sheep groups were in Hardy-Weinberg equilibrium and moderate polymorphisms. The associated analysis showed that G735A and G753A loci were extremely significantly associated with average litter size in sheep (P<0.01), the litter size of GG genotype was significantly higher than those of AG and AA genotypes (P<0.01) in two loci. The C845A loci was significantly associated with litter size in sheep (P<0.05), the litter size of CC genotype was significantly higher than those of CC and CG genotypes (P<0.05). Four haplotypes were constructed for three SNPs of MTNR1A gene. The different haplotypes were extremely significantly correlated with litter size in sheep (P<0.01), and the H1 haplotype was significantly correlated with higher average litter size than H4 (P<0.01). The results showed that MTNR1A gene was closely related to litter size in Chinese Merino (Xinjiang Junken type) and Prolific Suffolk sheep. H1 was the main haplotype of high litter size of sheep, G735A, G753A and C845A loci could be used as useful genetic markers in marker assisted selection for breeding of fecundity sheep.
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Received: 19 November 2020
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Corresponding Authors:
*zhaosongsh@shzu.edu.cn;yhxjcn@sina.com
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[1] 陈健楠, 张全伟, 王琪, 等.2020.MTNR1A和MTNR1b在牦牛不同组织中的表达及其在睾丸中的定位[J].兽类学报, 40(01): 64-70.
(Chen J N, Zhang Q W, Wang Q, et al.2020.Expression of MTNR1A and MTNR1b in different tissues of yak and their localization in testis[J].Acta Theriologica Sinica, 40(01): 64-70.)
[2] 程笃学.2015.绵、山羊MTNR1A基因的克隆测序及其与繁殖季节性的关联研究[D].硕士学位论文, 山西农业大学, 导师: 储明星.pp.43-47.
(Cheng D X.2015.Studies on cloning and sequencing of MTNR1A gene and its association with reproductive seasonality in sheep and goats[D].Thesis fo M.S., Shanxi Agricultural University, Supervisor: Chu M X, pp.43-47.)
[3] 储明星, 程笃学, 刘文忠, 等.2008.褪黑激素受体1A基因外显子2与小尾寒羊高繁殖力的关联[J].安徽农业大学学报, 2008(01): 1-4.
(Chu M X, Cheng D X, Liu W Z, et al.2008.Association between exon 2 of melatonin receptor lA gene and prolificacy in Small Tail Han sheep[J].Journal of Anhui Agricultural University, 2008(01): 1-4.)
[4] 宋倩, 王慧, 康丽, 等.2013.寿光鸡褪黑素受体基因(MTNR1A) 5'控区多态性与产蛋性状的关联分析[J].农业生物技术学报, 21(05): 579-587.
(Song Q, Wang H, Kang L, et al.2013.Polymorphisms of the 5' regulatory region of melatonin receptor gene (MTNR1A) and their associations with laying traits in Shouguang chickens(Gallus gallus)[J].Journal of Agricultural Biotechnology, 21(05): 579-587.)
[5] 王琼, 邵勇钢, 刘玲玲, 等.2013.策勒黑羊BMPR-IB、MTNR1A和PTGS2基因的RFLP分析[J].新疆农业科学, 50(07): 1330-1339.
(Wang Q, Shao Y G, Liu L L, et al.2013.RFLP analysis of BMPR-IB, MTNR1A and PTGS2 gene in Cele Black Sheep[J].Xinjiang Agricultural Sciences, 50(07): 1330-1339.)
[6] 王世银, 邓双义, 杨力伟, 等.2015.MTNR1A基因多态性与绵羊季节性繁殖的相关性分析[J].西北农业学报, 24(08): 23-30.
(Wang S Y, Deng S Y, Yang L W, et al, 2015.Polymorphism of melatonin receptor 1A (MTNR1A) and the relationship with seasonal reproduction of sheep (Ovis aries)[J].Acta Agriculturae Boreali-occidentalis Sinica, 24(08): 23-30.)
[7] 王忻.2009.非繁殖季节光照控制对蒙古羊发情排卵和褪黑激素受体表达的影响[D].硕士学位论文, 甘肃农业大学, 导师:张英杰.pp.40-44.
(Wang X.2009 Effect of light control on oestrus and ovulation and MTNR1A expression of Mongolia ewe in non-breeding season[D].Thesis fo M.S., Gansu Agricultural University, Supervisor: Zhang Y J, pp.40-44.)
[8] 王延莉, 金花子, 金海国.2018.褪黑素及相关基因调控哺乳动物季节性繁殖的研究进展[J].中国畜牧杂志, 54(12):30-35.
(Wang Y L, Jin H Z, Jin H G.2018.Research progress on melatonin and related genes regulating the seasonal reproduction of mammals[J].Chinese Journal of Animal Science, 54(12): 30-35.
[9] 杨永林, 杨华, 张云生, 等.2014.多胎萨福克羊新品系的选育[J].中国草食动物科学, (S1): 162-166.
(Yang Y L, Yang H, Zhang Y S, et al, 2014.Breeding of prolific suffolk new lines[J].China Herbivore Science, (S1): 162-166.)
[10] 章寒.2011.单倍型的分布估计和关联分析[D].博士学位论文, 中国科学技术大学, 导师: 杨亚宁.pp.1-6.
(Zhang H.2011.Haplotype estimation and association analysis[D].Thesis for Ph.D., University of Science and Technology of China, Supervisor: Yang Y N, pp.1-6.)
[11] 郑再明.2013.MTNR1A基因在内蒙古绒山羊下丘脑、垂体及卵巢中的表达[D].硕士学位论文, 内蒙古农业大学, 导师: 储明星.pp.32-33.
(Zheng Z M.2013.Expression of MTNR1A genes in hypothalamus, pituitary and ovary of Inner Mongolian Cashmere Goats[D].Thesis fo M.S., Inner Mongolia Agricultural University, Supervisor: Chu M X, pp.32-33.)
[12] Ahmad T, Ganai T A S, Pandey A K, et al.2013.Phylogenetic analysis of exon Ⅱ of melatonin receptor lA (MTNR1A) gene in merino cross EWES in Kashmir[J].Indian Journal of Animal Research, 47(1): 66-69.
[13] Akey J, Jin L, Xiong M.2001.Haplotypes vs single marker linkage disequilibrium tests: What do we gain?[J].European Journal of Human Genetics, 9(4):291-300.
[14] Carcangiu V, Luridiana S, Vacca G M, et al.2011.A polymorphism at the melatonin receptor 1A (MTNR1A) gene in Sarda ewes affects fertility after AI in the spring[J].Reproduction Fertility and Development, 23(2): 376-80.
[15] Carcangiu V, Mura M C, Vacca G M, et al.2009.Polymorphism of the melatonin receptor MT1 gene and its relationship with seasonal reproductive activity in the Sarda sheep breed[J].Animal Reproduction Science, 116(1):65-72.
[16] Clark A.G.1990.Inference of haplotypes from PCR-amplified samples of diploid populations[J].Molecular Biology & Evolution, 7(2): 111-122.
[17] Excoffier L, Slatkin M.1995.Maximum-likelihood estimation of molecular haplotype frequencies in a diploid population[J].Molecular Biology and Evolution.12(5): 921-927.
[18] He X, Zhang Z, Liu Q, et al.2019.Polymorphisms of the melatonin receptor 1A gene that affects the reproductive seasonality and litter size in Small Tail Han sheep[J].Reproduction in Domestic Animals, 54(10): 1400-1410.
[19] Hernandez X, Bodin L, Chesneau D, et al.2005.Relationship between MT1 melatonin receptor gene polymorphism and seasonal physiological responses in Ile-de-France ewes[J].Reproduction Nutrition Development, 45(2): 151-62.
[20] Kimchisarfaty C, Oh J M, Kim I W, et al.2007.A "silent" polymorphism in the MDR1 gene changes substrate specificity[J].Science, 315: 525-528.
[21] Luridiana S, Mura M C, Daga C, et al.2015.The relationship between melatonin receptor 1A gene (MTNR1A) polymorphism and reproductive performance in Sarda breed sheep[J].Livestock Science, 171: 78-83.
[22] Malpaux B, Thiéry J C, Chemineau P.1999.Melatonin and the seasonal control of reproduction[J].Animal, 39(3): 355-66.
[23] Martínez-Royo A, Lahoz B, Alabart J L, et al.2012.Characterisation of the melatonin receptor 1A (MTNR1A) gene in the Rasa Aragonesa sheep breed: Association with reproductive seasonality[J].Animal Reproduction Science, 133(3-4): 169-175.
[24] Mura M C, Luridiana S, Pulinas L, et al.2018.Reproductive response to male joining with ewes with different allelic variants of the MTNR1A gene[J].Animal Reproduction Science, 200: 60-66.
[25] Mura M C, Luridiana S.Bodano S, et al.2014.Influence of melatonin receptor 1A gene polymorphisms on seasonal reproduction in Sarda ewes with different body condition scores and ages[J].Animal Reproduction Science, 149(3-4): 173-177.
[26] Pelletier J, Bodin L, Hanocq E, et al.2000.Association between expression of reproductive seasonality and alleles of the gene for Mel1a receptor in the ewe1[J].Biology of Reproduction, 62(4): 1096-1101.
[27] Perry Barrett, Shaun Conway, Ralf Jockers, et al.1997.Cloning and functional analysis of a polymorphic variant of the ovine Mel 1a melatonin receptor[J].Biochimica et biophysica acta, 1356(3): 299-307.
[28] Saxena V K, Jha B K, Meena A S, et al.2015.Assessment of genetic variability in the coding sequence of melatonin receptor gene (MTNR1A) in Tropical Arid Sheep breeds of India[J].Biochemical Genetics, 50(3): 517-21.
[29] Starič J, Farci F, Luridiana S, et al.2020.Reproductive performance in three Slovenian sheep breeds with different alleles for the MTNR1A gene[J].Animal Reproduction Science, 216: 106352.
[30] Valentino D, Boro M.2015.Association of the MTNR1A gene polymorphisms and seasonal reproduction in sheep[J].Agriculture, 21(2): 73-77.
[31] Vandeputte M, Dupont-Nivet M, Chavanne H, et al.2007.A polygenic hypothesis for sex determination in the European sea bass Dicentrarchus labrax[J].Genetics, 176(2): 1049-1057. |
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