|
|
Functional Analysis of Verticillium dahliae Asp f2-like Protein (VDAL) in Fungal Pathogen Resistance and Yield Improvement in Oryza sativa |
WANG Yan-Jun1,*, FU Yu-Hua2,*, ZHONG Ying-Li3, LI Hong-Liang4, REN Hai-Yang4, DONG Guo-Jun5, REN Zuo-Hua6, WANG Kai1, GAO Yuan-Hui1, GONG Zhi-Zhong1, YUAN Xiao-Hui2,**, QI Jun-Sheng1,** |
1 State Key Laboratory of Plant Physiology and Biochemistry/Department of Plant Science, College of Biological Sciences, China Agricultural University, Beijing 100193, China; 2 School of Computer Science and Technology, Wuhan University of Technology, Wuhan 430070, China; 3 College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha 410128, China; 4 Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157041, China; 5 China Rice Research Institute, Hangzhou 311400, China; 6 College of Plant Protection, Hunan Agriculture University, Changsha 410128, China |
|
|
Abstract Peptides secreted by pathogens serve as elicitors of plant defense responses. Verticillium dahliae Aspf2-like protein (VDAL) is secretory protein of V. dahliae and belongs to Aspergillus fumigatus f2-like zinc-binding protein. The VDAL146 protein in V. dahliae Vd146 strain of cotton (Gossypium spp.) was made into dry powder by engineering bacteria fermentation, and its diluent was sprayed on the leaves, which could activate the immune response of plants such as rice (Oryza sativa) and promote the growth and development of plants. In present study, VDAL146 (MT974503) was isolated and characterized, and sprayed twice before and after rice break stage. The results showed enhanced resistance to rice sheath blight, as well as improved yield by 14.5% via increasing both the number of grains per panicle and thousand grain weight. At the same time, VDAL146 did not show direct fungicidal activity. In addition, transcriptome analysis indicated that VDAL146 treatment resulted in upregulation of 322 genes involved in carbohydrate metabolism, organophosphate and glucan biosynthesis, and ATP binding. In conclusion, VDAL146 holds the potential to be developed in fungicide and biostimulant to enhance disease resistance and growth promotion in rice crop.
|
Received: 25 May 2020
|
|
Corresponding Authors:
**yuanxiaohui@whut.edu.cn, junshqi@cau.edu.cn
|
About author:: * The authors who contribute equally |
|
|
|
[1] 杜蕙, 蒋晶晶. 2020. 生物农药与化学杀菌剂对葡萄霜霉病的联合作用效果[J]. 甘肃农业科技, (01): 25-29. (Du H, Jiang J J. 2020. Combined effects of bio-pesticides combined with chemical fungicides on grape downy mildew[J]. Gansu Agricultural Science and Technology, (01): 25-29.) [2] 贾秀领, 张经廷, 马贞玉, 等. 2016. 植物免疫诱抗剂"阿泰灵"为作物生长保驾护航[J]. 现代农村科技, (15): 25. (Jia X L, Zhang J T, Ma Z Y, et al. 2016. The plant immune inducer 'ATaiLing' escorts the crop growth[J]. Modern Agricultural Science and Technology, (15): 25.) [3] 张桂娟, 林雪, 陈虹, 等. 2020. 生物刺激素在春季花椰菜上的应用研究[J]. 蔬菜, (5): 26-31. (Zhang G J, Lin X, Chen H, et al. 2020. Study on the application of biostimulator in spring cauliflower[J]. Vegetables, (5): 26-31.) [4] 张强, 刘祥臣, 余贵龙, 等. 2019. 不同浓度阿泰灵对再生稻两优6326秧苗素质和纹枯病抗性及产量的影响[J]. 江苏农业科学, 47(15): 130-133. (Zhang Q, Liu X C, Yu G L, et al.2019. Impacts of different concentrations of plant immune inducer ATaiLing on seeding quality, resistence to sheath blight and yield of ratoon rice 'Liangyou 6326'[J]. Jiangsu Agricultural Sciences, 47(15): 130-133.) [5] 周锡跃, 徐春春, 李凤博, 等. 2010. 世界水稻产业发展现状、趋势及对我国的启示[J]. 农业现代化研究, 31(05): 525-528. (Zhou X Y, Xu C C, Li F B, et al.2010. Status quo and trends of world's rice industry development and its enlightenment to China[J]. Research of Agricultural Modernization, 31(05): 525-528.) [6] Amich J, Vicentefranqueira R, Leal F, et al.2010. Aspergillus fumigatus survival in alkaline and extreme zinc-limiting environments relies on the induction of a zinc homeostasis system encoded by the zrfC and aspf2 genes[J]. Eukaryot Cell, 9(3): 424-437. [7] Anil K, Podile A R.2012. HarpinPss-mediated enhancement in growth and biological control of late leaf spot in groundnut by a chlorothalonil-tolerant Bacillus thuringiensis SFC24[J]. Microbiological Research, 167(4): 194-198. [8] Bhat R G, Subbarao K V.1999. Host range specificity in Verticillium dahliae[J]. Phytopathology, 89(12): 1218-1225. [9] Bhuvaneswari V, Raju S K.2012. Efficacy of new combination fungicide against rice sheath blight caused by Rhizoctonia solani (Kuhn)[J]. Journal of Rice Research, 5(1): 2. [10] Choi M S, Kim W, Lee C, et al.2013. Harpins, multifunctional proteins secreted by gram-negative plant-pathogenic bacteria[J]. Molecular Plant-Microbe Interactions, 26(10): 1115-1122. [11] Citiulo F, Jacobsen I D, Miramon P, et al.2012. Candida albicans scavenges host zinc via Pra1 during endothelial invasion[J]. PLOS Pathogens, 8(6): e1002777. [12] Collmer A, Badel J L, Charkowski A O, et al.2000. Pseudomonas syringae Hrp typeⅢsecretion system and effector proteins[J]. Proceedings of the National Academy of Sciences of the USA, 97(16): 8770-8777. [13] de Jonge R, van Esse H P, Maruthachalam K, et al.2012. Tomato immune receptor Ve1 recognizes effector of multiple fungal pathogens uncovered by genome and RNA sequencing[J]. Proceedings of the National Academy of Sciences of the USA, 109(13): 5110-5115. [14] de Torres Zabala M, Littlejohn G, Jayaraman S, et al.2015. Chloroplasts play a central role in plant defence and are targeted by pathogen effectors[J]. Nature Plants, 1(6): 15074. [15] Delprato M L, Krapp A R, Carrillo N.2015. Green light to plant responses to pathogens: The role of chloroplast light-dependent signaling in biotic stress[J]. Photochemistry and Photobiology, 91(5): 1004-1011. [16] Dewen Q, Yijie D, Yi Z, et al.2017. Plant immunity inducer development and application[J]. Molecular Plant-Microbe Interactions, 30(5): 355-360. [17] Gilardoni P A, Schuck S, Jüngling R, et al.2010. Super SAGE analysis of the Nicotiana attenuata transcriptome after fatty acid-amino acid elicitation (FAC): Identification of early mediators of insect responses[J]. BMC Plant Biology, 10: 66. [18] Grichar W, Jaks A, Besler B A.2005. Response of peanuts (Arachis hypogaea) to weather-based fungicide advisory sprays[J]. Crop Protection, 24(4): 349-354. [19] Grisham J.2000. Protein biopesticide may be next wave in pest control[J]. Nature Biotechnology, 18(6): 595-596. [20] Groth D.2008. Effects of cultivar resistance and single fungicide application on rice sheath blight, yield, and quality[J]. Crop Protection, 27(7): 1125-1130. [21] Inderbitzin P, Subbarao K V.2014. Verticillium systematics and evolution: How confusion impedes Verticillium wilt management and how to resolve it[J]. Phytopathology, 104(6): 564-574. [22] Jia L, Yan W, Zhu C, et al.2012. Allelic analysis of sheath blight resistance with association mapping in rice[J]. PLOS ONE, 7(3): e32703. [23] Jiao Y, Xu W, Duan D, et a.2016. A stilbene synthase allele from a Chinese wild grapevine confers resistance to powdery mildew by recruiting salicylic acid signalling for efficient defence[J]. Journal of Experimental Botany, 67(19): 5841-5856. [24] Jones J.2001. Harpin[J]. Pesticide Outlook, 12(4): 134-135. [25] Karmakar S, Molla K A, Das K, et al.2017. Dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice[J]. Scientific Reports, 7(1): 7900. [26] Kearney J.2010. Food consumption trends and drivers[J]. Philosophical Transactions of the Royal Society B: Biological Sciences, 365(1554): 2793-2807. [27] Kim J G, Park B K, Yoo C H, et al.2003. Characterization of the Xanthomonas axonopodis pv. glycines Hrp pathogenicity island[J]. Journal of Bacteriology, 185(10): 3155-3166. [28] Liu H, Wang Y, Zhou X, et al.2016. Overexpression of a harpin-encoding gene popW from Ralstonia solanacearum primed antioxidant defenses with enhanced drought tolerance in tobacco plants[J]. Plant Cell Reports, 35(6): 1333-1344. [29] Modrof J, Becker S, Muhlberger E.2003. Ebola virus transcription activator VP30 is a zinc-binding protein[J]. Journal of Virology, 77(5): 3334-3338. [30] Nagarajkumar M, Bhaskaran R, Velazhahan R.2004. Involvement of secondary metabolites and extracellular lytic enzymes produced by Pseudomonas fluorescens in inhibition of Rhizoctonia solani, the rice sheath blight pathogen[J]. Microbiological Research, 159(1): 73-81. [31] Rafalski J A.2002. Plant genomics: Present state and a perspective on future developments[J]. Briefings in Functional Genomics & Proteomics, 1(1): 80-94. [32] Savary S, Teng P S, Willocquet L, et al.2006. Quantification and modeling of crop losses: A review of purposes[J]. Annual Review of Phytopathology, 44: 89-112. [33] Savary S, Willocquet L, Elazegui F A, et al.2000. Rice pest constraints in tropical Asia: Quantification of yield losses due to rice pests in a range of production situations[J]. Plant Disease, 84(3): 357-369. [34] Schuck S, Camehl I, Gilardoni P A, et al.2012. HSPRO controls early Nicotiana attenuata seedling growth during interaction with the fungus Piriformospora indica[J]. Plant Physiology, 160(2): 929-943. [35] Singh S K, Shukla V, Singh H, et al.2004. Current status and impact of sheath blight in rice (Oryza sativa L.)-a review[J]. Agricultural Reviews, 25(4): 289-297. [36] Sheng S Y, Zhou Q, Qiu D W, et al.2017. Effects and mechanism of disease resistance and yield improvement induced by plant immune protein preparation ATailing in wheat[J]. Chinese Journal of Biological Control, 33(2): 213-218. [37] Wang W, Liu N, Gao C, et al.2019. The Pseudomonas Syringae effector AvrPtoB associates with and ubiquitinates Arabidopsis exocyst subunit EXO70B1[J]. Frontiers in Plant Science, 10:1027. [38] Wang J Y, Cai Y, Gou J Y, et al.2004. VdNEP, an elicitor from Verticillium dahliae, induces cotton plant wilting[J]. Applied and Environmental Microbiology, 70(8): 4989-4995. [39] Wei Z M, Laby R J, Zumoff C H, et al.1992. Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovora[J]. Science, 257(5066): 85-88. [40] Yu J, Hu S, Wang J, et al.2002. A draft sequence of the rice genome (Oryza sativa L. ssp. indica)[J]. Science, 296(5565): 79-92. [41] Zhang D D, Wang J, Wang D, et al.2019. Population genomics demystifies the defoliation phenotype in the plant pathogen Verticillium dahliae[J]. New Phytologist, 222(2): 1012-1029. [42] Zhang W, Yang X, Qiu D, et al.2010. Activator protein PeaT1 induced systemic resistance to Tobacco mosaic virus in tobacco[J]. Acta Phytopathologica Sinica, 40(3): 290-299. |
[1] |
JIANG Bing-Bing, HUANG Man-Man, BAI Tian, ZENG Tao, CHEN Li, SHEN Jun-Da, TAO Zheng-Rong, ZHOU Wei, LIU Guo-Fa, LU Li-Zhi, LI Hai-Ying. Transcriptome Sequencing Analysis of Differentially Expressed Genes Associated with Differential Color Feather Bulbs in Ducks (Anas platyrhynchos)[J]. 农业生物技术学报, 2020, 28(9): 1623-1634. |
|
|
|
|