大丽轮枝孢(<em>Verticillium dahliae</em>) Asp f2类蛋白(VDAL)在水稻(<em>Oryza sativa</em>)中抵御真菌病害及提高产量的功能分析

doi:10.3969/j.issn.1674-7968.2020.12.001

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Abstract Peptides secreted by pathogens serve as elicitors of plant defense responses. Verticillium dahliae Aspf2-like protein (VDAL) is secretory protein of V. dahliae and belongs to Aspergillus fumigatus f2-like zinc-binding protein. The VDAL146 protein in V. dahliae Vd146 strain of cotton (Gossypium spp.) was made into dry powder by engineering bacteria fermentation, and its diluent was sprayed on the leaves, which could activate the immune response of plants such as rice (Oryza sativa) and promote the growth and development of plants. In present study, VDAL146 (MT974503) was isolated and characterized, and sprayed twice before and after rice break stage. The results showed enhanced resistance to rice sheath blight, as well as improved yield by 14.5% via increasing both the number of grains per panicle and thousand grain weight. At the same time, VDAL146 did not show direct fungicidal activity. In addition, transcriptome analysis indicated that VDAL146 treatment resulted in upregulation of 322 genes involved in carbohydrate metabolism, organophosphate and glucan biosynthesis, and ATP binding. In conclusion, VDAL146 holds the potential to be developed in fungicide and biostimulant to enhance disease resistance and growth promotion in rice crop.

Key words： Verticillium dahliae Aspf2-like protein (VDAL) Biostimulant Resistance Yield RNA-seq Rice

Received: 25 May 2020

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S482

Corresponding Authors: **yuanxiaohui@whut.edu.cn, junshqi@cau.edu.cn

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	WANG Yan-Jun
	FU Yu-Hua
	ZHONG Ying-Li
	LI Hong-Liang
	REN Hai-Yang
	DONG Guo-Jun
	REN Zuo-Hua
	WANG Kai
	GAO Yuan-Hui
	GONG Zhi-Zhong
	YUAN Xiao-Hui
	QI Jun-Sheng

Cite this article:

WANG Yan-Jun,FU Yu-Hua,ZHONG Ying-Li, et al. Functional Analysis of Verticillium dahliae Asp f2-like Protein (VDAL) in Fungal Pathogen Resistance and Yield Improvement in Oryza sativa[J]. 农业生物技术学报, 2020, 28(12): 2091-2107.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2020.12.001 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2020/V28/I12/2091

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