Abstract Jasmonic acid substances,as endogenous regulatory hormones in plants,play important roles in plant growth and responding to stress. Jasmonic acid carboxyl methyltransferase (JMT) is a key enzyme that catalyzes synthesizing methyl jasmonate (MeJA) from jasmonate. It controls the transformation of jasmonate in plants and affects plants resistance to biological and abiotic stress. Based on sugarcane (Saccharum spp. hybrids) transcriptome database,a full-length of sugarcane jasmonate methyltransferase ScJMT (GenBank No.MK784565) was cloned and analyzed. The expression levels of ScJMT in different organs and expression profiles under different stresses were detected by qRT-PCR. Bioinformatics results showed that the open reading frame (ORF) of ScJMT gene was 1 110 bp encoding 369 aa. The isoelectric point,instability coefficient,and average hydrophobicity value of the ScJMT protein were 5.28,56.76,-0.135,respectively. The ScJMT was an unstable acid hydrophilic protein by prediction. The α-helix structure and random curl constituted its secondary structure and tertiary structure,and it also contained the methylation_7 conserved domain. Through phylogenetic tree analysis showed ScJMT was on the same branch as SvJMT from Sorghum vulgare and ZmJMT from Zea mays. In addition,the spatial expression level of ScJMT was detected and expression profiles under different stress were also determined. There were no significant differences among 3 sugarcane organs,and ScJMT was increased in abscisic acid (ABA),drought (PEG6000),high salinity treatments and oriental armyworm Mythirnna separata feeding stress,while treatments of MeJA and Acidovorax avenae subsp. avenae showed no effect on ScJMT expression. The results make foundations for further research on the role of this gene,and also provide gene resources for the cultivation of new resistant sugarcane varieties.
[1] 安汶铠,严晓红,包秋娟,等.2017.沉默茉莉酸甲基转移酶(GhJMT)基因对棉花幼苗生理指标的影响[J].新疆农业科学,54(02):213-222. (An W K,Yan X H,Bao Q J,Zhang F C,et al.2017.Effect of silencing jasmonate methyltransferase (GhJMT) gene on physiological indexes of Gossypium hirsutum seedlings[J].Xinjiang Agricultural Sciences,54(02):213-222.) [2] 白辉杨.2019.紫苏防御素和茉莉酸羟基甲基转移酶基因的克隆及功能研究[D].硕士学位论文,重庆师范大学,导师:张涛,pp.1-58. (Bai H Y.2019.Cloning and functional study of the genes ofPerilla frutescensdefensin and jasmonate hydroxymethyl transferase[D].Thesis for M.S.,Chongqing Normal University,Supervisor:Zhang T,pp.1-58.) [3] 高磊.2016.桃响应LASIODIPLODIA THEOBROMAE侵染的基因表达变化及桃流胶病菌的侵染特性研究[D].博士学位论文,华中农业大学,导师:李国怀,pp.37-39. (Gao L.2016.Gene expression Changes in peach in response to LASIODIPLODIA THEOBROMAE infection and infection characteristics of Botryosphaeria spp.[D].Thesis for Ph.D.,Huazhong Agricultural University,Supervisor:Li G H.pp.37-39.) [4] 韩秀.2012.水稻抗虫相关基因OsSABATH12和OsJMT1的功能分析[D].硕士学位论文,浙江大学,导师:娄永根,pp.43-44. (Han X.2012.Functional analysis ofOsSABATH12andOsJMT1in rice[D].Thesis for M.S.,Zhejiang University,Supervisor:Lou Y G.pp.43-44.) [5] 黄铭坤,何杰玲,范燕萍.2011.植物SABATH甲基转移酶研究进展[J].植物生理学报,47(09):840-846. (Huang M K,He J L,Fan Y P.2011.Advances in research on plant SABATH methyltransferase[J].Plant Physiology Journal,47(09):840-846.) [6] 姜礅,王杰,姜虹,等.2016.茉莉酸甲酯局部诱导长白落叶松对舞毒蛾生长发育的影响[J].北京林业大学学报,38(06):67-71. (Jiang D,Wang J,Jiang H,et al.2016.Effects of locally inducingLarix olgensisusing exogenous methyl jasmonate on the growth and development ofLymantria dispar[J].Journal of Beijing Forestry University,38(06):67-71.) [7] 蒋科技,皮妍,侯嵘,等.2010.植物内源茉莉酸类物质的生物合成途径及其生物学意义[J].植物学报,45(02):137-148. (Jiang K J,Pi Y,Hou R,et al.2010.Biosynthesis of plant endogenous jasmonic acid and its biological significance[J].Journal of Integrative Plant Biology,45(02):137-148.) [8] 甘蔗茉莉酸甲基转移酶基因ScJMT的克隆与表达分析 (Cloning and Expression Analysis of Jasmonic Acid Carboxyl Methyltransferase Gene(JMT) in Sugarcane) [9] 矫健,聂雁蓉,陈伟忠,等.2019.近期国内外食糖产业形势分析与展望[J].农业展望,15(01):50-53+60. (Jiao J,Nie Y R,Chen W Z,et al.2019.Recent situation analysis and prospect of domestic and foreign sugar industry[J].Agricultural Outlook,15(01):50-53+60.) [10] 井维霞.2017.棉花抗虫相关基因GhJMTs的克隆及功能鉴定[D].硕士学位论文,山东农业大学,导师:曲爱军,pp.36-38. (Jing W X.2017.Cloning and functional identification ofGossypium hirsutumanti-insect related gene GhJMTs[D].Thesis for M.S.,Shandong Agricultural University,Supervisor:Qu A J.pp.36-38.) [11] 李晓燕.2017.甘蔗赤条病菌的分离鉴定与遗传多样性研究[D].硕士学位论文,福建农林大学,导师:高三基,pp.12-13. (Li X Y.2017.Isolation,identification and genetic diversity ofAcidovorax avenaecauseing red stripe of sugarcane[D].Thesis for M.S.,Fujian Agriculture and Forestry University,Supervisor:Gao S J,pp.12-13.) [12] 李清清,李大鹏,李德全.2010.茉莉酸和茉莉酸甲酯生物合成及其调控机制[J].生物技术通报,(01):53-57+62. (Li Q Q,Li D P,Li D Q.2010.Biosynthesis of jasmonic acid and methyl jasmonate and its regulatory mechanism[J].Biotechnology Bulletin,(01):53-57+62.) [13] 陆续,江伟民,唐克轩.2011.茉莉酸类物质在植物次生代谢调控方面的研究进展[J].上海交通大学学报(农业科学版),29(06):87-91. (Lu X,Jiang W M,Tang K X.2011.Research progress of jasmonic acids in the regulation of plant secondary metabolism[J].Journal of Shanghai Jiaotong University (Agricultural science edition),29(06):87-91.) [14] 马广民,刘守柱,杜学林,等.2018.外源茉莉酸甲酯诱导的棉花防御对棉蚜及斜纹夜蛾发育的影响[J].应用昆虫学报,55(03):399-406. (Ma G M,Liu S Z,Du X L,et al.2018.Effect of exogenous MeJA treatment of cotton on the development of Aphis gossypii (Hemiptera:Aphididae) andSpodoptera litura(Lepidptera:Noctuidae)[J].Journal of Applied Entomology,55(03):399-406.) [15] 阙友雄,许莉萍,徐景升,等.2009.甘蔗基因表达定量PCR分析中内参基因的选择[J].热带作物学报,030(003):274-278. (Que Y X,Xu L P,Xu J S,et al.2009.Selection of control genes in real-time qPCR analysis of gene expression in sugarcane[J].Chinese Journal of Tropical Crops,030(003):274-278.) [16] 孙凯文.2019.植物激素脱落酸对茉莉酸介导的防御反应的调控[D].硕士学位论文,南京师范大学,导师:朱自强,pp.28-32. (Sun K W.2019.The regulation of plant hormone abscisic acid on the defense response mediated by jasmonic acid[D].Thesis for M.S.,Nanjing Normal University,Supervisor:Zhu Z Q,pp.28-32.) [17] 吴莹莹,黄凤宽,韦素美,等.2008.茉莉酸甲酯诱导水稻后对褐飞虱的抗性研究[J].广西农业科学,(04):474-477. (Wu Y Y,Huang F K,Wei S M,et al.2008.Resistance of rice induced by Methyl jasmonate to rice brown planthopper[J].Guangxi Agricultural Sciences,(04):474-477.) [18] 徐涛,周强,陈威,等.2003.茉莉酸信号传导途径参与了水稻的虫害诱导防御过程[J].科学通报,(13):1442-1446. (Xu T,Zhou Q,Chen W,et al.2003.The jasmonic acid signaling pathway is involved in the pest induction and defense process of rice[J].Chinese Science Bulletin,(13):1442-1446.) [19] 徐伟,严善春.2005.茉莉酸在植物诱导防御中的作用[J].生态学报,(08):2074-2082. (Xu W,Yan S C.2005.The roles of jasmonic acid in plant induced defense[J].Acta Ecologica Sinica,(08):2074-2082). [20] 杨艺,张富春.2015.棉花茉莉酸羧基甲基转移酶基因克隆及其表达分析[J].西北农业学报,24(08):50-56. (Yang Y,Zhang F C.2015.Cloning and expression analysis ofGossypium hirsutumjasmonate carboxymethyl transferase gene[J].Acta Agriculturae Boreali-occidentalis Sinica,24(08):50-56.) [21] Avanci N C,Luche D D,Goldman G H,et al.2010.Jasmonates are phytohormones with multiple functions,including plant defense and reproduction[J].Genetics and Molecular Research,9(1):484-505. [22] Baldwin I T,Zhang Z P,Diab N,et al.1997.Quantification,correlations and manipulations of wound-induced changes in jasmonic acid and nicotine inNicotiana sylvestris[J].Planta,201(4):397-404. [23] Choi Y D (KR),Cheong J J (KR),Lee J S (KR),et al.2003.Genes for s-adenosyl l-methionine:Jasmonic acid carboxyl methyltransferase and a method for the development of pathogen-and stress-resistant plants using the genes[P].United States,US2003064895. [24] Dammann C,Rojo E,Jose J,et al.1997.Abscisic acid and jasmonic acid activate wound‐inducible genes in potato through separate,organ‐specific signal transduction pathways[J].Plant Journal,11(4):773-782. [25] Hui L,Wu Q B,Guo J L,et al.2014.Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative RT-PCR[J].PLOS ONE,9(5):e94767. [26] Meur G,Shukla P,Dutta G A,et al.2015.Characterization ofBrassica juncea-Alternaria brassicicolainteraction and jasmonic acid carboxyl methyl transferase expression[J].Plant Gene,3:1-10. [27] Sohn H B,Lee H Y,Seo J S,et al.2011.Overexpression of jasmonic acid carboxyl methyltransferase increases tuber yield and size in transgenic potato[J].Plant Biotechnology Reports,5(1):27-34. [28] Preuß A,Augustin C,Figueroa C R,et al.2014.Expression of a functional jasmonic acid carboxyl methyltransferase is negatively correlated with strawberry fruit development[J].Journal of Plant Physiology,171(15):1315-1324. [29] Qi J F,Li J C,Han X,et al.2016.Jasmonic acid carboxyl methyltransferase regulates development and herbivory-induced defense response in rice[J].Journal of Integrative Plant Biology,58(06):564-576. [30] Robert A C,John E M.Jasmonic acid distribution and action in plants:regulation during development and response to biotic and abiotic stress[J].Proceedings of the National Academy of Sciences of the USA,1995,92(10):4114-4119. [31] Sarafat A M,Baek K H.2020.Jasmonic acid signaling pathway in response to abiotic stresses in plants[J].International Journal of Molecular Sciences,21(2):621. [32] Song M S,Kim D G,Lee S H.2005.Isolation and characterization of a jasmonic acid carboxyl methyltransferase gene from hot pepper (Capsicum annuumL.)[J].Journal of Plant Biology,48(3):292-297. [33] Seo H S,Song J T,Cheong J J,et al.2001.Jasmonic acid carboxyl methyltransferase:A key enzyme for jasmonate-regulated plant responses[J].Proceedings of the National Academy of Sciences of the USA,98(8),pp.4788-4793. [34] Truman W,Bennettt M,Kubigsteltig I,et al.Arabidopsis systemic immunity uses conserved defense signaling pathways and is mediated by jasmonates[J].Proceedings of the National Academy of ences of the USA,2007,104(3):1075-1080. [35] Woo M C,Byung W M .2012.Bacterial pathogen protection in transgenic Chinese cabbage by expression of jasmonic acid carboxyl methyltransferase gene[J].Journal of Plant Biotechnology,39(2):99-105. [36] Xu Q,Wang S T,Hong H Z,et al.2019.Transcriptomic profiling of the flower scent biosynthesis pathway ofCymbidium faberiRolfe and functional characterization of its jasmonic acid carboxyl methyltransferase gene[J],20(125):2-14.
