玉米大斑病菌转录因子<em>StHTF2</em>基因的克隆及其在侵染过程中的表达分析

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Abstract Homeobox transcription factor (HTF) is a type of transcriptional regulatory factors with conservative structure and function. These genes can affect the growth and development and morphogenesis of eukaryotic organisms by participating in regulating the expression of functional genes. StHTF2 is a member of the HTF transcription factor family of Setosphaeria turcica playing an important role during the infection process. In this study,StHTF2 gene (GenBank No. XP_008029609.1) was cloned. The analysis results of gene sequence and its encoded protein characteristics showed that StHTF2 gene has 1 747 bp in total length and contained an open reading frame of 1 641 bp,which encoded 546 amino acids. Moreover,StHtf2 contained a highly conserved homologous domain HOX,which was conserved in eukaryotic organisms. The spatial structure formed from this domain including 3 helix regions could bind to a specific DNA motif to perform the function of a transcription factor. The expression and identification results of the StHtf2-His fusion protein showed that the fusion protein can be expressed in the Escherichia coli system,and the expression efficiency was higher at an induction temperature of 42 ℃,an induction pH of 7,and an induction time of 6 h. Furthermore,analysis of StHTF2 expression level during infection showed that compared with the conidium stage of the strain,the expression level of StHTF2 gene in susceptible maize (Zea mays) 'B73' and resistant maize 'B73Ht' were increased by 4.5 and 2 times,respectively,when the pathogens were infected for 24 h on the leaves of maize,which predicted that StHTF2 gene was involved in the infection process of pathogens and its expression was affected by the host. The above research results will not only provide a prerequisite for preparing StHtf2 protein antibodies and further studying the interaction of StHtf2 protein with other proteins but also create the prerequisite for revealing the molecular mechanism when the pathogen infect the hosts.

Key words： Setosphaeria turcica StHTF2 gene Gene cloning Prokaryotic expression Infection expression

Received: 14 February 2020

ZTFLH:

S435.131

Corresponding Authors: **gushouqin@126.com;hanjmnd@163.com

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2020/V28/I11/1913

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