小麦芒抑制基因Hd的定位和候选基因分析

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (2535 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Wheat (Triticum aestivum) awn,an important agronomic character of wheat controlled by many genes,plays an important role in resisting insect pests,seed reproduction and increasing wheat yield. CASL4AL is a chromosome arm substitution line (CASL) of wild emmer (T. dicoccoides,TTD) accession TTD140 in the genetic background of common wheat variety Chinese Spring (T. aestivum cv. Chinese Spring,CS),the long arm of chromosome 4A of CASL4AL is replaced by TTD140,showing long awn. In this study,a F2 population with 134 lines was constructed by using CASL4AL with long awn and the line of 442 with hook awn (derived from the cross of CASL4AL×CS,hooded awn as CS). The SSR molecular markers were used for QTL mapping for the awn length of wheat by ICIM (inclusive composite interval mapping) method. A QTL with LOD value of 30.71 was detected on the chromosome 4AS related to awn length,explaining 82.63% of the phenotypic variation. In this mapping interval,the annotation of the variants (SNP and InDel) for the CASL4AL resequencing data were conducted according to reference wheat genome,combined with the differential analysis of transcriptome sequencing,five differentially expressed genes with sense variation were obtained,among which,TraesCS4A02G078700 translates MADS-box transcription factor 1,which involved in the differentiation and proliferation of the meristem of lemma and palea,and probably involved in regulating the growth of wheat awn. This study provide reference for the screening of fine mapping materials and fine mapping of awn gene Hd (Hooded).

Key words： Chromosome arm substitution line Awn Hooded gene QTL mapping Resequencing Transcriptome

Received: 17 January 2020

ZTFLH:

S512.1

Corresponding Authors: *huxin98@foxmail.com

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	YANG Si-Qing
	HU Le-Jia
	HU Xin
	RONG Jun-Kang

Cite this article:

YANG Si-Qing,HU Le-Jia,HU Xin, et al. Mapping and Candidate Gene Analysis of Awn Inhibition Gene Hd in Wheat (Triticum aestivum)[J]. 农业生物技术学报, 2020, 28(11): 1901-1912.

URL:

http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2020/V28/I11/1901

[1] 杜斌,崔法,王洪刚,等.2010.小麦芒长抑制基因B1近等基因系的鉴定及遗传分析[J].分子植物育种 8(02):259-264.
(Du B,Cui F,Wang H G,et al.2010.Identification and genetic analysis of awn length inhibitory gene B1 near isogenic lines in wheat[J].Molecular Plant Breeding 8(02):259-264.)
[2] 高亚男.2015.小麦芒长近等基因系的遗传分析与转录组研究[D].硕士学位论文,山东农业大学,导师:李兴峰,pp.1-89.
(Gao Y N.2015.Genetic analysis and transcriptome study of awn length near isogenic lines in wheat[D].Master's thesis,Shandong Agricultural University,tutor:Li X F,pp.1-89.)
[3] 宫希,蒋云峰,徐彬杰,等.2017.利用普通六倍体小麦和西藏半野生小麦杂交衍生的重组自交系定位小麦芒长QTL[J].作物学报,43(04):496-500.
(Gong X,Jiang Y F,Xu B J,et al.2017.The recombinant inbred lines derived from common hexaploid wheat and Tibetan semi-wild wheat were used to locate wheat awn length QTL[J].Crop Journal,43(04):496-500.)
[4] 金迪,王冬至,王焕雪,等.2019.小麦芒长抑制基因B2的精细定位与候选基因分析[J].作物学报 45(06):807-817.
(Jin D,Wang D Z,Wang H X,et al.2019.Fine mapping and candidate gene analysis of awn length suppressor gene B2 in wheat[J].Crop Journal,45(06):807-817.)
[5] 徐艳,徐继法,陈磊,等.2017.小麦TILLING突变体检测中基因组DNA提取方法的优化[J].麦类作物学报,37(02):185-191.
(Xu Y,Xu J F,Chen L,et al.2017.Optimization of genomic DNA extraction method in wheat TILLING mutant detection[J].Journal of Wheat Crops,37 (02):185-191.)
[6] 张礼.2016.高通量RNA-seq测序数据的基因表达水平分析研究[D].博士学位论文,南京航空航天大学,导师:陈松灿,pp.1-131.
(Zhang L.2016.Analysis of gene expression level in high-throughput RNA-seq sequencing data[D].Master's thesis,Nanjing University of Aeronautics and Astronautics,mentor:Chen Songcan,pp.1-131.)
[7] Ali M A,Hussain M,Khan M,et al.2010.Source-sink relationship between photosynthetic organs and grain yield attributes during grain filling stage in spring wheat (Triticum aestivum)[J].International Journal of Agriculture and Biology,12(4):509-515.
[8] Bessho-Uehara K,Wang D R,Furuta T,et al.2016.Loss of function at RAE2,a previously unidentified EPFL,is required for awnlessness in cultivated Asian rice[J].Proceedings of the National Academy of Sciences,113(32):8969-8974.
[9] Cao L,Hayashi K,Tokui M,et al.2016.Detection of QTLs for traits associated with pre-harvest sprouting resistance in bread wheat (Triticum aestivum L.)[J].Breeding Science,66(2):260-270.
[10] DeWitt N,Guedira M,Lauer E,et al.2019.Sequence-based mapping identifies a candidate transcription repressor underlying awn suppression at the B1 locus in wheat[J].New Phytologist,225(1):326-339.
[11] Dockter C,Gruszka D,Braumann I,et al.2014.Induced variations in brassinosteroid genes define barley height and sturdiness,and expand the green revolution genetic toolkit[J].Plant physiology,166(4):1912-1927.
[12] Driscoll C J,Jensen N F.2011.Chromosomes associated with waxlessness,awnedness and time of maturity of common wheat[J].Canadian Journal of Genetics and Cytology,6(3):324-333.
[13] Duran C,Appleby N,Edwards D,et al.2009.Molecular genetic markers:Discovery,applications,data storage and visualisation[J].Current Bioinformatics,4(1):16-27.
[14] Elbaum R,Zaltzman L,Burgert I,et al.2007.The role of wheat awns in the seed dispersal unit[J].Science,316(5826):884-886.
[15] Huang D,Zheng Q,Melchkart T,et al.2019.Dominant inhibition of awn development by a putative zinc-finger transcriptional repressor expressed at the B1 locus in wheat[J].New Phytologist,225(1):340-355.
[16] Jin J,Hua L,Zhu Z,et al.2016.GAD1 encodes a secreted peptide that regulates grain number,grain length,and awn development in rice domestication[J].Plant Cell,28(10):2453-2463.
[17] Kato K,Miura H,Akiyama M,et al.1998.RFLP mapping of the three major genes,Vrn1,Q and B1,on the long arm of chromosome 5A of wheat[J].Euphytica,101(1):91-95.
[18] Lei M,Li H,Zhang L,et al.2015.QTL IciMapping:integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations[J].The Crop Journal,3(3):269-283.
[19] Li H,Han Y,Guo X,et al.2015.Genetic effect of locus B2 inhibiting awning in double-ditelosomic 6B of Triticum durum DR147[J].Genetic Resources and Crop Evolution,62(3):407-418.
[20] Liu Q,Han R,Wu K,et al.2018.G-protein βγ subunits determine grain size through interaction with MADS-domain transcription factors in rice[J].Nature Communications, 9(852):1-12.
[21] Millet E,Rong J K,Qualset C O,et al.2013.Production of chromosome-arm substitution lines of wild emmer in common wheat[J].Euphytica,190(1):1-17.
[22] Muller K J,Romano N,Gerstner O,et al.1995.The barley hooded mutation caused by a duplication in a homeobox gene intron[J].Nature,374(6524):727-730.
[23] Nishijima R,Ikeda T M,Takumi S.2018.Genetic mapping reveals a dominant awn-inhibiting gene related to differentiation of the variety anathera in the wild diploid wheat aegilopstauschii[J].Genetica,146(1):75-84.
[24] Peleg Z,Saranga Y,Fahima T,et al.2010.Genetic control over silica deposition in wheat awns[J].Physiologia Plantarum,140(1):10-20.
[25] Pertea M,Kim D,Pertea G M,et al.2016.Transcript-level expression analysis of RNA-seq experiments with hisat,stringtieand ballgown[J].Nature Protocols,11(9):1650-1667.
[26] Rebetzke G,Bonnett D,Reynolds M.2016.Awns reduce grain number to increase grain size and harvestable yield in irrigated and rainfed spring wheat[J].Journal of Experimental Botany,67(9):2573-2586.
[27] Roig C,Pozzi C,Santi L,et al.2004.Genetics of barley hooded suppression[J].Genetics,167(1):439-448.
[28] Schrager‐Lavelle A,Klein H,Fisher A,et al.2017.Grass flowers:An untapped resource for floral evo-devo[J].Journal of Systematics and Evolution,55(6):525-541.
[29] Sourdille P,Cadalen T,Gay G,et al.2002.Molecular and physical mapping of genes affecting awning in wheat[J].Plant Breeding,121(4):320-324
.[30] Tanaka W,Toriba T,Ohmori Y,et al.2012.The YABBY gene TONGARI-BOUSHI1 is involved in lateral organ development and maintenance of meristem organization in the rice spikelet[J].Plant Cell,24(1):80-95.
[31] Toriba T,Hirano H Y.2014.The DROOPING LEAF and OsETTIN2 genes promote awn development in rice[J].The Plant Journal,77(4):616-626.
[32] Wang D,Yu K,Jin D,et al.2019.Natural variation in the promoter of awn length inhibitor 1 (ALI-1) is associated with awn elongation and grain length in common wheat[J].The Plant Journal,101(5):1075-1090.
[33] Wang T,Zou T,He Z,et al.2018.GRAIN LENGTH AND AWN 1 negatively regulates grain size in rice[J].Journal of Integrative Plant Biology,61(10):1036-1042.
[34] Watkins A E,Ellerton S.1940.Variation and genetics of the awn in Triticum[J].Journal of Genetics,40(1):243-270.
[35] Yoshioka M,Iehisa J C M,Ohno R,et al.2017.Three dominant awnless genes in common wheat:Fine mapping,interaction and contribution to diversity in awn shape and length[J].PLOS ONE,12(4):e0176148.