|
|
|
| Identification and Molecular Cytogenetic Analysis of Wheat-rye (Triticum aestivum-Secale cereale) 1RS-1BS/1BL Small Fragment Translocation Lines Against Fusarium Head Blight |
| LV Ting-Ting*, ZHANG Zhen-Yue*, YAO Xiao-Ni, ZHANG Han-Bing, LI Jia-Chuang, LIU Shu-Hui, YANG Qun-Hui, WU Jun, CHEN Xin-Hong** |
| Shaanxi Key Laboratory of Plant Genetic Engineering Breeding, College of Agronomy, Northwest A&F University, Yangling 712100, China |
|
|
|
|
Abstract Fusarium head blight (FHB) is one of the main diseases which caused a serious threat to the normal growth and yield formation of wheat (Triticum aestivum; 2n=6x=42, AABBDD). Rye (Secale cereal; 2n=2x=14, RR) with developed root system, strong stress resistance and disease resistance, is an important genetic resource for improving wheat traits. For the purpose of improving the FHB resistance in wheat, Mexican rye and common wheat W770B were hybridized to obtain several derived lines with genetic stability through multi-generation breeding. At the blooming stage of wheat, single flower inoculation (SFI) was used to identify the parents and progenies resistance to FHB. The results showed that the parents rye had moderate resistance to FHB whereas W770B showed high sensitivity, and the 12-5-1 derived line showed moderate resistance. Morphological and cytogenetic identification and analysis were conducted in 12-5-1 derived line by genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH) sequence amplification, sequence characterized amplified regions (SCAR), simple sequence repeats (SSR) molecular markers. The results showed that the root tip cell of 12-5-1 contained 42 chromosomes (2n=42). In the first meiotic metaphase of pollen mother cell, there is a proper chromosome pairing forming 21 bivalents(2n=42=21Ⅱ), and normal separation. GISH identification was performed on 12-5-1 using rye genome as a probe. Yellow and green signals were observed at the ends of two chromosomes of wheat, indicating that there is a translocation between wheat chromosome and rye chromosome. FISH results showed that the translocation of 1BS of wheat and 1RS of rye occurred in 12-5-1. Therefore, it is inferred that the hybridization of wheat and rye resulted in translocation of small chromosome segments. Moreover, Rye specific marker AF1/AF4 and SCAR marker of 1RS were used for identification and the corresponding bands were amplified in rye and 12-5-1 derived line. SSR analysis showed that three pairs of primers (Xgwm11、Xgwm31 and Xgwm550) on the 1BS chromosome of wheat could not be used to amplify the target bands in 12-5-1, while the remaining primers could be used to amplify the corresponding target bands, which indicated that 12-5-1 was a 1RS-1BS/1BL translocation line in Rye-Wheat. The creation of the derived line with FHB resistance provides a new germplasm resource for wheat breeding against FHB resistance.
|
|
Received: 01 July 2019
|
|
|
|
Corresponding Authors:
** cxh2089@126.com
|
| About author:: * The authors who contribute equally |
|
|
|
[1] 高立贞. 1980. 部分小麦近缘属植物及小偃麦杂种对小麦赤霉病的抗病性鉴定[J].陕西农业科学, (02):19-22.
(Gao L Z, 1980. Identification of disease resistance of some wheat relatives and small buckwheat hybrids to wheat scab [J],Shaanxi Agricultural Sciences, (02): 19-22.)
[2] 贾举庆, 2010. 小麦-非洲黑麦渐渗系的鉴定与抗条锈基因的分子作图[D].博士学位论文, 电子科技大学, 导师: 杨足君, pp. 17-23.
(Jia Y Q, 2010. Identification and molecular mapping of yellow rust new resistance genes in wheat-secale africanum introgression lines[D].Thesis for Ph.D., University of Electronic Science and Technology of China, Supervisor: Yang Z J, pp.17-23.)
[3] 雷孟平, 李光蓉, 刘成, 等, 2013. 抗条锈病的小麦-非洲黑麦异代换系的分子细胞学鉴定[J].农业生物技术学报, 21(3): 263-271.
(Lei M P, Li G R, Liu C,et al.2013. Molecular cytogenetic characterization of a Triticum durum-Secale africanum substitution line for resistance to sripe rust[J].Journal of Agricultural Biotechnology,21(3): 263-271.)
[4] 李韬, 李嫒嫒, 李磊, 2016.小麦赤霉病:从表型鉴定到抗性改良[J].科技导报, 34(22): 75-80.
(Li T, Li Y Y, Li L, 2016. Fusarium head blight in wheat: From phenotyping to resistance improvement[J].Science and Technology Review, 34(22): 75-80.)
[5] 刘登才, 郑有良, 王志容, 等. 2001. 影响小麦赤霉病抗性的Lophopyrum elongatum染色体定位[J].四川农业大学学报, (03): 200-205.
(Liu D C, Zheng Y L, Wang Z R, et al. 2001. Distribution of chromosomes in diploid Lophopyrum elongatum#[J].Journal of Sichuan Agricultural University, (03): 200-205.)
[6] 刘万才, 刘振东, 黄冲, 等. 2016. 近10年农作物主要病虫害发生危害情况的统计和分析[J].植物保护, 42(05): 1-9; 46.
(Liu W C, Liu Z D, Huang C, et al.2016. Statistics and analysis of crop yield losses caused by main diseases and insect pests in recent 10 years[J]. Plant Protection, 42(05): 1-9; 46.)
[7] 牛皓, 姜玉梅, 牛吉山. 2020. 小麦抗赤霉病遗传育种研究进展[J].农业生物技术学报, 28(03): 530-542.
(Niu H, Jiang Y M, Niu J S.2020. Research advances in the genetics and breeding of wheat (Triticum aestivum) resistance to Fusarium head blight[J]. Journal of Agricultural Biotechnology, 28(03): 530-542.)
[8] 武军, 王辉, 刘伟华, 等. 2006. 小麦新种质4844中外源P染色质的GISH与SSR分析[J].西北植物学报, 26(6):1093-1097.
(Wu J, Wang H, Liu W H, et al.2006. GISH and SSR analysis of exogenous P chromatin in the new wheat germplasm 4844[J].Northwest Plant Journal, 26(6): 1093-1097.)
[9] 徐健容, 叶华智. 1998. 小麦近缘种属对赤霉病菌的抗性评价[J].四川农业大学学报, (03): 36-41.
(Xu J R, Ye H Z. 1998. Evaluation of resistance of wheat relatives to Fusarium# [J]. Journal of Sichuan Agricultural University, (03): 36-41.)
[10] 闫红飞. 2009. 小麦抗叶锈基因Lr38、Lr45分子标记及抗病相关分析[D].博士学位论文.河北农业大学,导师:刘大群, pp. 8-13.
(Yan H F.2009. Molecular markers for Lr38,Lr45 and their resistance relate analysis against wheat leaf rust[D].Thesis for Ph.D., Hebei Agricultural University, Supervisor: Liu D Q, pp.8-13.)
[11] 张爱民, 阳文龙, 李欣, 等.2018. 小麦抗赤霉病研究现状与展望[J]. 遗传, 40(10): 858-873.
(Zhang I M,Yang W L, Li X, et al.2018. Current status and perspective on research against Fusarium head blight in wheat[J]. Hereditas, 40(10): 858-873.
[12] 张昊. 2011. 中国麦类赤霉病菌群体遗传多样性及生态适应性研究[D]. 博士学位论文, 中国农业科学院, 导师: 冯洁, pp. 50-55.
(Zhang H.2011. Population genetic diversity and ecological adapation of Fusarium graminearum species complex from wheat and barley in chain[D], Thesis for Ph.D., Chinese Academy of Agricultural Sciences, Supervisor: Feng J, pp. 50-55.)
[13] 郑殿升, 盛锦山. 2002. 主要作物远缘杂交概况[J].植物遗传资源科学, (01): 55-60.
(Zheng D S, Sheng J S. 2002. Overview of distant hybridization of main crops [J]. Plant Genetic Resources Science, (01): 55-60.)
[14] 周阳, 何中虎, 张改生, 等. 2004. 1BL/1RS易位系在我国小麦育种中的应用[J].作物学报, 30(6): 531-535.
(Zhou Y, He Z H, Zhang G S, et al.2004. Utilization of 1BL/1RS translocation in wheat breeding in China[J]. Acta Agronomica Sinica, 30(6): 531-535.)
[15] Ceoloni C, Forte P, Kuzmanović L, et al.2017. Cytogenetic mapping of a major locus for resistance to Fusarium head blight and crown rot of wheat on Thinopyrum elongatum 7EL and its pyramiding with valuable genes from a Thponticum homoeologous arm onto bread wheat 7DL[J]. Theoretical & Applied Genetics, 130(10): 2005-2024.
[16] Chai J, Zhou R, Jia J, Liu X.2006. Development and application of a new codominant PCR marker for detecting 1BL· 1RS wheat-rye chromosome translocations[J].Plant Breeding, 125(3): 302~304.
[17] Fu C M, Chen Z X, Liu D D, et al.2015. Clonal origin and evolution of myelodysplastic syndrome analyzed by dysplastic morphology and fluorescence in situ hybridization[J]. International Journal of Hematology,101(1): 58-66.
[18] Cota-Sánchez J H, Remarchuk K, Ubayasena K.2006. Ready-to-use DNA extracted with a CTAB method adapted for herbarium specimens and mucilaginous plant tissue[J].Plant Molecular Biology Reporte, 24: 161~167.
[19] David R F, BozorgMagham A E, Schmale III D G, et al.2016. Identification of meteorological predictors of Fusarium graminearum ascospore release using correlation and causality analyses[J]. European Journal of Plant Pathology, 145: 483-492.
[20] Dai Y, Duan Y, Liu H, et al.2017. Molecular cytogenetic characterization of two Triticum-Secale-Thinopyrum Trigeneric hybrids exhibiting superior resistance to Fusarium head blight, leaf rust, and stem rust race Ug99[J]. Frontiers in Plant Science, 8:7~97.
[21] Jia L J, Tang H Y, Wang W Q, et, al.2019. A linear nonribosomal octapeptide from Fusarium graminearum facilitates cell-to-cell invasion of wheat[J]. Nature Communications, 10(1): 922.
[22] Jiang Q T, Wei Y M, Andre L, et al.2010. Characterization of ω-secalin omega secalin genes from rye, triticale and a wheat 1BL/1RS translocation line[J].Journal of Applied Genetics, 51: 403~411.
[23] Kage U, Yogendra K N, Kushalappa A C.2017. TaWRKY 70 transcription factor in wheat QTL-2DL regulates downstream metabolite biosynthetic genes to resist Fusarium graminearum infection spread within spike[J]. Scientific Reports, 7: 42~596.
[24] Katto M C, Endo T R, Nasuda S.2004. A PCR-based marker for targeting small rye segments in wheat background[J].Genes & genetic systems, 79(4): 245~250.
[25] Lelley T, Eder C, Grausgruber H.2004. Influence of 1BL.1RS wheat-rye chromosome translocation on genotype by environment interaction[J]. Journal of Cereal Science, 39:313-320.
[26] Pestsova E, Ganal M W, Rder M S.2000. Isolation and mapping of microsatellite markers specific for the D genome of bread wheat[J]. Genome, 43: 689-697.
[27] Röder M S, Korzun V, Wendehake K, et al.1998. A microsatellite map of wheat[J]. Genetics, 149(4): 2007-2023.
[28] Tahmasebi S, Heidari B, Pakniyat H, et al.2015. Consequences of 1BL/1RS translocation on agronomic and physiological traits in wheat[J]. Cereal Research Communications, 43(4): 554-566.
[29] Tang Z X, Yang Z J, Fu S L.2014. Oligonucleotides replacing the roles of repetitive sequences pAs1,pSc119.2,pTa-535,pTa71,CCS1,and pAWRC.1 for FISH analysis[J].Journal of Applied Genetics, 55(3): 313-318.
[30] Terasawa Y, Ito M, Tabiki T, et al.2016. Mapping of a major QTL associated with protein content on chromosome 2B in hard red winter wheat (Triticum aestivum L.)[J].Breeding Science, 66(4): 471-480.
[31] Wang H, Sun S, Ge W, et al.2020. Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat[J]. Science, 368: eaba5435. |
|
|
|
