Polymorphism of 3'-UTR Region of BMPR-IB Gene and Its Correlation with Litter Size in Four Sheep (Ovis aries) Varieties
SUN Wei-Bo1, ZHANG Li-Ping1*, LANG Xia2, ZHANG Xiao-Yan1, JIN Ji-Peng1, ZHANG Zi-Qi1, XIAO Fan1, LEI Zhi-Hui1
1 College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2 Institute of Animal and Pasture Science and Green Agriculture, Gansu Academy of Agricultural Science, Lanzhou 730070, China
Abstract In order to explore the genetic effects of the untranslated region polymorphism of bone morphogenetic protein receptor IB (BMPR-IB) gene on the reproductive performance of sheep (Ovis aries), Multiple Hu Sheep (HM), Singleton Mongolian Sheep (MS), Twins Mongolian Sheep (MT), Singletons Oula Sheep (ZS), Twins Oula Sheep (ZT) and Singletons Australian Sheep (AS) was selected in this study to detect SNPs in the 3'-UTR of BMPR-IB gene by DNA sequencing, the correlation between polymorphism loci and litter size was also studied. Two mutation sites of 1339 (G/A) and 1354 (A/G) were detected at 1 145~1 500 bp in the 3'-UTR region of BMPR-IB gene, and further analysis was performed on the 1354 locus (corresponding to the 3019 locus in the mRNA sequence of BMPR-IB gene NM_001009431.1). The results showed that wild genotype AA, heterozygous mutant genotype AG and homozygous mutant genotype GG were detected in MT and ZT, the dominant allele was A; wild genotype AA and heterozygous mutation genotype AG were detected in MS, ZS and AS, the dominant allele was A; heterozygous mutation genotype AG and homozygous mutation genotype GG were detected in HM, the dominant allele was G. The result of polymorphism information content (PIC) analysis showed that HM, MT, MS, ZS and AS were in low polymorphism (PIC<0.25); and ZT was in moderate polymorphism (0.25<PIC<0.5). There was a significant difference in the 1354 locus of 3'-UTR region of BMPR-IB gene among the multiple-lamb, twins-lamb and singletons-lamb populations (P<0.05). The genotype of AG and GG had significant effects on the fecundity in HM, MT and ZT. These results suggested that the 1354 locus in the 3'-UTR region of BMPR-IB gene might be regarded as a molecular genetic marker locus for multiple-lamb and twins-lamb in sheep. This study provides a theoretical scientific basis for breeding sheep with high fecundity.
SUN Wei-Bo,ZHANG Li-Ping,LANG Xia, et al. Polymorphism of 3'-UTR Region of BMPR-IB Gene and Its Correlation with Litter Size in Four Sheep (Ovis aries) Varieties[J]. 农业生物技术学报, 2020, 28(4): 702-710.
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