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| Development and Application of Duplex Real-time PCR Assay for the Detection of Genetically Modified Soybean (Glycine max) MON87701 and MON87708 |
| YUAN Jun-Jie1,2, WEI Shuang3, LONG Yang1, WU Xi-Yang4, LI Xiang5, FU Wei2* |
1 Zhanjiang Customs, Zhanjiang 524022, China;
2 Chinese Academy of Inspection and Quarantine, Beijing 100029, China;
3 Guangzhou Customs, Guangzhou 510000, China;
4 College of Science & Engineering, Jinan University, Guangzhou 510000, China;;
5 Shanghai Customs, Shanghai 200000, China |
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Abstract China is one of the main consumer of genetically modified soybean (Glycine max) and the safety of genetically modified soybeans received wider attention. It is imperative to study fast and accurate detection methods for genetically modified ingredients in soybeans. In this study, based on TaqMan probe technology, a duplex real-time PCR method was established to detect MON87701 and MON87708 transgenic soybean strains simultaneously, and its specificity, sensitivity and applicability were analyzed. The results showed that the method could accurately detect target genes from 18 transgenic samples and 5 non-transgenic samples, indicating good specificity. The sensitivity test showed that the sensitivity of this method was 0.01%. The applicability of this method had been tested by 10 real samples and the results were as same as the EU standard. These results suggested that the duplex real-time PCR method was an effective tool for detection of soybean MON87701 and soybean MON8770 in the exit and entry inspection laboratory. This study provides a reference for the regulation of genetically modified products.
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Received: 13 June 2019
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Corresponding Authors:
*fuwei0212@163.com
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