|
|
Advances in the Differentiation and Regulation of Porcine (Sus scrofa) Adipocytes |
GONG Jian-Fei1,2, LIU Xin1*, SUN Jin-Hai2, WANG Li-Xian1* |
1 Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2 College of Animal Science, Qingdao Agricultural University, Qingdao 266109, China |
|
|
Abstract Fat tissue accumulation is closely related to growth efficiency and meat quality in pigs (Sus scrofa). The development of adipose tissue depends on the increasing of adipocytic numbers and size, which leads to the adipogenesis through coordination and interaction. Adipocyte differentiation is controlled by a precisely regulated transcriptional cascade that activates or inhibits each other's expression in a continuous manner. The process of porcine adipocyte differentiation, the research model of adipocytes, the method of preadipocytes differentiation, the marker factors of adipocyte differentiation, and the research progress of several important transcription factors related to porcine adipocyte differentiation were reviewed in this paper, which aims to provide the reference for studying the mechanism of differentiation of pig adipocytes and lipid metabolism.
|
Received: 19 July 2019
|
|
Corresponding Authors:
*firstliuxin@163.com; iaswlx@263.net
|
|
|
|
[1] 陈妍, 张国华, 陆会宁, 等. 2014. 八眉猪肌内前体脂肪细胞的分离培养及诱导分化[J]. 广东农业科学, 41(13): 110-113. (Chen Y, Zhang G H, Lu H N, et al.2014. Culture and induced differentiation of intramuscular preadipocytes from Bamei pigs in vitro[J]. Guangdong Agricultural Sciences, 41(13): 110-113.) [2] 姜俊芳. 2006. 脂肪细胞分化相关因子基因表达在猪生长过程中的变化规律研究[D]. 博士学位论文, 浙江大学, 导师: 许梓荣, pp. 47-49. (Jiang J F.2006. Study on gene expression rules of adipocyte determination factors in pig development[D]. Thesis for Ph.D., Zhejiang University, Supervisor: Xu Z R, pp. 47-49.) [3] 李昌辉. 2012. 猪脂肪细胞体外培养及葡萄糖和胰岛素诱导生脂研究[D]. 博士学位论文, 西北民族大学, 导师: 卢建雄, pp.21-30. (Li C H.2012. Study on culture of porcine adipocytes in vitro and lipogenesis induced by glucose and insulin[D]. Thesis for Ph.D., Northwest Minzu University, Supervisor: Lu J X, pp. 21-30.) [4] 刘新农, 刘秀, 李天佳, 等. 2016. 诱导剂作用时间对3T3-L1前脂肪细胞系分化的影响[J]. 中国医科科学院学报, 38(3): 271-274. (Liu X N, Liu X, Li T J, et al.2016. Impact of the action time of inducers on differentiation of 3T3-L1 cells[J]. Acta Academiae Medicinae Sinicae, 38(3): 271-274.) [5] 卢慧玲, 王宏伟, Cianflone K, 等. 2004. 促酞化蛋白(ASP)诱导3T3-L1前脂肪细胞分化[J]. 中国生物化学与分子生物学报, 20(3): 383-386. (Lu H L, Wang H W, Cianflone K, et al.2004. Acylation stimulating protein inducing 3T3-L1 preadipocyte differentiation[J]. Chinese Journal of Biochemistry and Molecular Biology, 20(3): 383-386.) [6] 卢慧玲, 王宏伟, Cianflone K, 等. 2006. 诱导 SW872前脂肪细胞分化的最优方法探讨[J]. 基础医学与临床, 26(3): 315-318. (Lu H L, Wang H W, Cianflone K, et al.2006. To optimize the method for inducing SW872 preadipocyte to differentiation[J]. Basic & Clinical Medicine, 26(3): 315-318.) [7] 盘道兴, 王振, 杨茂林, 等. 2017. 不同品种猪 PPARγ和C/EBPα基因表达规律与肌内脂肪含量的相关[J]. 中国农业科学, 50(1): 171-182. (Pan D X, Wang Z, Yang M L, et al.2017. Association of the PPARγ and C/EBPα gene expression with intramuscular fat content in different varieties of pig[J]. Scientia Agricultura Sinica, 50(1): 171-182.) [8] 屈长青, 张国华, 陈粉粉, 等. 2005. 猪前体脂肪细胞的原代培养[J]. 农业生物技术学报, 13(5): 649-653. (Qu C Q, Zhang G H, Chen F F, et al.2005. Primary culture of porcine preadipocyte[J]. Journal of Agricultural Biotechnology, 13(5): 649-653.) [9] 孙超. 2001. ECM组分和cAMP对大鼠前体脂肪细胞增殖与分化的调控[D]. 博士学位论文, 西北农林科技大学, 导师: 杨公社. pp. 36-44. (Sun C.2001. Regulation of mice preadipocytes' proliferation and differentiation by ECM components and cAMP[D]. Thesis for Ph.D., Northwest Sci-Tech University of Agriculture and Forestry, Supervisor: Yang G S, pp. 36-44.) [10] 王竹晨, 刘建中, 李燕, 等. 2001. 人前脂肪细胞的原代培养[J]. 中山大学学报(医学科学版), 22(6): 443-446. (Wang Z C, Liu J Z, Li Y, et al.2001. Primary culture of human preadipocyte[J]. Journal of Sun Yat-sen University (Medical Science), 22(6): 443-446.) [11] 夏成, 王哲, 朱淑玲, 等. 2004. 犊牛前脂肪细胞的培养及其增殖与分化模型的建立[J]. 中国兽医科学, 34(5): 26-30. (Xia C, Wang Z, Zhu S L, et al.2004. Culture of calf preadipocyte and establishment of its proliferation and differentiation model[J]. Chinese Journal of Veterinary Science and Technology, 34(5): 26-30.) [12] 张乐, 彭燮, 刘扬, 等. 2014. 牛A-FABP与SREBP1基因的组织表达规律研究[J]. 家畜生态学报, 35(11): 18-23. (Zhang L, Peng X, Liu Y, et al.Gene expression patterns of A-FABP and SREBP1 in cattle tissues[J]. Acta Ecologiae Animalis Domastici, 35(11): 18-23.) [13] 张宁芳, 成志敏, 乐宝玉, 等. 2018. 猪肌源性前体脂肪细胞的分离培养和成脂诱导分化研究[J]. 畜牧兽医学报, 49(12): 83-92. (Zhang N F, Cheng Z M, Le B Y, et al.2018. Isolation, culture and adipogenic differentiation of pig myogenic preadipocytes cell[J]. Acta Veterinaria Et Zootechnica Sinica, 49(12): 83-92.) [14] 赵蕾, 郑美丽, 杨梅, 等. 2019. 3T3-L1前体脂肪细胞诱导分化方法优化的初步探讨[J]. 首都医科大学学报, 40(02): 74-79. (Zhao L, Zheng M L, Yang M, et al.2019. Optimization of induction and differentiation of 3T3-L1 preadipocyte[J]. Journal of Capital Medical University, 40(02):74-79. [15] Aggarwal S, Pittenger M F.2005. Human mesenchymal stem cells modulate allogeneic immune cell responses[J]. Blood, 105(4): 1815-1822. [16] Anderson D B, Kanffman R G.1973. Cellular and enzymatic changes in porcine adipose tissue during growth[J]. Journal of Lipid Research, 14(2): 160-168. [17] Bauerle K T, Hutson I, Scheller E L, et al.2018. Glucocorticoid receptor signaling is not required for in vivo adipogenesis[J]. Endocrinology, 159(5): 2050-2061. [18] Boone C, Mourot J, Gregiore F, et al.2000. The adipose conversion process: Regulation by extracellular and intracellular factors[J]. Reproduction Nutrition Development, 40(4): 325-358. [19] Chakravarty K, Leahy P, Becard D, et al.2001. Sterol regulatory element binding proteins-1c mimics the negative effect of insulin on phosphoenolpyruvate carboxykinase (GTP) gene transcription[J]. Journal of Biological Chemistry, 276(37): 34816-34823. [20] Chen X L, Luo Y L, Jia G, et al.2017. The effect of arginine on the Wnt/β-catenin signaling pathway during porcine intramuscular preadipocyte differentiation[J]. Food & Function, 8(1): 381-386. [21] Chu W W, Wei W, Han H Y, et al.2017. Muscle-specific downregulation of GR levels inhibits adipogenesis in porcine intramuscular adipocyte tissue[J]. Science Report, 7(1): 510. [22] Corrale P, Vidalpuig A, Medinagómez G.2018. PPARs and metabolic disorders associated with challenged adipose tissue plasticity[J]. International Journal of Molecular Sciences, 19(7): E2124. [23] Cui J, Chen W, Liu J, et al.2016. Study on quantitative expression of PPARγ and ADRP in muscle and its association with intramuscular fat deposition of pig[J]. SpringerPlus, 5(1): 1501. [24] Di S B, Sardina J L, Van O C, et al.2014. C/EBPα poises B cells for rapid reprogramming into induced pluripotent stem cells[J]. Nature, 506(7487): 235-239. [25] Dong P Y, Mai Y, Zhang Z Y, et al.2014. MiR-15a/b promote adipogenesis in porcine pre-adipocyte via repressing FoxO1[J]. Acta Biochimica et Biophysica Sinica, 46(7): 565-571. [26] Francisco V, Figueirinha A, Costa G, et al.2016. The flavone luteolin inhibits liver X receptor activation[J]. Journal of Natural Products, 79(5): 1423-1428. [27] Green H, Meuth M.1974. An established pre-adipose cell line and its differentiation in culture[J]. Cell, 3(2): 127-33. [28] Hadrich F, Sayadi S.2018. Apigetrin inhibits adipogenesis in 3T3-L1 cells by downregulating PPARγ and CEBP-α[J]. Lipids in Health and Disease, 17(1): 95. [29] Hamm J K, Park B H, Farmer S R.2001. A role for C/EBPβ in regulating peroxisome proliferator-activated receptor γ activity during adipogenesis in 3T3-L1 preadipocytes[J]. Journal of Biological Chemistry, 276(21): 18464-18471. [30] Han H Y, Gu S S, Chu W W, et al.2017. miR?17?5p regulates differential expression of NCOA3 in pig intramuscular and subcutaneous adipose tissue[J]. Lipids, 52(11): 1-11. [31] Hao Q, Hansen J B, Petersen R K, et al.2010. ADD1/SREBP1c activates the PGC1-alpha promoter in brown adipocytes[J]. Biochimica et Biophysica Acta, 1801(4): 421-429. [32] Hausman G J, Campion D R, Mcnamara J P, et al.1981. Adipose tissue development in the fetal pig after decapition[J]. Journal of Animal Science, 53(6): 1634-1644. [33] Hausman G J, Dodson M V, Ajuwon K, et al.2009. The biology and regulation of preadipocytes and adipocytes in meat animals[J]. Journal of Animal Science, 87(4): 1218-46. [34] Hausman G J, NovakofskI J E, Martin R J, et al.1984. The development of adipocytes in primary stromal-vascular culture of fetal pig adipose tissue[J]. Cell and Tissue Research, 236(2): 459-464. [35] He K, Wang Q S, Wang Z, et al.2013. Association study between gene polymorphisms in PPAR signaling pathway and porcine meat quality traits[J]. Mammalian Genome, 24(7-8): 322-331. [36] Hitoshi S.2001. Sterol regulatory element-binding proteins (SREBPs): Transcriptional regulators of lipid synthetic genes[J]. Progress in Lipid Research, 40(6): 439-452. [37] Houseknecht K L, Bidwell C A, Portocarrero C P, et al.1998. Expression and cDNA cloning of porcine peroxisome proliferator-activated receptor gamma (PPARgamma)[J]. Gene, 225(1-2): 89-96. [38] Hudak C S, Gulyaeva O, Wang Y H, et al.2014. Pref-1 marks very early mesenchymal precursors required for adipose tissue development and expansion[J]. Cell Report, 8(3): 678-687. [39] Hung S C, Chang C F, Ma H L, et al.2004. Gene expression profiles of early adipogenesis in human mesenchymal stem cells[J]. Gene, 340(1): 141-150. [40] Jiang S Z, Wei H K, Song T X, et al.2015. KLF13 promotes porcine adipocyte differentiation through PPARγ activation[J]. Cell & Bioscience, 5(1): 28. [41] Kociucka B, Jackowiak H, Kamyczek M, et al.2016. The relationship between adipocyte size and the transcript levels of SNAP23, BSCL2, and COPA genes in pigs[J]. Meat Science, 121: 12-18. [42] Kouba M, Bonneau M.2009. Compared development of intermuscular and subcutaneous fat in carcass and primal cuts of growing pigs from 30 to 140 kg body weight[J]. Meat Science, 81(1): 270-274. [43] Kouba M, Sellier P.2011. A review of the factors influencing the development of intermuscular adipose tissue in the growing pig[J]. Meat Science, 88(2): 213-220. [44] Lee J E, Ge K.2014. Transcriptional and epigenetic regulation of PPARγ expression during adipogenesis[J]. Cell & Bioscience, 4(1): 29. [45] Madsen M S, Siersbæk R, Boergesen M, et al.2014. Peroxisome proliferator-activated receptor γ and C/EBPα synergistically activate key metabolic adipocyte genes by assisted loading[J]. Molecular & Cellular Biology, 34(6): 939-954. [46] Mano H, Kimura C, Fujisawa Y, et al.2000. Cloning and function of rabbit peroxisome proliferator-activated receptor δ/β in mature osteoclasts[J]. Journal of Biological Chemistry, 275(11): 8126-8132. [47] Nakamura T, Shiojima S, Hirai Y, et al.2003. Temporal gene expression changes during adipogenesis in human mesenchymal stem cells[J]. Biochemical Biophysical Research Communications, 303(1): 306-312. [48] Pan S F, Cui Y X, Dong X, et al.2017. MicroRNA-130b attenuates dexamethasone-induced increase of lipid accumulation in porcine preadipocytes by suppressing PPAR-γ expression[J]. Oncotarget, 8(50): 87928-87943. [49] Rosen E D, Macdougald O A.2006. Adipocyte differentiation from the inside out[J]. Nature Reviews Molecular Cell Biology, 7(12): 885-896. [50] Rosen E D, Spiegelman B M.2001. PPARγ: A nuclear regulator of metabolism, differentiation, and cell growth[J]. Journal of Biological Chemistry, 276(41): 37731-37734. [51] Rosen E D, Walkey C J, Puigserver P, et al.2000. Transcriptional regulation of adipogenesis[J]. Genes & Development, 14(11): 1293-1307. [52] Ross S E, Hemati N, Longo K A, et al.2000. Inhibition of adipogenesis by Wnt signaling[J]. Science, 289(5481): 950-953. [53] Sarjeant K, Stephens J M.2012. Adipogenesis[J]. Cold spring harbor perspectives in biology, 4(9): a008417. [54] Siersbæk R, Nielsen R, Mandrup S.2012. Transcriptional networks and chromatin remodeling controlling adipogenesis[J]. Trends in Endocrinology Metabolism, 23(2): 56-64. [55] Sun Y M, Qin J, Liu S G, et al.2017. PDGFRα regulated by miR-34a and FoxO1 promotes adipogenesis in porcine intramuscular preadipocytes through Erk signaling pathway[J]. International Journal of Molecular Sciences, 18(11): E2424. [56] Tang Q Q, Otto T C, Lane M D.2004. Commitment of C3H10T1/2 pluripotent stem cells to the adipocyte lineage[J]. Proceedings of the National Academy of Sciences of the USA, 101(26): 9607-9611. [57] Taylor S M, Jones P A.1979. Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine[J]. Cell, 17(4): 771-779. [58] Tong Q, Dalgin G, Xu H, et al.2000. Function of GATA transcription factors in preadipocyte-adipocyte transition[J]. Science, 290(5489): 134-138. [59] Tontonoz P, Hu E, Spiegelman B M.1994. Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor[J]. Cell, 79(7): 1147-1156. [60] Vishwanath D, Srinivasan H, Patil M S, et al.2013. Novel method to differentiate 3T3 L1 cells in vitro to produce highly sensitive adipocytes for a GLUT4 mediated glucose uptake using fluorescent glucose analog[J]. Journal of Cell Communication and Signaling, 7(2): 129-140. [61] Wei S J, Zheng Y Y, Zhang M M, et al.2018. Grape seed procyanidin extract inhibits adipogenesis and stimulates lipolysis of porcine adipocytes in vitro[J]. Journal of Animal Science, 96(7): 2753-2762. [62] Xiong Y, Pang W J, Wei N, et al.2013. Knockdown of both FoxO1 and C/EBPβ promotes adipogenesis in porcine preadipocytes through feedback regulation[J]. Cell Biology International, 37(9): 905-916. [63] Xu H F, Luo J, Zhao W S, et al.2016. Overexpression of SREBP1 (sterol regulatory element binding protein 1) promotes de novo fatty acid synthesis and triacylglycerol accumulation in goat mammary epithelial cells[J]. Journal of Dairy Science, 99(1): 783-795. [64] Yu L, Zhang L, Chu Y, et al.2017. Comparative analyses of long non-coding RNA in lean and obese pig[J]. Oncotarget, 8(25): 41440-41450. [65] Yue T, Fang Q, Yin J D, et al.2010. S-adenosylmethionine stimulates fatty acid metabolism-linked gene expression in porcine muscle satellite cells[J]. Molecular Biology Reports, 37(7): 3143-3149. [66] Zhang G H, Lu J X, Chen Y, et al.2014. Comparison of the adipogenesis in intramuscular and subcutaneous adipocytes from Bamei and Landrace pigs[J]. International Journal of Biochemistry and Cell Biology, 92(4): 259-267. [67] Zhang G H, Lu J X, Chen Y, et al.2016. Glucose and insulin stimulate lipogenesis in porcine adipocytes: Dissimilar and identical regulation pathway for key transcription factors[J]. Molecular Cells, 39(11): 797-806. [68] Zhang Y F, Yuan Z Q, Song D G, et al.2013. Effects of cannabinoid receptor 1 (brain) on lipid accumulation by transcriptional control of CPT1A and CPT1B[J]. Animal Genetics, 45(1): 38-47. [69] Zhu Y L, Chen T, Xiong J L, et al.2018. miR-146b inhibits glucose consumption by targeting IRS1 gene in porcine primary adipocytes[J]. International Journal of Molecular Sciences, 19(3):E783. [70] Zhu Y, Kan L, Qi C, et al.2000. Isolation and characterization of peroxisome proliferator-activated (PPAR) interacting protein (PRIP) as a coactivator for PPAR[J]. Journal of Biological Chemistry, 275(18): 13510-13516. |
|
|
|