猪<em>PRKAA1</em>基因相关LncRNAs的筛选及其在组织中的表达分析

doi:10.3969/j.issn.1674-7968.2020.02.005

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Abstract PRKAA1 (protein kinase, AMP activated α1) plays a very important regulatory role for the processes of fat metabolism, cell proliferation, fat formation, etc. In this study, Guizhou fatty pig breed Congjiang Xiang pig (Sus scrofa) and commercial pig breed Yorkshire pigs (S. scrofa) were taken as research objects. Based on the 3'UTR sequence of PRKAA1 (GenBank No.XM_021076521), NCBI and NONCODE (http://www.noncode.org/) were used to screen the LncRNAs related to PRKAA1. The screened LncRNAs were cloned, and the secondary structure, ORF, target site of PRKAA1 and regulated miRNAs were predicted. qRT-PCR was used to detect the screened LncRNAs expression in different tissues of Congjiang Xiang pigs and Yorkshire pigs. Two candidate LncRNAs, NONSUST030689.1 (named ALNC1) and NONSUST004358.1 (named ALNC2), were selected for next experiments. The sequenced results revealed that ALNC2 had 46 bp inserted compared with original sequence. ALNC1 had 4 ORFs, and ALNC2 had 5 ORFs. In addition, ALNC1 and ALNC2 each had 1 target site for PRKAA1. Predicted miRNAs which could bind with LncRNAs including miR-1226-5p, miR-487b-5p, miR-7156-5p, etc. The results of qRT-PCR showed that the 2 LncRNAs had universal expression in all tissues of Congjiang Xiang pig and Yorkshire pig and were significantly different among different tissues. In Congjiang Xiang pig, ALNC1 and ALNC2 were the highest expressed in the liver and spleen and the lowest in the longissimus dorsi muscle. But ALNC1 and ALNC2 were the highest expressed in the large intestine and lung and the lowest in the longissimus dorsi muscle in Yorkshire pig. ALNC1 had significant higher expression in liver, spleen, small intestine, fat, and longissimus dorsi muscle of Congjiang Xiang pigs than that of Yorkshire pigs (P<0.01), but lower expression in heart and large intestine than that of Yorkshire pigs (P<0.01), and no significant difference in kidney and lung. ALNC2 had significant higher expression in liver, kidney and longissimus dorsi muscle of Congjiang Xiang pigs than that of Yorkshire pigs (P<0.01), but lower expression in heart, lung, small intestine, and large intestine (P<0.01). In fat tissues, ALNC2 expression levels of Congjiang Xiang pig were significantly lower than that of Yorkshire pigs (P<0.05). The ALNC2 expression levels in spleen had no significant difference between Congjiang Xiang pigs and Yorkshire pigs. The present results could provide basic data for further studies on the regulatory role of PRKAA1 and LncRNAs in fat deposition process of pigs.

Key words： PRKAA1(protein kinase,AMP activated α1) LncRNA Differential expression Meat quality trait Fat deposition

Received: 02 July 2019

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S828.8+9

Corresponding Authors: *gzdxxhq@163.com

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	Articles by authors
	WU Xiao-Min
	NI Kai
	ZHU Xiao-Feng
	CHEN Ying-Lian
	ZHAO Jia-Fu
	XU Hou-Qiang

Cite this article:

WU Xiao-Min,NI Kai,ZHU Xiao-Feng, et al. Screening of Pig (Sus scrofa) PRKAA1 Gene-related LncRNAs and Their Expression Analysis in Tissues[J]. 农业生物技术学报, 2020, 28(2): 232-241.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2020.02.005 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2020/V28/I2/232

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