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| Association Analysis Between Polymorphisms of Follicle Stimulating Hormone Receptor Gene and Egg Production in White King Pigeon (Columba livia) |
| MAO Yue-Jiao1,2, LI Guo-Qin2,3, CHEN Xiao-Yan4, LI Gao-Lou5, WEN Ji-Hui4, TAO Zheng-Rong2, LI Hai-Ying1,*, LU Li-Zhi2,3,* |
1 College of Animal Science, Xingjiang Agricultural University, Urumqi 830052, China;
2 Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310000, China;
3 Key Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture of China, Hangzhou 301121, China;
4 Pingyang County Aofeng Pigeon Industry Co., Ltd., Wenzhou 325400, China;
5 Pingyang County Pigeon Association, Wenzhou 325400, China |
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Abstract The follicle stimulating hormone receptor (FSHR) is used to mediate the physiological functions of biomacromolecule follicle stimulating hormone, and has an important influence on the reproductive traits of animals. In order to analyze the relationship between single nucleotide polymorphisms (SNPs) of FSHR gene and egg production in White King Pigeon (Columba livia), this study selected 209 pigeons as the research subjects and used the direct sequencing of PCR products to screen the SNPs of exon 6, exon 9, exon 10 and their adjacent upstream and downstream sequence in FSHR gene. The results showed that 18 mutation sites were found in the 3 fragments detected and the amino acid sequences were changed accordingly. Among them, 3 mutation sites G67729A, A67801G, A67898G were found in exon 6 and its upstream and downstream sequences. 5 mutation sites C76462T, T76468C, G76527T, C76616T, T76651G were found in exon 9 and its upstream and downstream sequences. 10 mutation sites were found in exon 10, which were A82714G, C82930T, A83083C, G83098T, G83158A, C83200T, A83246C, T83395C, T83740C, C83766T. T76468C and C83766T had 2 genotypes AA, AB, and the rest had 3 genotypes: AA, AB, BB. A Hardy-Weinberg equilibrium test was performed on 18 loci and found to be in equilibrium except for the C76616T locus. Correlation analysis between polymorphic loci and average egg production showed that the homozygous BB genotype of A67801G locus was extremely significantly correlated with egg production (P<0.01), and the homozygous BB genotype of C76616T locus was significantly correlated with egg production (P<0.05). Therefore, the A67801G and C76616T loci in the FSHR gene could be utilized as candidate molecular marker sites for the selection and breeding of the egg-laying traits of White King Pigeons, which would provide a theoretical basis for marker-assisted breeding from the molecular level to better improve the breeding performance of the pigeon population and shorten the breeding years.
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Received: 13 December 2018
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Corresponding Authors:
* , lulizhibox@163.com; lhy-3@163.com
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