Isolation, Identification and Denitrification Performance of a Heterophic Nitrifying Bacillus megaterium
LI Zhong-Hui1,2, WANG Miao1, YI Meng-Meng1, WANG Rui-Ning1,2, CHEN Wen-Wei1,2, LU Mai-Xin1,*
1 Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture/Pearl River Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Guangzhou 510380, China; 2 College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China
Abstract Biology method is one of the most economic and effective ways for the treatment of aquaculture waste at present. Nitrifying bacteria plays an important role in the process of biological nitrogen removal. A highly efficient heterotrophic nitrifying bacterium P5-2 was isolated from the water of tilapia (Oreochromis niloticus) recirculation aquaculture systems through enrichment culture and plate purification. The strain was identified as Bacillus megaterium by morphological observation, physiological and biochemical reaction identification and 16S rRNA gene sequence analysis. The results of Gram staining showed that the strain was Gram-positive. The result of determination of extracellular enzyme showed that starch and casein could be utilized by the strain. When temperature was 30 °C, salinity was 10‰, and pH was 7, the strain grew fastest, and first reached the logarithmic growth period and the stable period. The results of nitrification experiments showed that environmental factors such as carbon source, carbon-nitrogen ratio and dissolved oxygen concentration could influence its rate of ammonia nitrogen removal. When sodium citrate was used as carbon source, carbon-nitrogen ratio was 15, pH was 7, and rotational speed was 180 r/min, the highest ammonia nitrogen removal rate of 99.07% was achieved after 24 h. A highly efficient heterotrophic nitrifying bacterium P5-2 was isolated in this study. Its nitrification performance was explored, which provides critical support for its application in aquaculture waste water treatment.
LI Zhong-Hui,WANG Miao,YI Meng-Meng, et al. Isolation, Identification and Denitrification Performance of a Heterophic Nitrifying Bacillus megaterium[J]. 农业生物技术学报, 2019, 27(8): 1331-1340.
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