基因编辑技术发展及其在家畜上的应用

doi:10.3969/j.issn.1674-7968.2019.01.015

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Abstract Several novel gene editing techniques have been applied in scientific research and livestock breeding in recent years, including zinc finger protein nuclease (ZFN), transcriptional activator-like effector nuclease (TALEN), clustered regularly interspaced short palindromic repeats/CRISPR associated proteins (CRISPR/Cas) system. These gene editing techniques site-directly edit genomes by recognizing the target sites according to the specific structure followed by endonuclease dissection,which is characterized by high efficiency, accuracy, and simple production. In this paper, we demonstrated the basic principles, differences and potential main problems of above three gene editing techniques, summarized the application in the main livestock pigs, cattle and sheep, and provided an outlook of the future of these techniques in application.

Key words： Gene editing ZFN TALEN CRISPR/Cas Livestock

Received: 31 July 2018

ZTFLH:

S854.5

Corresponding Authors: *, zhangyong1956@nwsuaf.edu.cn; quanfusheng@nwsuaf.edu.cn

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	XIE Xiao-Gang
	XUE Jia
	KANG Jian
	GE Lu-Xing
	DONG Xiang-Chen
	WANG Yu-Fei
	ZHANG Yong
	QUAN Fu-Sheng

Cite this article:

XIE Xiao-Gang,XUE Jia,KANG Jian, et al. Development of Gene Editing Techniques and Its Application in Livestock[J]. 农业生物技术学报, 2019, 27(1): 139-149.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2019.01.015 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2019/V27/I1/139

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