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| The Analysis of Splice Variants and Genomic Imprinting Status of NTM Gene in Cattle (Bos taurus) |
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Abstract Neurotrimin (NTM) plays an important role in the nerve development. The NTM is an imprinted gene in human (Homo sapiens), but the imprinting state in cattle (Bos taurus) has not been revealed. The purpose of present study is to analyze the sequences of cattle NTM gene, the structure of its coding protein, and to identify the imprinting status of NTM gene in adult tissues and placenta in cattle. A new splice NTMxd (GenBank No.: MH037574) of cattle NTM gene was obtained by the technology of rapid amplification of cDNA ends (RACE), and a more complete transmembrane signal was found in the N-terminal of amino acid sequence encoded by the splice. In order to analyze the imprinting status of NTM gene in cattle, a locus of single nucleotide polymorphism (SNP) was found in cattle NTM gene by direct sequencing of PCR products, the accession number of the SNP locus in NCBI website (www.ncbi.nlm.nih.gov) is rs42185569. The total RNA was extracted from the tissue samples and placentas of heterozygous individuals, and performed expression analysis by reverse transcription-PCR (RT-PCR). Direct sequencing of the RT-PCR amplified products revealed that NTM gene exhibited monoallelic expression in cattle placenta and adult tissues, indicating that NTM gene is an imprinted gene in cattle. The results of present study provides basic data for further studying of NTM gene function and related neurodevelopmental diseases.
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Received: 23 March 2018
Published: 26 September 2018
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