小麦粒重相关基因TaCYP78A16的克隆和CAPS标记开发

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Abstract Grain weight is an important factor determing the yield of wheat (Triticum aestivum) production, and is one of the main objectives in high-yield wheat breeding. In order to develop molecular markers of potential grain weight related genes in wheat, the TaCYP78A16 (Triticum aestivum cytochrome P450 78A 16, GenBank No.: MH572527) gene was isolated from the whole wheat genome, then polymorphism analysis was implemented in its genome sequence. The results showed that high expression of TaCYP78A16 was detected in young panicles immature seeds by real time PCR analysis expression pattern of TaCYP78A16 and it indicated that TaCYP78A16 might play an important role in grain development and have an impact on grain weight. Polymorphism analysis showed that there were 7 polymorphic loci in 16Ap, which formed 3 haplotypes (16Ap-Hap1, 16Ap-Hap2 and 16Ap-Hap3). A CAPS (cleaved amplified polymorphic sequences) marker, 16Ap-Hap, was developed based on SNP to distinguish the promoter of TaCYP78A16-A alleles. A total of 3 genotypes were detected among the 30 wheat accessions and the 323 nature populations based on the CAPS marker. The functional marker, CAPS-16Ap, can be used in marker-assisted selection breeding in wheat.

Key words： Wheat Grain Weight Triticum aestivum cytochrome P450 78A16 (TaCYP78A16) SNP Cleaved amplified polymorphic sequences (CAPS) functional marker

Received: 17 April 2018 Published: 26 September 2018

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	Articles by authors
	WU Lin-Nan
	CI Wen-Ji
	GUO Li-Jian
	ZHANG Ya-Ting
	DIAO Hui-Xian
	LIU Xiang-Li
	MA Meng

Cite this article:

WU Lin-Nan,CI Wen-Ji,GUO Li-Jian, et al. Cloning and CAPS Marker Development of Seed Weight-Related Gene TaCYP78A16 in Wheat (Triticum aestivum)[J]. , 2018, 26(10): 1659-1669.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2018/V26/I10/1659

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