牛支原体生物被膜形成优势菌株的筛选及培养条件的优化

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Abstract Abstract Mycoplasma bovis (Mb) is an important pathogenic mycoplasma of cattle (Bos taurus). The biofilm formation of Mb can cause persistent infection in host cells and cause great economic loss to cattle industry. Therefore, the purpose of this study is to screen a dominant strain of Mb biofilm formation by identifying the biofilm formation ability of different Mb strains, and then optimize the cultural conditions on biofilm formation of Mb. First of all, the ability of biofilm formation of Mb PG45 strain, Hubei (HB) strain, Wuwei (WW) strain, Dingxi (DX) strain, and Lintao (LT) strain were identified by polystyrene microtiter plate assay, and then the strain with strong biofilm forming ability was used as the model strain to optimize the culture conditions of Mb biofilm formation. The results showed that the ability of biofilm formation was different among different strains of Mb, among which the biofilm formation ability of LT strain was the strongest, the next was HB strain. And the biofilm formation ability of PG45 strain, WW strain and DX strain was the weakest. The optimal cultural conditions for biofilm formation of Mb was that the Mb cultures (1.7E+08 CFU/mL) were inoculated at a dilution of 1∶10 into the Eaton medium containing 0.01 mg/mL fish sperm DNA, 20% serum of horse, 1% glucose and final pH 8.5, and then incubated at 37 ℃, with 5% CO2 for 72 h. Under the optimal cultural conditions, the biofilm formation ability of LT strain was sound. In this study, a dominant strain of Mb biofilm formation was obtained, and a stable and obvious biofilm was formed by optimizing the cultural conditions, which will provide excellent biomaterials for biofilm related study of Mb.

Key words： Mycoplasma bovis (Mb) Biofilm Dominant strain Influencing factors Optimization

Received: 04 April 2018 Published: 20 July 2018

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	Articles by authors
	GAO Xiang
	GENG Xiao-Yong
	FU Xiao-Beng
	YUN Feng-Qin
	XUE Hui-Wen
	WEI Pan-Meng
	FU Shi-Jun

Cite this article:

GAO Xiang,GENG Xiao-Yong,FU Xiao-Beng, et al. Dominant Strain Screening of Mycoplasma bovis in Biofilm Formation and Optimization of Cultural Conditions[J]. , 2018, 26(8): 1449-1456.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2018/V26/I8/1449

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