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| Coding Region Cloning and Expression Detection of GAL-1 Gene in Sika Deer (Cervus nippon) |
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Abstract The Galectin-1(GAL-1) gene in the deer antler stem cells is found to be highly expressed during antler regeneration period, indicating that it may be an important regulator for antler regeneration. To study the expression and possible biological function of GAL-1 in antler stem cells, antlerogenic periosteum and pedicle
periosteum(potentiated pedicle periosteum and dormant pedicle periosteum) and facial periosteum of sika
deer (Cervus nippon) were used as experimental materials. In this study, the GAL-1 gene CDS region of sika deer was cloned and bioinformatics analysis of GAL-1 was carried out using software or website. The relative expression of GAL-1 in the antler stem cells was detected by using Western-blot and qRT-PCR technique. The results showed that the GAL-1 ORF was 408 bp in length and encoded 134 amino acids. The relative molecular mass was 14.69 kD and the amino acid sequence was close to those of bovine (Bos taurus)and Ovis aries. GAL-1 was mainly distributed in the interstitial substance. It does not have transmembrane regions and glycosylation sites, and there may be two phosphorylation sites. The secondary structure of the GAL-1 protein was mainly β-sheets and random coil, and the tertiary structure was similar to that of the bovine. Expression level of GAL-1 was higher in the facial periosteum. In this study, the structural characteristics of GAL-1 protein was predicted, the expression level of GAL-1 protein in antler stem cells was detected and would provide the basic data for the further study of antler regeneration.
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Received: 20 August 2017
Published: 25 March 2018
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