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| Differences Expression of GH, IGF1 and Its Receptor Gene in Different Tissues of Mongolia Horse (Equus caballus) |
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Abstract Growth hormone (GH) has the ability to regulate the rhythm of growth. The present study was carried out to investigator the differential expression of GH, growth hormone receptor (GHR), insulin-like growth factor 1 (IGF1) and insulin-like growth factor 1 receptor (IGF1R) in different organs of Mongolia horse (Equus caballus). Four Mongolia horses with an average age of 2 years old were selected as the research object. The qRT-PCR and immunohistochemical detection were used to analyze the expression levels of GH, GHR, IGF1 and IGF1R in different organs of Mongolia horses. The results of RT-PCR results showed that the expression of GH in spleen were higher than in the lymph, heart, liver, kidney, skeletal muscle and tail muscle (P< 0.05). The expression of GHR in liver were higher than in the pancreas, tail muscle, testis, heart, skeletal muscle, lymph, kidney, spleen and lung (P< 0.05). The expression of IGF1 in liver were higher than in other organs (P<0.05). The expression of IGF1R in turn was lung, liver, spleen, testis, heart, kidney, lymph, skeletal muscle, tail muscle, pancreas (P<0.05). Unequal immunoreaction intensity was observed in different tissues by immunohistochemistry. According to the number of positive cells, the positive expression of GH protein in liver and spleen was higher than in other organs (P<0.05). It was found that the protein expression of IGF1 in liver was strongest, followed by the Spleen, kidney and testis, and that in skeletal muscle was weakest (P＜0.05). The present study showed that GH, GHR, IGF1, IGF1R had a differential expression in different tissues by studying at RNA level and protein level. This study provides a theoretical foundation and scientific basis for breeding of Mongolia horse.
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Received: 11 August 2017
Published: 25 March 2018
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