基于CRISPR系统基因编辑与基因调控技术的发展

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Abstract Bacteria and archaea have evolved an adaptive immune system, which was known as clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR -associated protein (Cas 9) system, and that uses short RNA to degrade the target sequences present in invading viral and phage DNAs. This system can identify the target sequence and cut the double-stranded DNA. So, it has been harnessed by thousands of laboratories for genome editing applications in a variety of experimental model systems since 2013. As a new type of genome editing techniques, the CRISPR/Cas9 system has advantages in simple in design, strong specificity and high efficiency. CRISPR/Cas9 system has brought a breakthrough in genome directional regulation and application, and rapidly applied in the basic theory, transgenic animal production, genetic diagnosis, clinical therapy fields. Whereas, every new technology has its limitations, CRISPR/Cas9 system also exists off-target effects and difficulty to insert genes. This limits its applications to some extent. Therefore, around the CRISPR system, many scientists improved a series of modification that can be used for the gene editing and gene expression of new tools in recent years. Here, we reviewed the research progress and application of new gene editing tools and gene expression modification based on the CRISPR system evolution and looking forward to the application prospect and development direction.

Key words： CRISPR Gene targeting Gene expression and regulation Artificial endonuclease Animal genetics and breeding

Received: 26 July 2017 Published: 04 February 2018

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	Articles by authors
	YANG Meng-Hui
	DAO Jing-Li
	CI Meng-Long
	WU Hao
	LIAN Zheng-Xin
	LIU Guo-Shi

Cite this article:

YANG Meng-Hui,DAO Jing-Li,CI Meng-Long, et al. Development of Genome Editing and Gene Regulation Using CRISPR System[J]. , 2018, 26(2): 183-193.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2018/V26/I2/183

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