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| Comparative Proteomic Analysis Between Biofilm-forming Cells and Planktonic Cells of Swine Brodetella Bronchiseptica |
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Abstract Bordetella bronchiseptica (Bb) is one of the pathogens of Swine infectious atrophic rhinitis (AR). Optimal conditions of Bb biofilm formation were developed, biofilm was well developed after 24 h, concentration of NaCl at 0.4%, pH 7.5, concentration of lactose at 0.5%. The protein expression profiles of whole Bb planktonic cells and biofilm-forming cells were investigated by comparative proteomics analysis, In this study, we compared the expression patterns of the whole Bb bacterial proteins originated from planktonic cells and biofilm-forming cells by a two-dimensional gel electrophoresis (2-DE). The results revealed that 15 protein spots were up-regulated and nine were down-regulated. Identified by mass spectrometry, the up-regulated protein spots mainly included elongation factor Tu (EF-Tu), superoxide dismutase (SOD) and molecular chaperone, involved in the stress, regulation and metabolism. The study is helpful to understand the Bb biofilm formation mechanism, and to prevent and control AR.
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Received: 05 April 2017
Published: 01 January 2018
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| [1]渠宏雁, 孟良玉, 刘锦峰,等.2013.副溶血性弧菌生物被膜的形成与优化[J]. 中国食品学报, 13(9):56-61. (Qu H Y,Meng L Y,Liu J F,et al.2013.Research on the Formation and Optimization of Vibrio Parahaemolyticus Biofilm[J].Journal of Chinese Institute of Food Science and Technology, 13(9):56-61.)[2]欧阳凤菊, 李兆利, 赫明雷,等.2010.禽致病性大肠杆菌生物被膜的形成及其影响因素[J]. 中国预防兽医学报, 11(9):672-676. (Ouyang F J,Li Z L,He M L,et al.2010.The biofilm forma tion of a via n pa thoge nic Es che richia coli in vitro[J].Chinese Journal of Preventive Veterinary Medicine, 11(9):672-676.)[3]Boles B R, Singh P K.2008. Endogenous oxidative stress produces diversity and adaptability in biofilm communities[J]. Proceedings of the National Academy of Sciences of the United States of America, 105(34):12503-8.[4]Boston R S, Viitanen P V, Vierling E.1996. Molecular chaperones and protein folding in plants[J]. Plant Molecular Biology, 32(1):191-222.[5]Droge W.2003.Oxidative stress and aging.[J]. Bangladesh Journal of Medical Science, 14(3):168–170.[6]Echeverri-toro L, Arango A, Ospina S, et al.2015. Bordetella bronchiseptica recurrent bacteraemia in a patient with bone marrow transplantation.[J]. Biomedica. 35(3): 302-305.[7]Gregorio E D, Franco M D, Martinucci M, et al.2015. Biofilm-associated proteins: news from Acinetobacter[J]. BMC Genomics, 16(1):1-14.[8]Hallstoodley L, Costerton J W, Stoodley P.2004. Bacterial biofilms: from the natural environment to infectious diseases.[J]. Nature Reviews Microbiology, 2(2):95-108.Henriette G, Serge M, Gerard A.,et al.2008. Autoinducer-2 Triggers the Oxidative Stress Response in Mycobacterium avium, Leading to Biofilm Formation[J]. Applied and environmental microbiology, 74(6):1798-804.[9]Irie Y, Mattoo S, Yuk M H.2004. The Bvg virulence control system regulates biofilm formation in Bordetella bronchiseptica.[J]. Journal of Bacteriology, 186(17):5692-5698.[10]Irie Y, Preston A, Yuk M H.2006. Expression of the primary carbohydrate component of the Bordetella bronchiseptica biofilm matrix is dependent on growth phase but independent of Bvg regulation.[J]. Journal of Bacteriology, 188(18):6680-6687.[11]Knox K W, Hardy L N, Markevics L J, et al.1985. Comparative studies on the effect of growth conditions on adhesion, hydrophobicity, and extracellular protein profile of Streptococcus sanguis G9B.[J]. Infection & Immunity, 50(2):545-54.[12]Levy-rimler G, Viitanen P, Weiss C, et al.2001. The effect of nucleotides and mitochondrial chaperonin 10 on the structure and chaperone activity of mitochondrial chaperonin 60[J]. European Journal of Biochemistry, 268(12):3465-3472.[13]Liu Y, Chen H, Wei Q, et al.2015. Immune efficacy of five novel recombinant Bordetella bronchiseptica proteins.[J]. BMC Veterinary Research, 11(1):1-7.[14]Mattoo S, Cherry J D.2005. Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due to Bordetella pertussis and Other Bordetella Subspecies[J]. Clinical Microbiology Reviews, 18(2):326-382.[15]Milev-milovanovic I, Long S, WILSONil M, et al.2006. Identification and expression analysis of interferon gamma genes in channel catfish.[J]. Immunogenetics, 58(1):70-80.[16]Pereira S F, JR Gonzalez R L, Dworkin J.2015. Protein synthesis during cellular quiescence is inhibited by phosphorylation of a translational elongation factor.[J]. Proceedings of the national academy of sciences of the united states of america. 112(25): E3274-E3281.[17]Rampelotto R F, Horner A, Horner C, et al.2016. Pneumonia caused by Bordetella bronchiseptica in two HIV-positive patients.[J]. SAO PAULO MEDICAL JOURNAL. 134(3): 268-272.Straw B E,Allaire S,Mengeling W L,et al.1999. Diseases of swine[M]. Ames,IA:Iowa State University Press, 670-680.[18]Tremoulet F, Duche O, Namane A, et al.2002. Comparison of protein patterns of Listeria monocytogenes grown in biofilm or in planktonic mode by proteomic analysis[J]. FEMS Microbiology Letters, 210(1):25-31. |
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