大口黑鲈LPL基因SNPs及其与适应人工配合饲料能力的相关性研究

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Abstract Largemouth bass (Micropterus salmoides) is a very important commercial farming fish with the annual production of more than 340 000 t in China. But it is a carnivorous teleost, and a large amount of forage fish and fish meal were used as food for largemouth bass every year. The residual forage fish and fish meal not only increase the culture costs, but also pollute waterbodies and farmlands seriously. In order to reduce culture costs and protect the natural environment, the selective breeding of the largemouth bass strain suitable for formulated feeds was carried out. It is a way to improve the effective conversion capability of largemouth bass for formulated feeds. In the previous study, the lipid levels of the formulated feeds have significant effects on the expression levels of 2 lipoprotein lipase genes (LPL type1 and LPL type2) in livers of largemouth bass. The report suggests the 1 genes potentially related to the adaptability of largemouth to formulated feed. In the current study, the primers of LPL genes were designed according to the known cDNA and the PCR amplifications were performed with largemouth bass genome DNAs as templates. After amplifying and sequencing, the genomic sequences of LPL type1 gene with the length of 6 170 bp were identified including 10 exons and 9 introns, and LPL type2 gene with the length of 4 419 bp were identified including 5 exons and 4 introns. By use of direct sequencing, eleven SNPs were detected in LPL type1 gene, and 6 SNPs were detected in LPL type2 gene. A total of 192 individuals from the cultured adult fish population fed on iced fresh fish and a total of 142 individuals from the cultured juvenile fish population fed on formulated feed were genotyped according to the SNP sites. The results showed that the 11 SNPs in LPL type1 were closely linked, and were named as T+156A. As well as the 6 SNPs in LPL type2 were closely linked, and named as C-224T. But the SNP sites were not closed linked between the 2 genes. The genotype frequency and gene frequency of the SNPs were similar between the 2 populations. And the calculations showed that the 2 populations were in Hardy-Weinberg equilibrium for the 2 SNPs. The general linear model was applied to analyze the relationship between SNPs and the full/empty traits, as well as the growth traits of largemouth bass. The results showed that there were no significant association between the LPL SNP sites and the growth traits of the adult fish fed on iced fish (P＞0.05). But in the juvenile fish population fed on formulated feed, the T+156A SNP was significantly related to the full or empty of the stomach and intestine. And the fish with AA genotype of T+156A SNP have significantly higher growth trait values in body weight and body length than those with TT genotype (P＜0.05). The results hinted that AA genotype in T+156A SNP has significant association with the adaptability of largemouth bass to the artificial diets. LPL type1 gene can be used as a candidate gene for the largemouth bass fed on formulated feeds, with high survival rate and fast growth rate. The current study not only provides the reliable research data to promote the breeding work of new strain of largemouth bass adaptable to formulated feed, but also provides the experiences and references for the bait domestication work of other carnivorous fish.

Key words： Largemouth bass（Micropterus salmoides） lipoprotein lipase artificial diets single nucleotide polymorphism (SNP)

Received: 14 June 2017 Published: 01 January 2018

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	Articles by authors
	MA Dong-Mei
	ZHU Yi-Min
	BO Jun-Jie
	QUAN Ying-Chun
	FAN Jia-Jia
	TIAN Wan-Wan
	LIAO Guo-Li
	LIANG Xu-Pang

Cite this article:

MA Dong-Mei,ZHU Yi-Min,BO Jun-Jie, et al. SNPs in LPL Genes and Study of Their Association Relationship With the Adaptability to Formulated Feed in Micropterus salmoides[J]. , 2018, 26(1): 140-149.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2018/V26/I1/140

Botstein D, white R L, Skolnick M, et al. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet, 32(3): 314-331.
Brookes A J. 1999. The essence of SNPs[J]. Gene, 234(2): 177-186.
Goldberg, I J. 1996. Lipoprotein lipase and lipolysis: central roles in lipoprotein metabolism and atherogenesis[J]. Journal of Lipid Research, 37, 693-707.
Gui L S, Jia C L, Zhang Y R, et al. 2016. Association studies on the bovine lipoprotein lipase gene polymorphism with growth and carcass quality traits in Qinchuan cattle[J]. Molecular and Cellular Probes, 30 (2): 61-65.
HALVER J E. 1988. Rsh Nutrition (second edition) [M]. Londen: Acad-nic Press. 185-200．
Li L, Liang X F, He S, et al. 2016. Genomic structure, tissue expression and single nucleotide polymorphisms of lipoprotein lipase and hepatic lipase genes in Chinese perch[J]. Aquaculture Nutrition, 22 (4): 786-800.
Ma Y Q, Thomas G N, Ng M C, et al. 2003. The lipoprotein lipase gene HindⅢ polymorphism is associated with lipid levels in early-onset type 2 diabetic patients[J]. Metabolism, 52(3): 338-343.
Mead J R, Irvine S A, Ramji D P. 2002. Lipoprotein lipase: structure, function, regulation, and role in disease[J]. Journal of Molecular Medicine, 80, 753-769.
Mo X, Liu X, Wang L, et al. 2013. Association of lipoprotein lipase polymorphism rs2197089 with serum lipid concentrations and LPL gene expression[J]. Journal of human genetics, 58 (3): 160-164.
Radha V, Vimaleswaran K S, Ashok Ayyappa K, et al. 2007. Association of lipoprotein lipase gene polymorphisms with obesity and type 2 diabetes in an Asian Indian population[J]. International Journal of Obesity, 31: 913–918.
Ren L, Ren X X. 2016. Meta-analyses of four polymorphisms of lipoprotein lipase associated with the risk of Alzheimer's disease[J]. Neuroscience Letters, 619: 73-78.
Scanu A. 1966. Serum high-density lipoprotein: effect of change in structure on activity of chicken adipose tissue lipase [J]. Science, 153(736): 640-641.
Wang L N, Yu Q, Xiong Y, et al. 2011. Lipoprotein lipase gene polymorphisms and risks of childhood obesity in Chinese preschool children[J]. European Journal of Pediatrics, 2011, 170 (10): 1309-1316.
白俊杰. 2013.我国大口黑鲈产业现状分析与发展对策[J]. 中国渔业经济, 31(5): 104-108. (Bai J J. 2013.Current status and development trend on China largemouth bass industry [J]. Chinese Fisheries Economics, 31(5): 104-108.
梁旭方, Oku H, Ogata H Y, 等. 2002. 海水鱼真鲷脂蛋白脂肪酶基因cDNA序列与组织表达[J]. 中国生物化学与分子生物学报, 6(6):712-719. (Liang X F, Oku H, Ogata H Y, et al. 2002. The cDNA Sequence and Tissue Expression of Lipoprotein Lipase Gene of a Marine Fish，Red Sea Bream (Pagrus major) [J]. Chinese Journal of Biochemistry and Molecular Biology, 6(6):712-719.)
吴锐全,黄樟翰,卢迈新. 2004. 大口黑鲈营养研究及配合饲料发展前景[J]. 广东饲料, 13(2): 38-39. (Wu R Q, Huang Z H, Lu M X. 2004. Nutritional Requirements of Largemouth Bass and Prospect of Its Commercial Feed[J]. Guangdong food, 13(2): 38-39.)
杨宇晖,梁旭方,方荣,等. 2011. 鳜脂蛋白脂酶基因SNP及其与食性驯化相关性分析[J].遗传, 9 (33): 996-1002. (Yang Y H, Liang X F, Fang R, et al. 2011. Polymorphism of SNPs in the lipoprotein lipase (LPL) in Siniperca chuatsi and their association with feed habit domestication)
姚煜, 梁旭方, 李光照, 等. 2009. 鳜脂蛋白脂酶和肝脂酶基因结构与组织表达[J]. 中国水产科学, 16(4): 506-517. (Yao Y, Liang X F, Li G Z, et al. 2009. Genomic structure and expression of lipoprotein lipase (LPL) and hepatic lipase (HL) genes in Siniperca chuatsi[J]. Journal o f Fishery Sciences of China, 16(4): 506-517.)
朱择敏,马冬梅,白俊杰,等. 2013. 大口黑鲈两种脂蛋白脂肪酶基因cDNA的克隆及表达特征分析[J]. 水产学报, 37(5):1-10.（Zhu Z M, Ma D M, Bai J J, et al. 2013. Cloning and expression analysis of two cDNAs encoding lipoprotein lipase of Micropterus salmoides[J]. Journal of Fisheries of China, 37(5):1-10.）
朱择敏,马冬梅,白俊杰,等. 2014. 配合饲料、冰鲜杂鱼对大口黑鲈生长和LPL基因mRNA表达的影响[J]. 大连海洋大学学报, 29(4):360-363.（Zhu Z M, Ma D M, Bai J J, et al. 2014. Effects of formulated diets and frozen trash fish on growth and expression of LPL gene mRNA in largemouth bass Micropterus salmoides[J]. Journal of Dalian Fisheries University, 29(4):360-363.）