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| Single Nucleotide Polymorphism of Apolipoprotein Genes and Analysis of Its Correlation with Growth Traits in Micropterus salmoides |
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Abstract Abstract：In this study, the primers was designed according to cDNA sequences of apolipoprotein gene from EST database of largemouth bass and the genomic fragments of Apo-A1, Apo-A4 and Apo-C1 were amplified, respectively. 4 single nucleotide mutations were identified by using directly sequencing. The A+489C mutation was located in Apo-A1 gene. The A+633T mutation was located in Apo-A4 gene. The two mutations (A+24G and A+75C) were located in Apo-C1 gene. The genotype of each SNP in 159 largemouth bass was analyzed with the method of SnaPshot. Chi-square test showed that four SNP loci were in Hardy-Weinberg equilibrium state in the largemouth bass population. A+633T, A+24G and A+75C had the same genotype frequency, which formed two haplotypes (A and B) and constituted with three genotypes (AA, AB, and BB). This suggested that the three SNPs might completely link. A general linear model was used to analyze the relationship between polymorphisms of apolipoprotein gene and major growth traits. The result indicated that A+489C site showed significant association with body weight and body high (P<0.05). The different genotypes of A+633T in Apo-A4, A+24G and A+75C sites in Apo-C1 genes showed significant association with body weight, body high and total length, respectively (P<0.05). Our results showed that 4 SNPs had genetic effects on the growth traits in largemouth bass, which could be important candidate molecular markers for largemouth bass molecular marker-assisted selection.
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Received: 20 April 2017
Published: 01 January 2018
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