|
|
|
| Biological Characteristics of Two H3N1 Subtype Avian influenza viruses (AIV) |
|
|
|
|
Abstract H3N1 subtype Avian influenza virus (AIV) can infect wild birds, poultry, pigs (Sus scrofa) and dogs (Canis lupus familiaris). In order to understand the biological property of H3N1 subtype AIV, phylogenetic analysis and specific pathogen free (SPF) animal infection experiments were conducted for two H3N1 strains, A/environment/Anhui/S4108/2011 (EN/AH/S4108/2011) and A/duck/Fujian/S2196/2012 (DK/FJ/S2196/2012) which were isolated from Anhui and Fujian province in 2011 and 2012, respectively. The phylogenetic analysis showed that the genetic origin of the two stains were obviously diversified. The neuraminidase (NA) and matrix protein (M) genes of DK/FJ/S2196/2012 and the polymerase basic protein 1 (PB1), NA, and non-structural protein (NS) genes of EN/AH/S4108/2011 were most closely related to those of H5N1 AIVs. Besides, these two viruses shed only through oropharyngeal route of infected chickens (Gallus gallus) and didn't have the ability to horizontal transmission. The two viruses could efficiently replicate in the nasal turbinates and lungs of infected mice (Mus musculus) without prior adaptation. In addition, these two viruses did not cause any obvious clinical symptoms of infected mice, and thereby they were low pathogenic to mice. In conclusion, two H3N1 viruses showed genetic diversity and complexity in their origin and had a low potential risk to infect mammals. This study indicated that H5N1 AIVs in eastern China were involved in reassortment with H3N1 subtype AIVs genome, which provides scientific basis for prevention and control of H3N1 subtype AIVs.
|
|
Received: 07 February 2017
Published: 16 June 2017
|
|
|
|
|
|
| [1]Campitelli, L.Fabiani,C,Puzelli,S.,et al.,H3N2 influenza viruses from domestic chickens in Italy: an increasing role for chickens in the ecology of influenza?[J].Journal of General Virology, 2002, 83(2):413-420
[2]Choi, J.G.,Kang,H-M.,Kim,M.-C.,et al.,Genetic relationship of H3 subtype avian influenza viruses isolated from domestic ducks and wild birds in Korea and their pathogenic potential in chickens and ducks[J].Veterinary microbiology, 2012, 155(2-4):147-157
[3]Choi, Y.K.,Seo,SH.,Kim,J.A.,et al.,Avian influenza viruses in Korean live poultry markets and their pathogenic potential[J].Virology, 2005, 332(2):529-537
[4]Deng, G.Tan,D,Shi,J.,et al.,Complex reassortment of multiple subtypes of avian influenza viruses in domestic ducks at the Dongting Lake region of China[J].Journal of Virology, 2013, 87(17):9452-9462
[5]Lee, H.J.,Kwon,J-S.,Lee,D.-H.,et al.,Continuing evolution and interspecies transmission of influenza viruses in live bird markets in Korea[J].Avian diseases, 2010, 54(1):738-748
[6]Lekcharoensuk, P.Lager,KM.,Vemulapalli,R.,et al.,Novel swine influenza virus subtype H3N1,United States[J].Emerg Infect Dis, 2006, 12(5):787-794
[7] Li, X.Co-circulation of H5N6, H3N2, H3N8, and Emergence of Novel Reassortant H3N6 in a Local Community in Hunan Province in China. Reassortant H3N6 in a Local Community in Hunan Province in China[J]. Scientific Reports, 2016, 6: 25549.[J].Scientific Reports, 2016, 6(25549):1-8
[8]Liu, M.He,S,Walker,D.,et al.,The influenza virus gene pool in a poultry market in South central china[J].Virology, 2003, 305(2):267-275
[9] Moreno, A.[J].Veterinary microbiology, 2009, 138(3-4):361-367
[10]Peng, Y.Xie,Z-x.,Liu,J.-b.,et al.,Epidemiological surveillance of low pathogenic avian influenza virus (LPAIV) from poultry in Guangxi Province,Southern China[J].PLoS One, 2013, 8(10):e77132-7
[11]Pu, J.Zhang,G-z.,Ma,J.-h.,et al.,Serologic evidence of prevalent avian H3 subtype influenza virus infection in chickens[J].Avian diseases, 2009, 53(2):198-204
[12]Qiu, B.Liu,W,Peng,D.,et al.,Distribution of avian influenza virus subtypes among domestic ducks in eastern China[J].Wei sheng wu xue bao= Acta microbiologica Sinica, 2008, 48(10):1290-1294
[13]Siddique, N.Naeem,K,Ahmed,Z.,et al.,Isolation,identification,and phylogenetic analysis of reassortant low-pathogenic avian influenza virus H3N1 from Pakistan[J].Poultry science, 2012, 91(1):129-138
[14]Song, D.Moon,H-J.,An,D.-J.,et al.,A novel reassortant canine H3N1 influenza virus between pandemic H1N1 and canine H3N2 influenza viruses in Korea[J].Journal of General Virology, 2012, 93(3):551-554
|
|
|
|
