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Abstract Epsilon - lycopene cyclase (LCYE) is involved in synthesis of the key enzymes of the carotenoid and plays important roles in carotenoid synthesis. In order to explore Hibiscus esculentus LCYE in gene function, In this study, the full-length cDNA sequence of lycopene epsilon-cyclase (LCYE) gene that encoded carotenoid biosynthesis downstream enzymes lycopene epsilon-cyclase was isolated from H. esculentus. by RT-PCR and RACE (rapid-amplification of cDNA ends) technique. The cDNA sequence was 1 845 bp in length and consisted of a 1 605 bp ORF that encoded a polypeptide of 534 amino acids, with a predicted molecular weight (MW) of 59.73 kD and a hypothetical isoelectric point (pI) of 6.22. The total average hydrophobicity (GRAVY) of the LCYE protein was 0.036, and it was predicted as hydrophobic protein. It shared over 80% identity with the homologous proteins from Gossypium arboretum, Theobroma cacao and Jatropha curcas, proving that it was highly conservative. This gene was named HyLCYE, and the GenBank accession was KX257999. The fluorescent quantitative analysis indicated that the expression level of HyLCYE gene was the highest in mature leaves and the lowest in roots. During the development of leaves, the highest expression level was found in mature leaves and the lowest in old leaves. During the development of fruits, the highest expression level was found in 3 d after flowering and the lowest in 1 d after flowering. The ultra performance liquid chromatography (UPLC) analysis showed that lutein contents were the highest in mature leaves and 3 d after flowering. These results suggested that the expression level of LCYE was positively correlated with lutein contents. The total carotenoid contents among different genotypes okra varieties were highest in green varieties, followed by red varieties, and was lowest in white varieties. The results provide a scientific base for the further development and utilization of Hibiscus esculentus.
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Received: 17 March 2017
Published: 30 October 2017
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