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Abstract Epsilon - lycopene cyclase (LCYE) is involved in synthesis of the key enzymes of the carotenoid and plays important roles in carotenoid synthesis. In order to explore Hibiscus esculentus LCYE in gene function, In this study, the full-length cDNA sequence of lycopene epsilon-cyclase (LCYE) gene that encoded carotenoid biosynthesis downstream enzymes lycopene epsilon-cyclase was isolated from H. esculentus. by RT-PCR and RACE (rapid-amplification of cDNA ends) technique. The cDNA sequence was 1 845 bp in length and consisted of a 1 605 bp ORF that encoded a polypeptide of 534 amino acids, with a predicted molecular weight (MW) of 59.73 kD and a hypothetical isoelectric point (pI) of 6.22. The total average hydrophobicity (GRAVY) of the LCYE protein was 0.036, and it was predicted as hydrophobic protein. It shared over 80% identity with the homologous proteins from Gossypium arboretum, Theobroma cacao and Jatropha curcas, proving that it was highly conservative. This gene was named HyLCYE, and the GenBank accession was KX257999. The fluorescent quantitative analysis indicated that the expression level of HyLCYE gene was the highest in mature leaves and the lowest in roots. During the development of leaves, the highest expression level was found in mature leaves and the lowest in old leaves. During the development of fruits, the highest expression level was found in 3 d after flowering and the lowest in 1 d after flowering. The ultra performance liquid chromatography (UPLC) analysis showed that lutein contents were the highest in mature leaves and 3 d after flowering. These results suggested that the expression level of LCYE was positively correlated with lutein contents. The total carotenoid contents among different genotypes okra varieties were highest in green varieties, followed by red varieties, and was lowest in white varieties. The results provide a scientific base for the further development and utilization of Hibiscus esculentus.
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Received: 17 March 2017
Published: 30 October 2017
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[1]Kim Y S, Park S J, Cho YH, Park J,.Efflect of combined treatment of high hydrostatic pressure and mild heat on the quality of carrot juice[J].Journal of Food Science, 2011, 66(9):1355-1360[2]Zanfini A, Corbini G, La Rosa C, Dreassi E, .Antioxidant activity of tomato lipophilic extracts and interactions between carotenoids and a-tocopherol in synthetic mixtures[J].LWT-Food Scienee andTechnology, 2010, 43(0):67-72[3]Aruna G, Baskaran V.Comparative study on the levels ofcarotenoids lutein,zeaxanthin andβ-carotene in Indian spices of nutritional and medicinal importance.[J].Food Chemistry, 2010, 123(2):404-409[4]Semba R D, Lauretani F, Ferrucci L.Carotenoidsas protection against sarcopenia in older adults[J].Archives of Biochemistry and Biophysics, 2007, 458(2):141-145[5]Oliver J, Palou A, 2000.Hromatographic determination of carotenoids in foods. [J].Journal of Chromatography A, 2000, 881(1/2):543-555[6]Bone R A, Landrum J T, Fernandez L, Tarsis S L.Analysis of the macular pigment by HPLC: retinal distribution and age study[J].Investigative Ophthalmology & Visual Science, 1988, 29(6):843-849[7]Bone R A, Landrum J T, Hime G W.Stereochemistry of the hum an macular carotenoids[J].Investigative Ophthalmology & Visual Science, 1993, 34(6): 2033-2040[8]Elizabeth J, Johnson.The Role of Carotenoids in Human Health[J].Nutrition in Clinical Care, 2002, 5(2): 56-65[9]Chew BP, Park JS.Carotenoid action on the immune response[J].Journal of Nutrition, 134(1): 257S-261S, 2004, 134(1):257S-261S[10]李大婧, 刘春泉, 白云峰, 方桂珍.叶黄素、玉米黄质研究进展--叶黄素、玉米黄质的结构、性质和生物学功能[J].核农学报, 2006, 20(1):64-67(1):64-67[11]Harjes C E, Rocheford T R, Bai L, Brutnell TP, Kandianis C B, Sowinski, S G, Stapleton A E, Vallabhaneni R, Williams M, Wurtzel E T, Yan J, Buckler E S.Natural genetic variation in lycopene epsilon cyclase tapped for maize biofortification.[J].Science, 2008, 319(1):330-333[12]Clotault J, Peltier D, Berruyer R, Thomas M, Briard M, Geoffriau E.Expression of carotenoid biosynthesis genes during carrot root development[J].Journal of Experimental Botany, 2008, 59(13): 3563-3573.[13]Ampomah-Dwamena C, Dejnoprat S, Lewis D, Sutherland P, Volz RK, Allan A C.Metabolic and gene expression analysis of apple (Malus x domestica) carotenogenesis[J].Journal of Experimental Botany, 2012, 63(12):4497-4511[14]Kato M, Ikoma Y, Matsumoto H, Sugiura M, Hyodo H, Yano M.Accumulation of carotenoids and expression of carotenoid biosynthetic genes during maturation in Citrus fruit[J].Plant Physiology, 2004, 134(2): 824-837[15](黄阿根, 陈学好, 高云中, 车婧.黄秋葵的成分测定与分析[J].食品科学, 2007, 28(10):451-451[16]王君耀, 周峻, 汤谷平.黄秋葵抗疲劳作用的研究[J].中网现代应用药学杂志, 2003, 20(4):316-317[17]张慧, 李涛, 徐公世.一种前景广阔的天然食品着色剂-叶黄素., 5: 45-48)[J].中国食品添加剂, 2004, 5(0):45-48[18]侯冠彧, 刘国道, 王东劲. 日粮中添加黄秋葵茎叶粉对大麻花鸡皮肤及脂肪着色的影响[J].中国农学通报, 2006, 22(2):11-13[19]刘国道, 王东劲, 侯冠彧, 陈雅芳. 黄秋葵茎叶粉对文昌鸡蛋黄着色的影响[J].中国农学通报, 2006, 22(7):16-19[20]吴佳静, 周汉林, 荣光, 刘国道, 吴滴峰.黄秋葵叶粉对海兰褐蛋鸡部分生产性能及蛋品质的影响[J].热带作物学报, 2012, 33(4):755-758[21]Dwyer J H, Navab M, Dwyer K M, Hassan K, Sun P, Shircore A, Hama-Levy S, Hough G, Wang X, Drake T, Merz C N, Fogelman A M.Oxygenated carotenoid lutein and progression of early atherosclerosis [J].Circulation, 2011, 103(24):2922-927[22]Granado F, Olmedilla B, Blanco L.Nutritional and clinical relevance of lutein in human health[J].BritishJournal of Nutrition, 2003, 90(3): 487-502[23]刘东祥, 叶花兰, 刘国道. 14个黄秋葵品系叶黄素和β-胡萝卜素产量比较试验[J].中国农学通报, 2006, 22(6):425-427[24]Cunningham F X J, Gantt E.One ring or two Determination of ring number in carotenoids by lycopene epsilon-cyclases[J].Proceedings of the National Academy of Sciences of the United States of America, 2001, 98(5): 2905-2910[25]王慧, 欧承刚, 庄飞云, 赵志伟, 马振国.胡萝卜中类胡萝卜素积累与主要合成基因转录水平相关性分析[J].园艺学报, 2014, 41(12):2513-2520[26]Moehs C P, Tian L, Osteryoung K W, DellaPenna D.Analysis of carotenoid biosynthetic gene expression during marigold petal development [J].Plant Molecular Biology, 2004, 45(3): 281-293[27]Del Villar-Martinez A A, Garcia-Saucedo P A, Carabez-Trejo A, Cruz-Hemandez A, Paredes-Lopez O.Carotenogenic gene expression and ultrastructural changes during development in marigold. [J].Journal of Plant Physiology, 2005, 162(9):1046-1056[28]Pogson B J, Rissler H M.Genetic manipulation of carotenoid biosynthesis and photoprotection[J].Philosophical transactions - Royal Society. Biological sciences, 2000, 355(1402): 1395-1403[29]Singhd,Tripathi R D,Singhhn.Effect of age of picking on the chemical composition of the fruits of Okra[J].Indian Journal of Agricultural Science, 1974, 44(1):22-26[30]许如意, 罗丰, 袁廷庆, 黄仁功, 李劲松.不同采摘期对黄秋葵果实性状和品质的影响[J].长菜江蔬菜, 2011, 01(0):18-20 |
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