|
|
|
| Adaptability of Rice (Oryza sativa) Varieties to Cold Tolerance Associated with Methylation Variation of ICE1 Gene |
|
|
|
|
Abstract Abstract Rice (Oryza sativa) is originated from the tropics, but human is continuously exploring its suitable planting areas northward, thus, the selection of cold-tolerant rice cultivars is required. However, the studies on the mechanism of differentiation on cold tolerance among rice cultivars has not been documented. In the present study, three materials of rice germ from different climate regions in Liaoning, Jiangsu and Guangdong provinces in China were selected. Their cold-tolerance was comparatively determined by indexes of cold injury and chlorophyll florescent. Methylation sites of coding and promoter regions of ICE1 (inducer of CBF expression 1) in CBF(C-repeat binding factor) cold response transcription pathway were determined to elucidate molecular mechanism of cold differentiation in different regions using DNA methylation sequencing sulfite (bisulfite sequencing PCR, BSP). The results showed that the differences in methylation levels of ICE1 gene were only found at promoter regions(P＜0.05). Danjing-17 rice cultivar from Liaoning (LN004) had 2 cytosine methylations at ICE1 gene promoter region and the highest index of cold tolerance; Nanjing-5055 from Jiangsu (JS013) had 7 cytosine methylations, and the middle index. Yuexinzhan-2 from Guangdong (GD008) had 11 cytosine methylation and the the lowest index. qRT-PCR tests indicated that ICE1 showed significant positive correlation with downstream regulated expression of CBF1 (C-repeat DRE binding factor 1) and CBF3 (C-repeat DRE binding factor 3) on cold tolerance level (P＜0.05), but ICE1 showed significant negative relationship with the ICE1 methylation levels(P＜0.05). These indicated that cold tolerance among 3 rice cultivars may be adapted to local climate, and were regulated by CBF cold response transcription pathway, which was determined by the methylation of ICE1 gene. This study may provides theoretical basis for further explanation of the molecular mechanism of cold tolerance in rice.
|
|
Received: 13 March 2017
Published: 06 August 2017
|
|
|
|
| [1]俊枝, 周雷, 潘英华, 姚国新.任银玲耐冷调节基因及其在植物耐冷基因工程中的应用新进展[J].作物杂志, 2014, 10(6):16-16
[2]harifi P.Evaluation on sixty-eight rice germplasms in cold tolerance at germination stage[J].Rice Science, 2010, 17(1):77-81
[3]aruah, AR.Ishigo-Oka N,Adachi M,Oguma Y,Tokizono Y,Onishi K,Sano YCold tolerance at the early growth stage in wild and cultivated rice[J].Euphytica, 2009, 165(3):459-470
[4]黄力士, 刘凌峰, 刘之熙.水稻耐冷性研究进展与前景[J]. 湖南农业科学, 2008 (1): 3-6.
[5]aek JS, Chung NJ.Seed wintering and deterioration characteristics between weedy and cultivated rice[J].Rice, 2012, 5(1):21-
[6]heng C, Yun KY, Ressom HW, Mohanty B, Bajic VB, Jia Y, Song JY, Reyes BG.An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice[J].BMC Genomics, 2007, 8(1):175-
[7]hinnusamy V, Zhu J, Zhu JK.Cold stress regulation of gene expression in plants[J].Trends in Plant Science, 2007, 12(10):444-451
[8]ee BH, Zhu JK.The Arabidopsis cold-responsive transcriptome and its regulation by ICE1[J].Plant Cell, 2005, 17(11):3155-75
[9]ie HJ, Li H, Liu D, Dai.WM,He JY,Lin S,Duan H,Liu LL,Chen SG,Song XL,Valverde BE,Qiang SICE1 demethylation drives the range expansion of a plant invader through cold tolerance divergence[J].Molecular Ecology, 2015, 24(4):835-850
[10]卓婉清, 腊红桂.拟南芥甲基化及去甲基化研究进展[J].南方农业, 2015, 9(9):129-133
[11]李娜, 张旸, 解莉楠, 等.植物甲基化研究进展[J].植物生理学报, 2012, 48(11):1027-1036
[12]史玉杰, 李庆贺, 刘晓辉.甲基化与基因表达调控研究进展[J].中国生物工程杂志, 2013, 33(7):90-96
[13]黑淑梅, 慕明涛.甲基化在植物生长发育中的作用[J].安徽农业科学, 2007, 35(21):6368-6369
[14]Steward N, Ito M, Yamaguchi Y, Koizumi N, Sano H, Periodic DNA methylation in maize nucleosomes and demethylation by environmental stress [J].Journal of Biological Chemistry, 2002, 277 (40): 37741-37746.
[15]Li XL, Lin ZX, Nie YC, Guo XP, Zhang XL.MSAP analysis of epigenetic changes in cotton (Gossypium hirsutum L) under salt stress[J].Acta Agronomica Sinica, 2009, 35(4):588-596
[16]Szyf M.DNA methylation,behavior and early life adversity[J].Journal of Genetics and Genomics, 2013, 40(7):331-338
[17]杨美娜, 杨瑰丽, 郭涛, 刘永驻, 张建国, 陈志强, 王慧.逆境胁迫下植物甲基化及其在抗旱育种中的研究进展[J].中国农学通报, 2013, 29(6):6-11
[18]Gilmour S.Sebolt AM,Salazar MP,Everard JD,Thomashow MFOverexpression of the Arabidopsis CBF3 transcriptional activator mimics multiple biochemical changes associated with cold acclimation[J].Plant Physiology, 2000, 124(4):1854-1865
[19]刘丹.外来入侵植物紫茎泽兰冷驯化相关基因ICE1的克隆[D]. 南京: 南京农业大学, 2010.
[20]Genty B, Briantais JM, Baker NR, Baker NR.The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J].Biochimica et Biophysica Acta, 1989, 990(1):87-92
[21]饶玉春, 杨窑龙, 黄李超, 潘建伟, 马伯军, 钱前, 曽大力.水稻耐冷胁迫的研究进展[J].分子植物育种, 2013, 11(3):443-450
[22]Ibarra CA, Feng X, Schoft VK, Hsieh T, Uzawa R, Rodrigues JA, Zilberman D.Active DNA demethylation in plant companion cells reinforces transposon methylation in gametes[J].Science, 2012, 337(6100):1360-1364
[23]Gent JI, Ellis NA, Guo L, Harkess A, Yao Y, Zhang X, Dawe RK.CHH islands: de novo DNA methylation in near-gene chromatin regulation in maize[J].Genome research, 2013, 23(4):628-637
[24]Shibukawa T, Yazawa K, Kikuchi A, Kamada H.Possible involvement of DNA methylation on expression regulation of carrot LEC1 gene in its 5'-upstream region[J].Gene, 2009, 437(1-2):22-31
[25]王瑞娴, 徐建红.基因组甲基化及组蛋白甲基化[J].遗传, 2014, 36(3):191-199
[26]南楠, 曾凡锁, 詹亚光.植物甲基化及其研究策略[J].植物学报, 2008, 25(1):102-111
[27]张晗, 信月芝, 郭惠明, 程红梅.转录因子及其在植物抗冷反应中的作用[J].核农学报, 2006, 20(5):406-409
|
|
|
|
