|
|
|
| SNPs Analysis of LAMB1 Gene in Fine-wool Sheep (Ovis aries) Based on the DNA Pools and Re-sequencing Technologies |
|
|
|
|
Abstract Laminin-beta-1 (LAMB1) gene is the member of the laminin family, and plays an important role in biological processes. To analyze the genetic effects associated with the wool traits of candidate genes LAMB1, based on DNA pools with the re-sequencing technology to screened 5 single nucleotide polymorphisms (SNPs) data of LAMB1 gene exon regions, combining with single strand conformation polymorphism analysis of polymerase chain reaction products (PCR-SSCP) technology to type the genotype, the genetic effects of LAMB1 gene 5 SNPs on wool traits of totally 418 sheep (Ovis aries) in fine-wool sheep at Xinjiang Kunes farm were analyzed by the GLM of SAS 8.1. Linkage disequilibrium (LD) was used by HaploView 4.2. The results showed this population genetic variations of fine-wool sheep was in the medium level, there were 5 non-synonymous mutation SNPs. AA and GA genotype in the identification of the body weight and body weight after shearingk of the individual value was extremely significant higher than GG genotype on SNP2 (P＜0.01). CC, TT and TC genotypes in the staple length of the individual value was extremely significant on SNP4(P＜0.01). CC genotype in the body weight after shearingk of the individual value was extremely significant higher than TT and TC genotype on SNP4 (P＜0.01). While, each genotypes in the part of wool traits on other SNPs were significance (P＜0.05). SNP1 and SNP3, SNP2 and SNP3 were in the linkage disequilibrium state, LAMB1 gene might be used as one of a potential and valuable candidate molecular marker on wool traits in fine-wool sheep. The study revealed the molecular genetic features of LAMB1 gene and evaluated the genetic relationship in fine-wool sheep. The results provide molecular genetic basis for efficient breeding of fine-wool sheep breeds, and efficient conservation and utilization the germplasm resources.
|
|
Received: 26 May 2016
Published: 07 November 2016
|
|
|
|
| [1]陈强.MIR-124-5P对LAMB1转录后调控作用抑制高级别胶质瘤的生长. 南方医科大学, 2014.
[2]杜雪莲.基因芯片检测子宫内膜癌放射敏感性及血管生成相关基因的初步研究. 山东农业大学, 2009.
[3]田月珍.细毛羊母羊羊毛细度相关候选基因的筛选及其关联性分析. 新疆农业大学, 2012.
[4]狄江，拉扎提·艾尼瓦尔，徐新明，等.不同羊毛纤维直径细毛羊皮肤组织差异表达基因研究[J].畜牧兽医学报, 2013, 44(5):681-689
[5]张蔓丽，卢彦平，李亚里.初级纤毛与信号通路相关性研究进展[J].遗传, 2015, 37(3):233-239
[6]张莉.绵羊肉用性状全基因组关联分析. 中国农业科学院, 2013.
[7]初芹， 李东， 侯诗宇，等.基于池测序法筛选奶牛高信息量标记的可行性[J].遗传, 2014, 36(7):691-696
[8]Svensson ME, Johannesson H, Engstrom P.The LAMB1 gene from the clubmoss,Lycopodium annotinum,is a divergent MADS-box gene,expressed specifically in sporogenic structures[J].Gene, 2000, 253(1):31-43
[9]Mats ES, Peter E.Closely related MADS-box genes in club moss (Lycopodium) show broad expression patterns and are structurally similar to,but phylogenetically distinct from,typical seed plant MADS-box genes[J].New Phytologist, 2002, 154(2):439-450
[10]Leena P, Donald RG, Angela MC, et al.Cloning of the β3 chain gene (LAMB3) of human laminin 5,a candidate gene in junctional epidermolysis bullosa[J].Genomics, 1995, 25(1):192-19
[11]Hutcheson H, Olson L, Bradford Y, et al.Examination of NRCAM,LRRN3,KIAA0716,and LAMB1 as autism candidate genes[J].BMC Medical Genetics, 2004, 5(1):12-13
[12]Hopker VH, Shewan D, Tessier L M, et al.Growth-cone attraction to netrin-1 is converted to repulsion by laminin-1[J].Nature, 1999, 401(6748):69-73
[13]Schlake T.Determination of hair structure and shape[J].Seminars in cell and developmental Biology, 2007, 18(2):267-273
[14]Brancaccio A, Minichiello A, Grachtchouk M, et al.Requirement of the forkhead gene Foxe1,a target of sonic hedgehog signaling,in hair follicle morphogenesis[J].Human Molecular Genetics, 2004, 13(21):2595-2606
[15]Rasal KD, Shah TM, Vaidya M, et al.Analysis of consequences of non-synonymous SNP in feed conversion ratio associated TGF-β receptor type 3 gene in chicken. [J].Meta Gene, 2015, 4:107-117
[16]Dang YL, Li MX, Yang MJ, et al.Identification of bovine NPC1 gene c SNPs and their effects on body size traits of Qinchuan cattle[J].Gene, 2014, 540(2):153-160
[17]Vage DI, Husdal M, Kent MP, et al.A missense mutation in growth differentiation factor 9 (GDF9) is strongly associated with litter size in sheep[J].BMC Genetics, 2013, 14(1):1471-2156
[18]Shota N, Toshio W, Kazunori M, et al.Genome-wide association study identified three major QTL for carcass weight including the PLAG1-CHCHD7 QTN for stature in Japanese Black cattle[J].BMC Genetics, 2012, 13(1):40-47
[19]Kuno SI.Division of linkage disequilibrium between absolute linkage disequilibrium and linkage equilibrium[J].Journal of Human Genetics, 2005, 50(6):315-316
[20]Kumar A, Agarwal S, Pradhan S.Haplotype analysis and LD detection at DML locus[J].Gene, 2015, 567(1):45-50
|
|
|
|
