应用锁式探针技术检测大豆细菌性斑疹病

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Abstract Bacterial pustule is a serious seed-borne disease, which dramatically reduces soybean(Glycine max) yield. The disease becomes more and more serious around the country these years. This research selected house-keeping gene of DNA replication and repair protein (recF) as target gene, and developed a bacterial pustule detection system based on padlock probe combined with dot-blot hybridization. The results indicated that the Padlock probe could detect the bacterial pustule pathogen specifically, and its sensitivity could be up to 100 fg/μL. The system also facilitated the detection of Xanthomonas axonopodis pv. glycines in soybean seedlots with 0.1% artificially infested seed. On the other hand, 45 samples of commercial soybean seeds were detected by using this method, and detected 4 samples positive with the bacterial pustule pathogen. The results indicated that the detecting system based on padlock probe combined with dot-blot hybridization could detect Xanthomonas axonopodis pv. glycines accurately, and provide useful references for prevention and control of bacterial pustule.

Key words： Padlock probe Bacterial pustule of soybean Detection DNA replication and repair protein gene (recF)

Received: 30 March 2015 Published: 24 July 2015

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	Articles by authors
	CHEN Yan-Hong
	TIAN Yan-Li
	DIAO Yu-Jiang
	HU Bai-Shi

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CHEN Yan-Hong,TIAN Yan-Li,DIAO Yu-Jiang, et al. Development of Padlock Probe Based Method for Detection of Bacterial Pustule of Soybean (Glycine max)[J]. , 2015, 23(10): 1377-1385.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2015/V23/I10/1377

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