Abstract Bacterial pustule is a serious seed-borne disease, which dramatically reduces soybean(Glycine max) yield. The disease becomes more and more serious around the country these years. This research selected house-keeping gene of DNA replication and repair protein (recF) as target gene, and developed a bacterial pustule detection system based on padlock probe combined with dot-blot hybridization. The results indicated that the Padlock probe could detect the bacterial pustule pathogen specifically, and its sensitivity could be up to 100 fg/μL. The system also facilitated the detection of Xanthomonas axonopodis pv. glycines in soybean seedlots with 0.1% artificially infested seed. On the other hand, 45 samples of commercial soybean seeds were detected by using this method, and detected 4 samples positive with the bacterial pustule pathogen. The results indicated that the detecting system based on padlock probe combined with dot-blot hybridization could detect Xanthomonas axonopodis pv. glycines accurately, and provide useful references for prevention and control of bacterial pustule.
CHEN Yan-Hong,TIAN Yan-Li,DIAO Yu-Jiang, et al. Development of Padlock Probe Based Method for Detection of Bacterial Pustule of Soybean (Glycine max)[J]. , 2015, 23(10): 1377-1385.
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