|
|
|
| The Influence of Different Ns Chromosomes in Wheat- Psathyrostachys huashanica Disomic Addition Lines on Young Ear Development and Photosynthesis |
|
|
|
|
Abstract In wheat breeding, young ear development process and photosynthesis are two factors which must be considered. In this study, a complete set of wheat-Psathyrostachys huashanica disomic alien addition lines were studied on young ear development process and photosynthesis. The purpose of this article is to map associated genetic traits on chromosome, and comprehensive evaluation of the young ear development process and photosynthetic effect of Psathyrostachys huashanica alien chromosomes. The sequenced characterized amplified region (SCAR) maker identification showed that all of this wheat-Psathyrostachys huashanica disomic alien addition lines had Psathyrostachys huashanica chromosomes. Anatomic observation indicated that the young ear of the addition line of 6Ns developed fastest, and the mature period shortened 14~16 days compared with parents 7182; the development rate of the 5Ns and 2Ns addition line were behind, and the mature period of the 4Ns addition line were 6~7 days later compared with parents 7182. The photosynthetic indexes determination showed that the photosynthetic rate of the 1Ns addition line had reached at 23.73 μmol/(m2·s), made the largest contribution, with a significant difference (P＜0.05) from wheat 7182 (15.73 μmol/(m2·s)) and suggesting a significantly positive effect. However the 3Ns (11.10 μmol/(m2·s)) and 6Ns (11.64 μmol/(m2·s)) addition line made a least contribution, suggesting a significantly negative effect. All results showed that the 6Ns and 1Ns chromosomes might have the genes which can promote young ear development and high photosynthesis efficiency, and can play an important role in germplasm improvement of existing varieties.
|
|
Received: 19 January 2015
Published: 01 June 2015
|
|
|
|
| 郜庆炉, 薛香, 吴玉娥, 等. 2003. 暖冬条件下播期对不同类型小麦幼穗分化的影响. 应用生态学报, 14(10): 1627-1631. （Gao Q L, Xue X, Wu Y E, et al. 2003. Effects of sowing times on the spike differentiation of different wheat varieties under the elimate of warm winter. Chinese Journal Of Applied Ecology, 14(10): 1627-1631.)匡猛,邢国风. 2005. 冬小麦春化研究进展. 西南科技大学学报(自然科学版), 20(3): 61-66. (Kuang M, Xing G F. 2005. Research on winter wheat vernalization. Journal Of Southwest University of Science and Tevhnology, 20(3): 61-66. )傅杰, 赵继新, 陈漱阳, 等. 2003. 小麦-华山新麦草抗全蚀病新种质的分子细胞遗传学研究. 西北植物学报, 23(11): 1904-1908. （Fu J, Zhao J X, Chen S Y, et al. 2003. Study on molecular cytogenetics of wheat-Psathyrostachys huashanica germplasm with resistance to wheat take-all fungus. Acta Bot. Boreal.-Occident. Sin, 23(11): 1904-1908.）Bdane C. 1991. A taxonomic revision of Psathyrostachys (Poaceae). Nord J Bot, 11(1): 3-26.郭本兆. 1987. 中国植物志（第九卷第三分册）. 北京:科学出版社. (Guo B Z. 1987. The flora of China (the archies of 9 (3)). Bei Jing: Science Press.)刘佩, 杨敏娜, 周新力, 吴会杰, 等. 2008. 普通小麦-华山新麦草易位系H9020-1-6-8-3抗条锈病基因的遗传分析和SSR标记. 植物病理学报, 38(1): 104-107. （Liu P, Yang M N, Zhou X L, Wu H J, et al. 2008. Genetic analysis and molecular mapping of stripe rust resistance of wheat translocation line H9020-1-6-8-3 derived from Psathyrostachys huashanica Keng. Acta Phytopathplxkgica, 38(1): 104-107.）Du W L, Wang J, Lu M, et al. 2013. Molecular cytogenetic identification of a wheat–Psathyrostachys huashanica Keng 5Ns disomic addition line with stripe rust resistance. Molecular Breeding, 31(4): 879-888.陈士强, 黄泽峰, 张勇, 等. 2012. 中国春背景下长穗偃麦草抗赤霉病相关基因的染色体定位. 麦类作物学报, 32(5): 839-845. （Chen S Q, Huang Z F, Zhang Y, et al. 2012. Chromosomal location of the genes associated with FHB resistance of lophopyrum elongatumin Chinese Spring background. Journal of Triticeae Crop, 32(5): 839-845.）陈漱阳, 张安静,傅杰. 1991. 普通小麦与华山新麦草的杂交. 遗传学报, 18(6): 508-512+579.(Chen S Y, Zhang A J, Fu J. 1991. The hybridiz ation between Tritcum aestivum and Psathyr ostachys huashanica. Acta Genetica Sinica, 18(6): 508-512+579.)陈漱阳, 侯文胜, 张安静, 等. 1996. 普通小麦－华山新麦草异附加系的选育及细胞遗传学研究. 遗传学报, 23(6): 447-452+488. (Chen S Y, Hou W S, Zhang A J, et al. 1996. Breeding and cytogenetic study of Triticum aestivum-Psathyrostachys huashanica alien addtion lines. Acta Genetica Sinica, 23(6): 447-452+488.)侯文胜, 张安静, 杨群慧, 等. 1997. 普通小麦-华山新麦草异代换系的选育及细胞遗传学研究. 西北植物学报, 17(3): 368-373.（Hou W S, Zhang A J, Yang Q H, et al. 1997. Breeding and cytogenetic study of Triticum aestivum-Psathyrostachys huashanica alien substitution lines. Acta Bot. Boreal, 17(3): 368-373 .赵继新, 陈新宏, 王小利, 等. 2004. 普通小麦-华山新麦草异代换系的分子细胞遗传学研究. 西北植物学报, 24(12): 2277-2281.（Zhao J X, Chen X H, Wang X L, et al. 2004. Molecular cytogentic study on the alien substitution lines of Triticum -Psathyrostachys. Acta Bot. Boreal. -Occident. Sin, 24(12): 2277-2281.）赵继新, 陈新宏, 王小利, 等. 2003. 普通小麦-华山新麦草异附加系的分子细胞遗传学研究. 西北农林科技大学学报(自然科学版), 32(11): 105-108+113. (Zhao J X, Chen X H, Wang X L, et al. 2003. Identification of the substitution and additional lines of Triticum -Psathyrostachys by C-banding. Jour. of North west Sci-Tech Univ. of Agri. and For. (Nat. Sci. Ed.), 31(6): 1-4.)武军, 赵继新, 陈新宏, 等. 2007. 普通小麦-华山新麦草衍生后代的细胞学特点及GISH分析. 麦类作物学报, 27(5): 772-775. （Wu J, Zhao J X, Chen X H, et al. 2007. Cytology characteristic and GISH analysis on the progenies derived from common wheat(T. aestivum L.)×Psathyrostachys huashanica. Journal of Triticeae Crops, 27(5): 772-775 .）Wang J, Lu M., Du W L, et al. 2013. A novel PCR-based marker for identifying Ns chromosomes in wheat-Psathyrostachys huashanica Keng derivative lines. Spanish Journal of Agricultural Research, 11(4): 1094.Du W L, Wang J, Pang Y H, et al. 2014. Development and application of PCR markers specific to the 1Ns chromosome of Psathyrostachys huashanica Keng with leaf rust resistance. Euphytica,白志英, 李存东, 孙红春, 等. 2008. 小麦代换系抗旱生理指标的主成分分析及综合评价. 中国农业科学, 41(12): 4264-4272. （Bai Z Y, Li C D, Sun H C, et al. 2008. Principal component analysis and comprehensive evaluation on physiological indices of drought resistance in wheat substitution. Scientia Agricultura Sinica, 41(12): 4264-4272 .）彭远英, 彭正松,宋会兴. 2005. 小麦中国春背景下长穗偃麦草光合作用相关基因的染色体定位. 中国农业科学, 38(11): 39-45. (Peng Y Y, Peng Z S, Song H X. 2005. Chromosomal location of the genes associated with photosynthesis of lophopyrum elongatum (Host) A.L?ve in Chinese Spring background. Scientia Agricultura Sinica, 38(11): 39-45.)Du W L, Wang J, Wang L M, et al. 2013. Development and Characterization of a Psathyrostachys huashanica Keng 7Ns Chromosome Addition Line with Leaf Rust Resistance. PLOS ONE, 8(8):Du W L, Wang J, Wang L M, et al. 2013. Isolation and Characterization of a Psathyrostachys huashanica Keng 6Ns Chromosome Addition in Common Wheat. PLOS ONE, 8(1)Du W L, Wang J, Lu M, et al. 2014. Characterization of a wheat-Psathyrostachys huashanica Keng 4Ns disomic addition line for enhanced tiller numbers and stripe rust resistance. Planta, 239(1): 97-105.Du W L, Wang J, Pang Y H, et al. 2014. Isolation and characterization of a wheat--Psathyrostachys huashanica 'Keng' 3Ns disomic addition line with resistance to stripe rust. Genome, 57(1): 37-44.Du W L, Wang J, Pang Y H, et al. 2014. Development and application of PCR markers specific to the 1Ns chromosome of Psathyrostachys huashanica Keng with leaf rust resistance. Euphytica, 200(2): 207-220.Du W L, Wang J, Wang L M, et al. 2014. Molecular characterization of a wheat-Psathyrostachys huashanica Keng 2Ns disomic addition line with resistance to stripe rust. Mol Genet Genomics, 289(5): 735-43.Cota-Sanchez J H, Remarchuk K, Ubayasene K. 2006. Ready-to-Use DNA Extracted with a CTAB Method Adapted for Herbarium Specimens and Mucilaginous Plant Tissue. Plant Molecular Biology Reporter, 24(161-167):曹广才, 吴东兵, 陈贺芹, 等. 2004. 温度和日照与春播小麦品质的关系. 中国农业科学, 37(5): 663-669. （Cao G C, Wu D B, Chen H Q, et al. 2004. Relationship between temperature, sunshine and quality of spring-sowing wheat. Scientia Agricultura Sinica, 37(5): 663-669.）Andra′s Farkas, Istva′n Molna′r, Tibor Kiss, et al. 2014. Effect of added barley chromosomes on the flowering time of new wheat/winter barley addition lines in various environments. Euphytica, 195: 45-55.T.T. Efremova, O.I. Maystrenko, V.S. Arbuzova, et al. 2006. Effect of alien 5R(5A) chromosome substitution on ear-emergence time and winter hardiness in wheat-rye substitution lines. Euphytica, 151: 145-153.张其德, 蒋高明, 朱新广, 等. 2001. 12个不同基因型冬小麦的光合能力. 植物生态学报, 25(5): 532-536. （Zhang Q D, Jiang G M, Zhu X G, et al. 2001. Photosynthetic capability of 12 genotypes of Triticum Aesticum. Acta Phytoecolog ica Sinica, 25(5): 532-536.） |
|
|
|
