温敏核雄性不育小麦中不育系和可育系花药基因的表达谱分析

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Abstract To reveal the molecular mechanism of the temperature/photoperiod sensitive male sterile lines of wheat (Triticum aestivum L.), the differently expressed genes in anthers at the tetrad and bicellular stage of the sterile and fertile lines of Bainong sterility (BNS) were analyzed using Affymetrix gene chip technology of wheat. The semi-quantitative RT-PCR was utilized to characterize some sterile candidate genes. The results showed that among the 61 127 probes transcripts, there were 418 valid transcripts presenting different expression between the sterile and fertile lines. Among these transcripts, 142 of them were down-regulated and 276 of them were up-regulated in the bicellular stage. Functional classification indicated that these genes participated in a series of significant life processes of metabolism process, cytological process, biological regulation and irritative reaction etc. Moreover, to verify chip data, 10 genes of leucine-rich repeats (Ta.17228.1), ATP-binding cassette transporter (Ta.21809.1), peptidoglycan binding protein (Ta.22570.1), Ta.37113.2(unknown), cytochrome P450 (Ta.19531.1), phosphoribulose kinase (Ta.18368.1), transcriptional regulator (Ta.9239.1), peptidase C1A subfamily (Ta.37113.1), thionin super family (Ta.21983.1) and V-type proton ATPase subunit D (Ta.18091.1), differentially expressed significantly, were identified by cDNA semi-quantitative RT-PCR. Result showed that the expression models of the 10 genes were concordant with the result of gene chip detection. Conservative structure domain analysis showed that Ta.18091.1, Ta.21809.1 and Ta.19531.1 most likely involved in the abortive anthers in the BNS sterile lines. The results provide important theoretical basis for sterility-related genes cloning and verification, and contribute to find the key gene which leads to pollen abortion in thermo-sensitive genic male sterile wheat (BNS).

Key words： Wheat Thermo-sensitive genic male sterility Bainong sterility (BNS) Gene chips Semi-quantitative RT-PCR

Received: 13 January 2015 Published: 01 June 2015

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	Articles by authors
	BO Yi-Xiong
	SONG Yu-Long
	NIU Yan-Bei
	YU Yong-Ang
	LIU Hong-Wei
	YU Xiao-Li
	MA Shou-Cai
	NIU Na
	ZHANG Gai-Sheng
	MA Ling-Jian
	ZHANG Xiao-Hong
	YU Jun-Wei

Cite this article:

BO Yi-Xiong,SONG Yu-Long,NIU Yan-Bei, et al. Analysis of Gene Expression Profiles of Sterile and Fertile Anthers of Thermo-sensitive Genic Male Sterile Wheat (Triticum aestivum L.)[J]. , 2015, 23(8): 1011-1019.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2015/V23/I8/1011

陈军营, 马平安, 赵一丹,等. 2009. 小麦成熟胚脱分化过程中生长素相关基因的表达分析[J]. 作物学报, 35(10):1798?1805. (Chen J Y, Ma P A, Zhao Y D, et al. Expression of Auxin-Related Genes during Dedifferentiation of Mature Embryo in Wheat[J]. Acta Agronomica Sinica, 35(10): 1798?1805.)
董剑, 刘迎团, 刘宏伟,等. 2013. 基因芯片分析杀雄剂SQ-1诱导小麦生理型雄性不育的基因差异表达[J]. 中国生物化学与分子生物学报, (10): 948-955. (Dong J, Liu Y T, Liu H W, et al. 2013. Transcriptomic Profiling of Physiological Male Sterility Induced by Chemical Hybridizing Agent SQ-1 in Wheat [J]. Chinese Journal of Biochemistry and Molecular Biology, (10): 948-955. )
杜士云,阳菁,王守海,等. 2010. 应用基因芯片分析短日低温条件下新型粳稻光温敏核质互作不育系育性相关基因.中国水稻科学, 24 ( 6) : 559-566. ( Du S Y, Yang J, Wang S H, et al. 2010. Analysis on sterility-related genes in a new type of japonica photo ( thermo) -sensitive male sterile line under short day length and low temperature conditions by microarray[J]. Chinese Journal of Rice Science, 24 ( 6) : 559-566.)
韩艳芬,张龙雨,胡俊敏,等. 2010. 黏类小麦细胞质雄性不育线粒体atp6基因转录本编辑位点[J].作物学报,36(12):2179-2184.(Han Y F, Zhang L Y, Hu J M, et al. Editing Sites in Transcript of Mitochondrial atp6 Gene of Male Sterile Line with Aegilops kotschyi Cytoplasm in Wheat[J]. Acta Agronomica Sinica, 36(12): 2179-2184.)
李东霄,邓小莉,茹振钢,等. 2012. 温敏核不育小麦可育花药和败育花药发育观察[J]. 中国细胞生物学学报,34(09):37-42.(Li D X, Deng X L, Ru Z G, et al. 2012. Observation in Fertile and Sterile Anthers of A Thermo-sensitive Genic Male-sterile Wheat [J]. Chinese Journal of Cell Biology, 34(09):37-42.).
李东霄, 李淦, 冯素伟,等. 2013. 温敏核不育小麦可育和不育花药的细胞化学观察[J]. 作物学报39(05):878-884（Li D X, Li G, Feng S W, et al. 2013. Cytochemical Observation of Fertile and Sterile Anthers of Thermo-Sensitive Genic Male-Sterile Wheat[J]. Acta Agronomica Sinica, 39(05):878-884,.
李罗江, 茹振刚, 高庆荣,等. BNS小麦的雄性不育性及其温光特性. 2009. 中国农业科学, 42(09): 3019-3027.（Li L J, Ru Z G, Gao Q R, et al．2009. Male Sterility and Thermo-Photosensitivity Characteristics of BNS in Wheat [J]. Scientia Agricultura Sinica, 42(09): 3019-3027.）
李友勇, 茹振钢, 苏晴,等. 2011. 小麦BNS雄性不育系及其转换系花药差异蛋白鉴定与分析[J]. 作物学报,37(09):1540-1550.（Li Y Y, Ru Z G, Su Q, et al. 2011. Identification and Analysis of Differentially Expressed Proteins of BNS Male Sterile Line and Its Conversion Line of Wheat[J]. Acta Agronomica Sinica, 37(09):1540-1550.）
宁江权, 茹振刚, 郑炜君, 等. 2011. BNS小麦雄性不育性表现及其恢复性的研究[J]. 麦类作物学报,31(4): 642-647.（Ning J Q, Ru Z G, Zheng W J, et al. 2011.Male Sterility and Restoration of Thermo photo sensitive Male Sterile Line BNS of Common Wheat [J]. Journal of Triticeae Crops, 31(4): 642-647.）
邱星辉,冷欣夫. 2002. 细胞色素P450在植物与昆虫相互关系中的作用[J].昆虫知识, 39(01):5-8. (Qiu X H, Leng X F. The role of cytochrome P450 in plant-insect interaction Chinese Bulletin of Entomology[J]. Entomological Knowledge), 39(01):5-8.
苏晴,秦志英,程威,等. 2011. 小麦BNS雄性不育系小孢子发育形态结构的细胞学观察[J]. 河南科技学院学报(自然科学版), 39(06):5-9.( Su Q, Qin Z Y, Cheng W, et al. 2011. Morphological and structural observations on anther and microspore developments of male sterile line BNS in wheat[J]. Journal of Henan Institute of Science and Technology, 39(06):5-9.)
王震, 范晓静, 张淼,等. 2014. ATP合成相关基因在小麦BNS不育系育性转换中的差异表达[J]. 作物学报, 08:1501-1505.（Wang Z, Fan X J, Zhang M, et al. 2014. Differential Expression of ATP Synthesis Related Gene in Fertility Conversion of Wheat BNS Male Sterile Line [J]. Acta Agronomica Sinica, 08:1501-1505.）
姚雅琴,张改生,刘宏伟,等. 2002. K型和T型小麦雄性不育花粉粒形态与细胞化学定位[J]. 中国农业科学, 35 (02): 123-126. (Yao Y Q, Zhang G S, Liu H W, et al. Cytomorphology and Cytochemical Localization of K-type and T-type Cytoplasmic Male Sterile Pollens in Wheat[J]. Scientia Agricultura Sinica),35 (02): 123-126.)
张保雷,张卫东,高庆荣,等. 温光敏雄性不育小麦BNS育性的遗传效应分析. 中国农业科学, 46(08): 1533-1542.（Zhang B L, Zhang W D, GAO Q R, et al. 2013. Genetic Analysis on Male Sterility of Thermo Photo Sensitive Male Sterile Line BNS in Wheat [J]. Scientia Agricultura Sinica, 46(08): 1533-1542.）
张龙雨,李红霞,张改生,等. 2009. 黏类小麦细胞质雄性不育相关基因cMDH的克隆与表达分析 [J]. 作物学报,35(09): 1620?1627. (Zhang L Y, Li H X, Zhang G S, et al. Cloning and Expression Analysis of cMDH Gene Related to Cytoplasmic Male Sterile Wheat with Aegilops kotschyi Cytoplasm[J]. Acta Agronomica Sinica, 35(09): 1620?1627.)
张玉红,安志刚. 2013.植物V-H~+-ATP酶适应逆境的分子调控机理[J]. 草业科学, 30(02):245-252. (Zhang Y H, An Z G. 2013.Molecular regulation mechanism of V-H+ -ATPase adapt to environmental stress in plant[J]. Pratacultural Science, 30(02):245-252. )
张自阳,胡铁柱,冯素伟,等. 2010. 温敏核雄性不育小麦BNS的育性转换规律初探[J]. 河南农业科学, 07:5-9.（Zhang Z Y, Hu T Z, Feng S W, et al. 2010. A Preliminary Study on Fertility Alteration of Thermo-sensitive Genic Male S terile Wheat Line BNS [J] . Journal of HeNan Agricutural Sciences,07:5-9.）
赵宝存, 赵芊, 葛荣朝,等. 2007.利用基因芯片研究小麦耐盐突变体盐胁迫条件下基因的表达图谱[J].中国农业科学, 40(10): 2355-2360. (Zhao B C, Zhao Q, Ge R C, et al. Study on the Expression Profile of Salt-Tolerance Mutant Under Salt-Stress in Wheat Using Gene Microarray[J]. Scientia Agricultura Sinica, 40(10): 2355-2360. )
Bai X N, Fu R H, Ming H, et al. 2013. The ATP-binding Cassette Transporter OsABCG15 is Required for Anther Development and Pollen Fertility in Rice[J]. Journal of Integrative Plant Biology. 55(8):710-720.
Cao S H, Liu D C, Liu L K, et al. 2003. Differential Expression of Genes Related to Photoperiod temperature Sensitive Genic Male Sterility in Wheat, Revealed by mRNA Differential Display Using G-box Family Primer[J]. Acta Genetic Sinica, 30(1): 56-61.
Chapple C. 1998. Molecular-genetic analysis of plant cytochrome p450-dependent monooxygenases [J]. Annual Reviews of Plant Biology, 49: 311-343.
Klaus W. Beyenbach, Helmut W. 2006. Review The V-type H+ ATPase: molecular structure and function, physiological roles and regulation[J]. The Journal of Experimental Biology, 209, 577-589.
Lu Z, Jian X S, Guo C Z, et al. 2013. Post-meiotic Deficient Anther1 (PDA1) Encodes an ABC Transporter Required for the Development of Anther Cuticle and Pollen Exine in Rice[J]. Journal of Plant Biology. 56(1) :59-68.
Luo D, Xu H, Liu Z L, et al. 2013.A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice[J]. Nature Genetics, 45(5): 573-577.
Nanjo T, Fujita M, Seki M, et al. 2003. Toxicity of free proline revealed in a Arabidopsis T-DNA Tagged mutant deficient in proline dehydrogenase[J]. Plant Cell Physiology, 44(5): 541–548.
Ohkawa H , Imaishi H, Shiota N, et al . 1998. Molecular mechanisms of herbicide resistance with special emphasis on cytochrome P450 monooxygenases[J] . Plant Biotechnology journal, 15( 4) : 173-176.
Ruepp A , Zollner A , Maier D, et al. 2004. The FunCat, a functional annotation scheme for systematic classification of proteins from whole genomes[J]. Nucleic Acids Research, 32(18):5539- 5545.
Xiang W S, Wang X J, Ren T R, et al. 2006. Expression of a wheat cytochrome P450 monooxygenase cDNA in yeas t catalyzes the metabolism of sulfonylurea herbicides[J]. Pesticide Biochemistry Physiology , 85(01): 1-6.
Yasunari O, Yosuke K, Kazunobu F, et al. 1999. Photoperiod-sensitive cytoplasmic male sterility in wheat: nuclear-mitochondrial incompatibility results in differential processing of the mitochondrial orf25 gene [J]. Current Genetics, 36(6): 354-362.
Zhang H, Liang W Q, Yang X J, et al. 2010. Carbon Starved Anther Encodes a MYB Domain Protein That Regulates Sugar Partitioning Required for Rice Pollen Development[J]. The Plant Cell, 22(3): 672-689.
Zhang X.W, Lei J J, Yan Z, et al. 2013. In Planta Stage-Specific Fungal Gene Profiling Elucidates the Molecular Strategies of Fusarium graminearum Growing inside Wheat Coleoptiles. The Plant Cell, 24(12): 5159-5176.