生长激素受体(GHR)在牦牛不同组织中的表达分析

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Abstract To explore the expression of growth hormone receptor (GHR) in different tissues of yak(Bos grunneins), samples of these tissues (heart, liver, spleen, lung, kidney and testis) were collected from healthy yaks under normal physiological conditions. Quantitative Real-time PCR (qRT-PCR) was applied to analyze the expression of GHR gene in different tissues. Immunohistochemistry (IHC) was used to detect the distribution and localization of GHR protein, integrated optical density (IOD) of GHR immunoreactions were measured, and followed by an analysis for significant difference. The results of qRT-PCR showed that GHR gene had a differential expression in different tissues, comparative expression quantity of GHR gene in liver was 3.73 fold than that in heart, 4.98 fold in kidney, 5.03 fold in testis, 8.58 fold in lung and 10.21 fold in spleen, respectively. Unequal immunoreaction intensity was observed in different tissues by immunohistochemistry. Comparative analysis of IOD showed that GHR had the strongest immunoreactions in liver, and followed by the heart, kidney, testis and spleen, the weakest immunoreactions of GHR was found in lung. This experiment demonstrated that GHR had a differential expression in different tissues by studying at genetic and protein level. This work provides a theoretical information for improvement and breeding of yak.

Key words： Growth hormone receptor (GHR) Yak Different tissues qRT-PCR Immunohistochemistry

Received: 29 December 2014 Published: 09 July 2015

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	Articles by authors
	XIONG Li
	CHEN Hong
	PAN Yang-Yang
	HU Wei
	YUN Ze-Xing
	XUN Zheng-Dong
	FAN Jiang-Feng
	CUI Yan
	YU Si-Jiu

Cite this article:

XIONG Li,CHEN Hong,PAN Yang-Yang, et al. Expression Analysis for Growth Hormone Receptor (GHR) in Different Tissues of Yak (Bos grunneins)[J]. , 2015, 23(9): 1234-1239.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2015/V23/I9/1234

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