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| Analysis of SNP Markers for Blue-shelled Gene in Chicken by PCR-SSCP |
| ZHAO Rui;LIU Zhen-zhen;XU Gui-yun;YANG Ning |
| College of Animal Science and Technology, China Agricultural University, Beijing 100094, China |
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Abstract Oocyan(O) gene determines blue shell pigmentation of domesticated chicken. O locus is closely linked to ALEV1 on chromosome1 with a genetic distance of 2.3 cM. Blue eggshells contain protoporphyrin, biliverdin and zink biliverdin chelatate, which belong to the porphyrin metabolic pathway. A bioinformatic approach was first used to blast the genes for enzymes of porphyrin pathway and no gene was detected to specify O locus. Due to limited number of SSR markers around O locus, PCR-SSCP was then employed to search for candidate SNP markers. The sequence bearing a marker was found to be consisted of two transversions at Chr1:61754089T->A and Chr1:61754175A->T, respectively. Population screening showed that 81% of homozygous blue-shelled layers(OO) were classified as AA genotype at the SNP loci, while 89% of heterozygous blue-shelled layers(Oo) were AB genotype and 93% of tinted layers(oo) were BB genotype. The results indicated that there was a close association between O locus and SNP locus, which suggests that the marker locus can be used as a molecular marker in breeding for blue-shelled chickens.
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Received: 13 September 2005
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Corresponding Authors:
YANG Ning
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