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| Analysis of a 412 insertion mutation at scarlet locus of outcross progeny of Bar fly |
| YANG Jun;LIAN Lin-sheng;ZHAO Chun-jiang;BAI Li-hua;WU Chang-xin |
| College of Animal Science and Technology, China Agricultural University，Beijing 100094，China |
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Abstract Six pairs of oligonucleotide primers were designed to amplify and sequence scarlet gene of mutant stbr flies which were obtained by outcross between Bar and wild type flies. A 7.5-kb insertion identified as 412 element was detected at 4th exon of scarlet gene. Heterozygous 412 insertion and homozygous 412 insertion were also detected at scarlet gene of Bar and st1 flies imported from Japan respectively. The 412 insertion site of stbr was at 1612thbp-1613thbp of scalet gene with a 450-bp loss of 5´ LTR but the insertion of st1 was at 1722ndbp-1723rdbp
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Received: 11 April 2006
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Corresponding Authors:
WU Chang-xin
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