Proteomic Analysis of Hemolymph in Bombus lantschouensis Egg-laying Workers
DONG Jie1, HUANG Min-Jie1, WU Jie2, HAN Lei2, WANG De-Qian1*
1 Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2 Institute of Apicultural Research/Key Laboratory for Insect-Pollinator Biology of the Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100093, China
Abstract The phenomenon of worker bee ovary development and egg laying in bumblebees indicates the decline of bee colony development. The worker bees' division of labor has shifted from active collection before egg laying to full-time egg laying, which will have a negative impact on the collection ability and pollination effect of bee colonies. In order to explore the regulation mechanism of egg-laying in workers, label- free quantitative proteomics was used to identify and compare differentially expressed proteins (DEPs) in the hemolymph of egg-laying and non-egg-laying workers of Bombus lantschouensis, then the DEPs were analyzed for GO classification and KEGG pathway enrichment, and the transcription levels of 6 randomly selected DEPs were verified by qRT-PCR. The results showed that a total of 370 DEPs were identified, including 212 up-regulated and 158 down-regulated proteins in hemolymph of egg-laying workers. GO function annotation showed that the largest number of DEGs were metabolism process (61 DEGs), followed by catalytic activity (60 DEGs) and binding (50 DEGs). DEPs enriched in detoxification, immune and antioxidant activity were up- regulated. The KEGG pathway enrichment analysis result showed that DEGs were significantly enriched in energy-related metabolism pathways including glycolysis/gluconeogenesis, carbon metabolism, fatty acid oxidation, glyoxylic acid and dicarboxylic acid metabolism, and protein synthesis-related pathways including extracellular matrix receptor interaction, ribosome, proteasome. In addition, DEPs were also significantly enriched in Notch and Hippo signaling pathway related to insect growth and development. The change trend of 6 randomly selected DEPs at transcription level was consistent with the results of proteomics. This study provides basic data for in-depth exploration of the molecular mechanism of oviposition regulation in B. lantschouensis workers, and some theoretical guidance for achieving inhibiting oviposition of workers by artificial regulation.
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