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农业生物技术学报  2023, Vol. 31 Issue (6): 1182-1192    DOI: 10.3969/j.issn.1674-7968.2023.06.007
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Mining Key Genes Affecting Muscle Water Retention of Gannan Dzo (Bos taurus) Based on RNA-seq Technology
WANG Chang-Feng1, HAN Ling1, LI Ai-Xia1, YU Qun-Li1,*, ZHANG Li1,*, SHI Hong-Mei2, KONG Xiang-Ying3, ZHU Xiao-Peng4, ZHANG Xin-Jun5
1 College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China;
2 Gansu Gannan Animal Husbandry and Veterinary Workstation, Gannan 747000, China;
3 Qinghai Haibei Animal Husbandry and Veterinary Science Research Institute, Haibei 812200, China;
4 Gansu Wanhe Grass and Livestock Industry Technology Development Co., Ltd., Lanzhou 730070, China;
5 Ningxia Xiahua Meat Food Co., Ltd., Zhongwei 75500, China
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Abstract  Muscle water retention is one of the important characteristics of edible meat quality, and greatly affects the sensory characteristics and economic value of meat. In order to screen the differentially expressed genes associated with muscle water retention of Gannan dzo (Bos taurus), 3 yaks (Bos grunniens) and 3 dzoes, each with healthy growth and development, equivalent slaughter time and even weight, were slaughtered in line with standardization. The longissimus dorsi muscle was collected as the experimental material for the determination of cooking loss, pressure loss, dripping loss and RNA-seq transcriptome sequencing. The differential expression multiplier value |log2 (Fold change)|≥1 and the significance level P<0.05 were used as the conditions for selecting the differential expression genes. The cooking loss and dripping loss of yak were significantly higher than that of dzo (P<0.05), and the pressure loss was significantly lower than that of dzo (P<0.05). A total of 748 differentially expressed genes were obtained from the sequencing results, including 526 down-regulated and 222 up-regulated genes. In order to verify the reliability of the sequencing data, 6 differentially expressed genes were randomly selected for qRT-PCR verification. The gene expression trend was consistent with the transcriptome sequencing results, indicating that the sequencing results were reliable. GO function annotation and KEGG pathway enrichment analysis found that the items related to muscle water retention included fibrinolysis, protein decomposition, glucose metabolism and regulation of actin cytoskeleton, and the pathways involved in water retention include glycolysis/gluconeogenesis, peroxisome proliferator activated receptor (PPAR) and AMP-activated protein kinase (AMPK). A total of 55 nonredundant differentially expressed genes were obtained, of which troponin T type 2 (TNNT2), bone morphogenetic protein 1 (BMP1), fatty acid-binding protein 1 (FABP1) and low-density lipoprotein receptor- related protein-1 (LRP1) were related to the water retention of dzo, which could be used as candidate genes for genetic and breeding improvement of meat quality traits. This study provides basic data for further research on the molecular mechanism of water retention of bovine muscle.
Key wordsDzo      RNA-seq      Differentially expressed gene      Water retention      Muscle     
Received: 09 August 2022     
Corresponding Authors: *yuqunlihl@163.com; zhanglwubd@163.com   
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Articles by authors
WANG Chang-Feng
HAN Ling
LI Ai-Xia
YU Qun-Li
ZHANG Li
SHI Hong-Mei
KONG Xiang-Ying
ZHU Xiao-Peng
ZHANG Xin-Jun
Cite this article:   
WANG Chang-Feng,HAN Ling,LI Ai-Xia, et al. Mining Key Genes Affecting Muscle Water Retention of Gannan Dzo (Bos taurus) Based on RNA-seq Technology[J]. 农业生物技术学报, 2023, 31(6): 1182-1192.
URL:  
http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2023.06.007     OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2023/V31/I6/1182
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